Ask about this productRelated genes to: Gnpda1 Blocking Peptide
- Gene:
- GNPDA1 NIH gene
- Name:
- glucosamine-6-phosphate deaminase 1
- Previous symbol:
- GNPI
- Synonyms:
- GNPDA, HLN, GPI, KIAA0060
- Chromosome:
- 5q31.3
- Locus Type:
- gene with protein product
- Date approved:
- 1999-09-09
- Date modifiied:
- 2016-10-05
Related products to: Gnpda1 Blocking Peptide
Related articles to: Gnpda1 Blocking Peptide
- This study investigated the biological functions and molecular mechanisms of RNA-binding motif protein 11 (RBM11) in bladder cancer (BCa) progression. Integrated bioinformatics analysis of the TCGA database and validation in clinical tissues revealed that RBM11 is significantly upregulated in BCa and positively correlated with advanced tumor stage, poor prognosis, and epithelial-mesenchymal transition (EMT). RBM11 knockdown effectively suppressed migration, invasion, proliferation, and chemoresistance of BCa cells, whereas RBM11 overexpression produced opposite effects. Mechanistically, RBM11 promotes GNPDA1 expression by regulating alternative splicing of GNPDA. Furthermore, GNPDA1 directly interacts with PKM2 and inhibits its ubiquitin-proteasome-mediated degradation, thereby stabilizing PKM2 protein levels, enhancing glycolysis, and promoting malignant progression of BCa. Collectively, these findings indicate that RBM11 drives malignant progression of BCa through the GNPDA1-PKM2 axis, enhancing glucose metabolism reprogramming and EMT process, suggesting that RBM11 may be a potential therapeutic target for BCa. - Source: PubMed
Publication date: 2026/03/17
Tong HangLi TinghaoZhu JunlongDou QianYu QiongSun YanHe Weiyang - OBJECTIVES: The aim of this study is to investigate whether tongue squamous cell carcinoma (TSCC)-derived exosomal microRNAs (miRNAs) promote the conversion to tumor-associated fibroblasts (CAFs) and to elucidate the underlying molecular mechanisms. METHODS: First, we isolated and characterised exosomes from the CAL27 tongue squamous cell carcinoma cell line. These exosomes were then co-cultured with human oral mucosal fibroblasts (hOMFs). We evaluated the expression of inflammatory cancer-associated fibroblasts (iCAFs)-related markers using Western blotting (WB), quantitative real-time PCR (qRT-PCR) and ELISA. We then conducted miRNA sequencing of the exosomes to identify the top five highly expressed miRNAs. WB was then employed to assess which of the corresponding miRNA mimics most effectively promotes the transformation of hOMFs into iCAFs. Finally, we predicted the potential target genes of the selected miRNA through bioinformatics analysis and validated them using a dual-luciferase reporter assay. RESULTS: Under transmission electron microscopy, the isolated exosomes exhibited a characteristic cup-shaped morphology with a double-membrane structure. Nanoparticle tracking analysis (NTA) revealed a size distribution ranging from 30 to 150 nm. Western blotting confirmed positive expression of the exosomal markers CD9, CD81, HSP90 and TSG101, while microRNA (miRNA) sequencing identified the most highly expressed miRNAs as miR-21-5p, miR-122-5p, miR-148a-3p, miR-143-3p and let-7i-5p. Of these, miR-122-5p induced the most significant changes in the expression levels of iCAF-related markers. Bioinformatic prediction combined with dual-luciferase reporter assays confirmed that GNPDA1 is a direct target of miR-122-5p. CONCLUSION: CAL27-exo may facilitate the conversion of hOMFs into iCAFs by targeting glucosamine-6-phosphate deaminase 1 (GNPDA1) via exosomal miR-122-5p. - Source: PubMed
Publication date: 2026/03/10
Xia BinWu JiaminZhang HongrongYang HefengXu Biao - To evaluate the correlation between glucosamine-6-phosphate isomerase 1 (GNPDA1) expression and prognosis, immune infiltration, and immune evasion in head and neck squamous cell carcinoma (HNSCC). We analyzed the expression of GNPDA1 in HNSCC tissues and obtained RNA sequence data from the Cancer Genome Atlas (TCGA) database. Kaplan-Meier survival analysis evaluated the relationship between GNPDA1 expression and advanced tumor stage, TNM stage, grading, and gender. Co-expressed genes with GNPDA1 were identified using TCGA data and annotated through gene ontology and Kyoto encyclopedia of genes and genomes analyses. A protein-protein interaction network was constructed using the STRING database. Single-sample gene set enrichment analysis was conducted based on TCGA and TIMER 2.0 databases to assess the correlation between GNPDA1 and immune infiltration. In addition, the location of GNPDA1 in tumor cell and