Ask about this productRelated genes to: HSD3B7 Blocking Peptide
- Gene:
- HSD3B7 NIH gene
- Name:
- hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 7
- Previous symbol:
- -
- Synonyms:
- C(27)-3BETA-HSD, SDR11E3
- Chromosome:
- 16p11.2
- Locus Type:
- gene with protein product
- Date approved:
- 2003-01-13
- Date modifiied:
- 2016-07-18
Related products to: HSD3B7 Blocking Peptide
Related articles to: HSD3B7 Blocking Peptide
- Steroid hormones regulate ovarian development, but hsd3b7 function in fish remains unclear. In this study, we cloned full-length hsd3b7 cDNA from Qihe gibel carp (Carassius gibelio var. Qihe) and generated a polyclonal antibody. Real-time PCR, Western blotting, and immunohistochemistry revealed widespread hsd3b7 expression in brain, heart, spleen, muscle and ovary. During ovarian development and maturation, hsd3b7 mRNA expression peaked at 150 days after hatching (dah) and remained at a relatively high level until 720 dah. Immunohistochemical analysis further revealed that Hsd3b7 protein was localized in both gonadal germ cells and somatic cells, suggesting its involvement in ovarian development and maturation. To further investigate its function, hsd3b7 was knocked down for 30 days by ovarian injection of double-stranded RNA (dsRNA) during the previtellogenic stage. Silencing of hsd3b7 markedly inhibited ovarian development, with most oocytes remaining at phase II. In addition, the expression levels of the germ cell marker genes vasa and piwil, as well as the cell proliferation-related genes pcna and ki-67, were significantly reduced. Protein interaction analysis suggested that Hsd3b7 is associated with several steroidogenic enzymes, including Cyp19a1a, Cyp11c1, Hsd17b3, and Hsd17b7. Moreover, hsd3b7 knockdown altered the expression of genes involved in steroidogenesis and lipid accumulation, and significantly affected serum E2 and total cholesterol levels. Collectively, these findings indicate hsd3b7 regulates ovarian development and maturation via steroid hormone synthesis and lipid metabolism, providing insights for addressing precocious maturation in Qihe gibel carp. - Source: PubMed
Publication date: 2026/08/13
Li YongjingJin YujiaoLi YuqiXu YufengLi XuejunWu Limin - 3β-Hydroxysteroid dehydrogenases (3βHSDs) are key enzymes in steroid metabolism, catalyzing C3 oxidation-reduction and Δ5→Δ4 isomerization reactions that govern metabolic flux across multiple steroidogenic pathways. However, the functional diversity of 3βHSDs involved in bufadienolide metabolism in amphibians remains poorly explored. Here, we systematically characterized the 3βHSD gene family in the Asian toad (Bufo bufo gargarizans) using integrated transcriptomic, biochemical, and metabolomic analyses. Seven Bg-3βHSD genes were identified from multi-tissue transcriptomes generated under control and Pb2+ exposure conditions, and six were heterologously expressed for functional evaluation. In vitro assays revealed pronounced functional divergence among Bg-3βHSD isoforms. Bg-3βHSD1 primarily catalyzed bidirectional C3 redox reactions of C21 steroids and bile acid-related substrates, consistent with canonical steroidogenic roles. In contrast, Bg-3βHSD2 enzyme exhibited broad substrate specificity and high catalytic efficiency toward hormones, bile acids, and bufadienolides. In addition to canonical C3 redox reactions and Δ5→Δ4 isomerization, Bg-3βHSD2 also displayed additional oxidation activity at the C17 position for several steroid substrates. A third homolog, Bg-HSD3B7 (GenBank accession no. XM 044303756.1), selectively converted 7α-hydroxylated sterols, suggesting a potential role in classical bile acid metabolism. Integration of tissue-specific expression profiles with bufadienolide distribution patterns suggests that Bg-3βHSD2 may contribute to connecting classical steroid metabolism with bufadienolide biosynthesis in adrenal tissue. Together, the present study identifies Bg-3βHSD2 as an efficient and versatile steroid-transforming enzyme, expands our knowledge of functional diversity within the amphibian 3βHSD family, and provides insights into the enzymatic basis of steroid and bufadienolide metabolism in B. bufo gargarizans. - Source: PubMed
