Ask about this productRelated genes to: CLPB Blocking Peptide
- Gene:
- CLPB NIH gene
- Name:
- ClpB homolog, mitochondrial AAA ATPase chaperonin
- Previous symbol:
- -
- Synonyms:
- HSP78, SKD3, FLJ13152, ANKCLB
- Chromosome:
- 11q13.4
- Locus Type:
- gene with protein product
- Date approved:
- 2005-10-04
- Date modifiied:
- 2019-04-23
Related products to: CLPB Blocking Peptide
Related articles to: CLPB Blocking Peptide
- Demodex mites inhabit the pilosebaceous unit despite harsh environmental conditions including UV radiation, variable salinity, and cosmetics. Their recently characterized endobacterium may contribute to this resilience. This study aimed to elucidate mechanisms of the microbe-host interaction that help mites withstand environmental stress. The genome of Corynebacterium kroppenstedtii subsp. demodicis was sequenced using PacBio technology and annotated via MicroScope. Metabolic and symbiotic traits were analyzed using KEGG and compared with the Demodex folliculorum secretome from published transcriptome data. The complete 2,456,075 bp genome contains 2034 coding sequences and exhibits reduced variable genes compared to other Corynebacterium species. Primary metabolism comprises an almost complete minimal gene set but lacks two tRNA synthetases and genes for phosphatidylethanolamine and NAD biosynthesis. Carbohydrate pathways are incomplete and fatty acid synthase I is absent. Secondary metabolism includes complete mevalonate and β-carotene biosynthetic pathways, while the methylerythritol phosphate pathway is missing. UV protection and oxidative stress tolerance are supported by β-carotene, ClpB, RecN, MsrA, KatA, SodA, and manganese transporter SitB. The secretome contains hydrolases likely aiding mite digestion. These findings provide genomic insights into mite-bacterium symbiosis and follicular adaptation. All functional inferences are based on genomic data and in silico predictions; experimental validation remains to be established. - Source: PubMed
Steegmüller TWalch SGschwendtner SKlingl AFrench L EFlaig MClanner-Engelshofen B M - The multicellular metazoan lineage acquired a novel chaperone in the mitochondrial intermembrane space, the AAA+ disaggregase and refoldase CLPB. Although it is not known how they function together, CLPB and the intrinsically disordered IMS protein HAX1 interact and share disease and cellular phenotypes; loss of function in either gene causes severe congenital neutropenia as well as neuropathology and causes many proteins in the IMS and its bounding membranes to become insoluble. We sought to determine how HAX1 functions with CLPB. Through biochemical reconstitution, we find that HAX1 is a stimulatory cofactor of CLPB. HAX1 promotes oligomerization of CLPB into an active disaggregase and stimulates the ATPase and refoldase activities of the oligomeric complex. A short peptide within HAX1 is necessary for direct interaction with the ankyrin domain of CLPB, but stimulation of CLPB activity requires additional elements of HAX1. Characterization of CLPB and CLPB-HAX1 oligomers indicates that HAX1 shifts the predominant oligomeric state of CLPB from a dodecamer to a hexamer elaborated 1:1 with HAX1, suggesting that this smaller oligomer is important during the cycle of CLPB function with clients. - Source: PubMed
Publication date: 2026/05/26
Fahie Monifa A VHoffman Julia GKardon Julia R - is an important food-borne pathogen which is frequently linked to ready-to-eat vegetables. In this work, the aim was to investigate by RNAseq how the colonization of corn salad leaves and the commonly used packaging material polystyrene impacts on the transcriptome of NCTC 10887 at 4 °C and 22 °C. The strongest transcriptional differences occurred on corn salad at 4 °C versus polystyrene at 4 °C, yielding 2629 differentially expressed genes (91.6% of protein-coding genes), indicating a habitat driven shift under cold-chain conditions. Corn salad promoted regulation of virulence related genes. The gene was upregulated on corn salad compared to polystyrene at both temperatures (log2FC 2.43 at 22 °C and 4.16 at 4 °C), accompanied by increased (2.70), (4.46), and (2.10) expression at 4 °C. Stress/proteostasis gene expression was elevated on corn salad, including higher transcripts of (3.34-3.51), (5.58-6.89), and (2.08-3.74), consistent with a stressed yet persistence-prone state on leaf surfaces. This indicates that persisting on leafy greens can remain transcriptionally primed for host interaction at 4 °C, implying that refrigeration alone may not mitigate risk on ready-to-eat produce, underlining the importance of pre-harvest hygiene. - Source: PubMed
Publication date: 2026/05/22
Hoffmann TimWeiss Agnes - Pseudomonas aeruginosa biofilms drive chronic infections, yet heterogeneous matrix composition hinders standardized in vitro classification of clinical isolates. This study aimed to develop a reproducible biofilm formation protocol using a defined supplement mix and elucidate proteomic signatures across biofilm categories to enable risk stratification and targeted therapies. - Source: PubMed
Publication date: 2026/05/08
Rain ZinnuSingh Ashish KumarKumari ShabnamKumar DeepakMaurya Ganesh KumarKumar RajneeshPrakash Pradyot - Lacritin is a tear, saliva, plasma and cerebrospinal fluid glycoprotein with broad polypharmacology. Selective deficiency of its bioactive monomeric form appears to be deleterious for ocular surface health for which replacement therapy is beneficial. Its cleavage-potentiated C-terminus represented by the N-104 proteoform in tears is bactericidal and synergizes with the tear thrombin peptide GKY20. In the pathogenic and multidrug resistant PA14 strain of , we recently discovered that N-104 binds to the outer-membrane lipoprotein YaiW to gain access to the periplasm where it targets and inhibits the inner-membrane ferrous iron transporter FeoB (of FeoABC) as well as PotH, a subunit of the polyamine transporter PotFGHI. Further, PA14 gene expression shifts toward anaerobic respiratory pathways. Here we explore N-104-associated transcriptional changes over a broader range of functional categories pointing to a reduction in: (i) virulence by suppressed gene expression of virulence factors AprA and LasA; and Hcp1 and PsrA necessary for the respective assembly of type VI and III secretion systems, (ii) fitness (less AtsC, MgtA), (iii) metabolism (less AdhA, AtsC, GcvH2, GcvP2, FadE1, SsuD, SsuF, TauB, TauD, UspK, UspN), (iv) stress response (less UnG, PfpI, RmF), (v) proteostasis (less ClpB, GrpE, HtpG), (vii) quorum sensing (less CifR, GcvH2, GcvP2, PsrA, QuiP), and (viii) survival under anaerobic conditions (less AdhA, MhR, ModA, UspKLNO). Upregulated genes are directed towards enhancing PA14: (i) multidrug (more OprJ of MexCD-OprJ) and (ii) tellurite (more TerC) efflux, coupled with a seemingly PA14 survival attempt at (iii) anaerobic respiration (more NosR), (iv) translational fidelity (more QueE, RimP, TrmD) and (v) metabolism (CysT, MoaA1, Sbp, SsuA, SsuE). The overlap with aminoglycosides (4.3%), β-lactams (0%), cyclic peptides (2.5%), fluoroquinilones (0%) and macrolide (1.9%) classes of antibiotics in was minimal. Thus, N-104 appears to widely perturb PA14 fundamental processes in a distinctive manner. - Source: PubMed
Publication date: 2026/03/18
Mohammad Sharifian GhFatemeh NorouziGordon W Laurie