immune cell structures was identified by the tumor immune stromal cells helper database, and potential protein-interacting molecules of GNPDA1 were elucidated in the STRING database. Potential GNPDA1 gene functions were assessed using gene set enrichment analysis. Our results indicate that the expression of GNPDA1 is elevated in HNSCC tissues (P < .05). GNPDA1 expression was positively correlated with tumor malignancy (P < .05) and negatively correlated with patient prognosis (P < .05). There was a significant correlation between high expression of GNPDA1 and advanced tumor stage, N-stage, or G-grade, and it was associated with gender. High GNPDA1 expression was associated with increased infiltration of resting CD4+T cells, macrophages M1 and M2, resting natural killer cells, monocytes, eosinophils, and naïve B cells (P < .05). In contrast, low GNPDA1 expression was associated with increased infiltration of activated natural killer cells, neutrophils, activated mast cells, macrophages M0, plasma cells, activated dendritic cells, CD8+T cells, memory B cells, regulatory T cells (Tregs), and naïve CD4+T cells (P < .05). In addition, GNPDA1 was observed to be closely associated with various immune evasion-related genes in HNSCC. The results of this study suggest that GNPDA1 can serve as a potential prognostic marker and therapeutic target for HNSCC and may be a key gene mediating immune evasion in HNSCC. - Source: PubMed
Liu PengYang DunhuiMa Ruixia - Biopsychosocial factors, including family history, influence the development of breast cancer. Malignancies in women with a family history of breast cancer may be detectable based on DNA methylation and microRNA. : The present study extended an integrative analysis of DNA methylation and microRNA to identify genes associated with biopsychosocial factors. : We identified 3060 healthy women from the Taiwan Biobank and included 32 blood plasma samples for analysis of biopsychosocial factors and epigenetic changes. GEO databases and bioinformatics approaches were used for the identification and validation of potential genes. : Our integrative analysis revealed GNPDA1 and SLC25A16 as potential genes. Age, a family history of cancer, and alcohol consumption were associated with GNPDA1 and SLC25A16 based on the current data set and the GEO data set. GNPDA1 and SLC25A16 exhibited significant expression in breast cancer tissues based on UALCAN analysis, where they were overexpressed and underexpressed, respectively. Through a MethSurv analysis, GNPDA1 hypomethylation and SLC25A16 hypermethylation were associated with poor prognoses in terms of overall survival in breast cancer. Moreover, through a MetaCore functional enrichment analysis, GNPDA1 and SLC25A16 were associated with the BRCA1, BRCA2, and pro-oncogenic actions of the androgen receptor in breast cancer. Further, GNPDA1 and SLC25A16 were enriched in known targets of approved cancer drugs as potential genes associated with breast cancer. : These two genes might serve as biomarkers for the early detection of breast cancer, especially for women with a family history of breast cancer. - Source: PubMed
Publication date: 2025/03/30
Khairi SabiahWang Chih-YangAnuraga GanggaPrayugo Fidelia BereniceAnsar MuhamadLesmana Mohammad Hendra SetiaIrham Lalu MuhammadShen Chen-YangChung Min-Huey - Nearly 5% of the glucose-6-phosphate (Glc6P) in cells is diverted into the hexosamine biosynthetic pathway (HBP) to synthesize glucosamine-6-phosphate (GlcN6P) and uridine diphosphate -acetyl-glucosamine-6-phosphate (UDP-GlcN6P). Fructose-6-phosphate (Fru6P) is a common intermediary between glycolysis and the HBP. Changes in HBP regulation cause abnormal protein N-glycosylation and -linked-N-acetylglucosamine modification (O-GlcNAcylation), affecting protein function and modifying cellular responses to signals. The HBP enzymes glucosamine-6-phosphate deaminases 1 and 2 (GNPDA1 and 2) turn GlcN6P back into Fru6P and ammonium, and have been implicated in cancer and metabolic diseases. Despite the plentiful literature on this topic, the mechanisms involved are just beginning to be studied. In this review, we summarize, for the first time, the current knowledge regarding the possible roles of the isoenzymes of both GNPDAs in the pathogenesis and development of metabolic diseases and cancer from a molecular point of view, highlighting their importance not only in supplying carbon from glycolysis, but also in ammonia metabolism. - Source: PubMed
Publication date: 2024/11/09
Lara-Lemus RobertoCastillejos-López ManuelAquino-Gálvez Arnoldo