Meng QianHu YaotingChen WenjieXiao ChuanguangYang LiWang RufengZhang BeibeiZhao ShujuanWang Zhengtao - : Bile acid synthesis disorders (BASDs) represent a distinct category of progressive familiar cholestatic liver disease. A novel targeted mass spectrometry assay was developed for the accurate measurement of the major urinary atypical bile acids and bile alcohols that are biomarkers for HSD3B7, AKR1D1, CYP7B1 and CYP27A1 deficiencies, the four most common BASDs. Stable-isotope dilution UPLC tandem mass spectrometry was used for the simultaneous quantification of 12 key atypical bile acid biomarkers in urine from patients with BASD. Typical concentration ranges for these metabolites were established from urine samples from patients with biochemically and/or genetically confirmed BASD and compared with non-cholestatic and cholestatic controls. The separation of major 3β-hydroxy-Δ-bile acid sulfates, taurine- and glycine-conjugated 3-oxo-Δ-bile acids, and bile alcohol glucuronides was achieved in a 20 min chromatographic run with intra- and inter-batch imprecisions of <15% for all metabolites. The mean ± SEM urinary concentration of total 3β-sulfated-Δ-cholenoic acids in patients with HSD3B7 deficiency was 704 ± 204 µmol/L (n = 22), approximately 2000-fold higher than in cholestastic patients (n = 168) or non-cholestatic controls (n = 127). Similarly, the concentration of 5β-cholestane-3α,7α,12α,24,25-pentol-glucuronide, the major bile alcohol, in patients with CYP27A1 deficiency was 95 ± 17 µmol/L (n = 12). For CYP7B1 deficiency, two confirmed cases showed elevated levels (average, 7.5 µmol/L) of the glycine conjugate of 3β-sulfooxy-Δ-bile acid. In AKR1D1 deficiency, total 3-oxo-Δ-bile acids in urine were elevated (81 ± 16 µmol/L, n = 48), but concentrations showed overlap with cholestatic and non-cholestatic controls. : A novel quantitative tandem mass spectrometry assay is described for the measurement of the major atypical metabolites and biomarkers in urine applicable to the accurate monitoring of treatment responses, and for the first time typical concentration ranges are established for each of these BASDs. - Source: PubMed
Publication date: 2026/06/23
Setchell Kenneth D RZhao XuehengReed StaceyZhang Wujuan - Food-responsive enteropathy (FRE) is a common form of chronic inflammatory enteropathy in dogs. Its underlying molecular mechanisms remain incompletely characterized. Increasing evidence from human studies and emerging canine data suggests that bile acids (BAs) influence intestinal homeostasis and inflammation. Duodenal mucosal biopsies from dogs with FRE ( = 8) and healthy controls ( = 4) were analyzed by bulk RNA sequencing. Differential expression analysis (DESeq2), KEGG and Reactome pathway enrichment, and GSEA were performed with a specific focus on BA transport, sensing, and metabolic pathways. FRE samples showed a distinct BA-associated transcriptional signature, including a non-significant decreasing trend of the BA receptor NR1H4 (FXR) expression (padj = 0.057), and significant upregulation of the nuclear receptor RXRA, together with increased expression of downstream mediators NR0B2 (SHP) and FGF19. BA transport components such as SLC51A (OSTα) and ABCC3 (MRP3) were differentially regulated, and the BA-synthetic enzyme HSD3B7 was increased. Bile secretion was among the top enriched KEGG pathways (ranked 9th; NES = 1.935; padj = 0.005). This study provides, to our knowledge, the first focused mucosal transcriptomic evidence of coordinated BA-axis alterations in canine FRE. The findings align with mechanisms described in human inflammatory bowel disease and support further investigation of bile acid signaling in canine chronic enteropathy. Findings should be interpreted as exploratory due to cohort heterogeneity. - Source: PubMed
Publication date: 2026/06/11
Mózes BorbálaKiss GergelyFóthi ÁbelFerenczi SzilamérPsáder Roland - The parotoid gland secretion of the medicinal toad () is the primary source of Chansu, a valuable traditional medicine. However, the lack of understanding regarding the biological regulation of venom synthesis limits the optimization of farming practices and quality control. Here, we integrated histology, lipidomics, transcriptomics, and untargeted metabolomics to delineate the regulatory mechanisms governing venom accumulation and biosynthesis, aimed at guiding aquaculture management. Histological results revealed that the parotoid gland is a highly regionalized organ, where structural maturity is positively correlated with toxin storage capacity, providing a morphological basis for determining optimal harvest timing. This structure is underpinned by a distinct lipid profile: high levels of triglycerides supply essential energy for massive venom production, while enriched ceramides and sphingomyelin form a specialized barrier to ensure high-quality venom encapsulation. Transcriptome analysis indicated that the upregulation of steroid and primary bile acid biosynthesis genes (e.g., , , , , and ) drives the synthesis of bioactive bufadienolides. Furthermore, we identified key enzymatic regulators─including CYP450s (, , ) and acetylases (, )─as potential genetic markers for identifying high-yielding venom. Collectively, these findings provide a scientific basis for establishing standardized farming protocols, advancing molecular breeding strategies for this important medicinal species, and achieving sustainable exploitation of toad venom. - Source: PubMed
Publication date: 2026/06/18
Wang YaxiYu XiaopingPeng JufangWang Hongyuan