Ask about this productRelated genes to: PRSS35 Blocking Peptide
- Gene:
- PRSS35 NIH gene
- Name:
- serine protease 35
- Previous symbol:
- C6orf158
- Synonyms:
- MGC46520, dJ223E3.1
- Chromosome:
- 6q14.2
- Locus Type:
- gene with protein product
- Date approved:
- 2003-06-11
- Date modifiied:
- 2018-01-19
Related products to: PRSS35 Blocking Peptide
Related articles to: PRSS35 Blocking Peptide
- Cleft palate only (CPO) is a multifactorial craniofacial malformation with significant genetic and epigenetic contributions. Among these, microRNAs (miRNAs) have emerged as key regulators of palate development, although their alterations in CPO remain incompletely characterized. In this study, we performed a comprehensive miRNA expression analysis on palatal tissues from an Italian cohort of non-syndromic CPO patients, compared with a human embryonic palatal mesenchymal (HEPM) cell line. Using the NanoString nCounter platform for miRNA profiling, we identified significant deregulation of several miRNAs, notably the upregulation of miR-205-5p and miR-200c-3p and the downregulation of miR-125a-5p in CPO tissues. Based on these expression changes, a functional analysis was carried out to identify potential target genes. Validation in primary cell cultures derived from patient tissues confirmed these expression patterns. Functional analyses and target predictions implicated PAX9, a key transcription factor essential for palatogenesis, as a probable target of miR-205-5p, while miR-125a-5p was associated with the regulation of PRTG and PRSS35-genes involved in neural crest cell biology and extracellular matrix remodeling, respectively. Although modulation of certain predicted targets of miR-200c-3p was observed, in vitro inhibition experiments did not show significant changes in gene expression, highlighting the complexity of miRNA regulatory networks and the need for further studies to unravel these interactions. These findings identify miRNA alterations associated with CPO tissue and fibroblasts, highlighting novel candidate pathways for further mechanistic and therapeutic investigation. - Source: PubMed
Publication date: 2026/02/24
Palmieri AnnalisaScapoli LucaPellati AgneseApolloni FedericoZanchi ValerioSpinelli GiuseppeSgarzani RossellaCarinci FrancescoMartinelli Marcella - As a major producer and consumer of duck meat, China faces industry challenges due to low feed conversion efficiency. Residual feed intake (RFI), a key metric for poultry feed utilization, remains poorly characterized in small-sized meat ducks. We raised 1,000 ducklings with similar initial body weight (BW) under controlled conditions until 63 days of age. RFI was calculated using average daily gain (ADG), average daily feed intake (ADFI), and metabolic body weight (MBW). Thirty high-RFI (HRFI) and thirty low-RFI (LRFI) ducks were selected to evaluate growth performance. Hypothalamic samples from 6 HRFI and 6 LRFI ducks underwent transcriptomic analysis, including differential gene expression, gene ontology, Kyoto Encyclopedia of Genes and Genomes pathway analysis, weighted gene co-expression network analysis, and miRNA target prediction. Results showed that the LRFI group had significantly lower feed intake (FI) and ADFI than the HRFI group (P < 0.05). Compared to low RFI controls, HRFI meat ducks exhibited 45 differentially expressed (DE) miRNAs (6 upregulated and 39 downregulated) and 323 DE mRNAs (133 upregulated and 190 downregulated), enriched in substance and energy metabolism pathways. Weighted gene co-expression network analysis identified ten hub DE miRNAs, including miR-1-3p, miR-10-5p/3p, miR-182-5p/3p, miR-183-5p, miR-263-5p, miR-96-5p, miR-7, and novel-m0108-5p. miRNA-mRNA network analysis revealed 43 DE regulatory pairs, including 15 with negative feedback. Notably, miR-182 targeted and regulated the highest number of DE mRNAs, showing negative feedback interactions with DDC, UPP2, PRSS35, and SLCO1C1. Dual-luciferase reporter assays confirmed the binding of partial genes. Given DDC's role in dopamine and serotonin synthesis, we further validated the miR-182-5p/DDC regulatory relationship through overexpression, interference, and Western blot experiments. This study provides novel insights into the molecular mechanisms underlying feed efficiency in ducks. - Source: PubMed
Publication date: 2025/03/21
Geng DandanYuan ChunyouLi XiaofanWang ChenxiaoGuo QixinJiang YongWang ZhixiuChen GuohongChang GuobinBai Hao - Rheumatoid arthritis-associated interstitial lung disease (RA-ILD) is a common complication of rheumatoid arthritis (RA) that result in significant morbidity and mortality. Understanding the molecular mechanisms underlying RA-ILD is crucial for effective prevention. This study aims to identify the specific molecule that mediate the causal association between RA and ILD, as well as to explore its potential mechanisms in the pathogenesis of RA-ILD. - Source: PubMed
Publication date: 2024/09/10
Liu MuqiuJiang ZhihaoLiu MinNi HaojieLi YanwuFang JiansongDu QunDong Yan - Mechanical deformation of skin creates variations in fluid chemical potential, leading to local changes in hydrostatic and osmotic pressure, whose effects on mechanobiology remain poorly understood. To study these effects, we investigate the specific influences of hydrostatic and osmotic pressure on primary human dermal fibroblasts in three-dimensional hydrogel culture models. Cyclic hydrostatic pressure and hyperosmotic stress enhanced the percentage of cells expressing the proliferation marker Ki67 in both collagen and PEG-based hydrogels. Osmotic pressure also activated the p38 MAPK stress response pathway and increased the expression of the osmoresponsive genes PRSS35 and NFAT5. When cells were cultured in two-dimension (2D), no change in proliferation was observed with either hydrostatic or osmotic pressure. Furthermore, basal, and osmotic pressure-induced expression of osmoresponsive genes differed in 2D culture versus 3D hydrogels, highlighting the role of dimensionality in skin cell mechanotransduction and stressing the importance of 3D tissue-like models that better replicate in vivo conditions. Overall, these results indicate that fluid chemical potential changes affect dermal fibroblast mechanobiology, which has implications for skin function and for tissue regeneration strategies. - Source: PubMed
Publication date: 2024/06/28
Garau Paganella LorenzaBadolato AsiaLabouesse CélineFischer GabrielSänger Catharina SKourouklis AndreasGiampietro CostanzaWerner SabineMazza EdoardoTibbitt Mark W - To explore the regulatory effect of human epididymis protein 4 (HE4) on renal fibrosis in mice with lupus nephritis (LN) and the underlying mechanism. Ten-week old MRL/LPR mice were injected with HE4 shRNA adenovirus vector through the renal pelvis for 5 days. Renal tissues were extracted for HE and Masson staining to evaluate pathological changes and fibrosis in lupus nephritis mice. The level of urine protein was measured using a biochemical analyzer, while the expression level of HE4 and p-NF-κB p65 in renal tissues was visualized using an immunofluorescence assay. The level of β2-microglobulin (β2-MG), neutrophil gelatinase-associated lipocalin (NGAL), and kidney injury molecule 1 (Kim-1) was determined by the immunohistochemical assay. Western blotting was used to determine the levels of C3, HE4, matrix metalloproteinase-2 (MMP2), MMP9, p-p65, prss23, and prss35 in renal tissues. Compared to wild-type C57BL/6 mice, MRL/LPR mice showed a marked increase in the number of glomeruli, hyperplasic basement membrane, severe infiltration of inflammatory cells in renal tubules and glomeruli, obvious necrosis in glomeruli, elevated fibrosis levels, and increased levels of urine protein, β2-MG, NGAL, Kim-1, C3, HE4, MMP2, MMP9, and p-p65; and decreased levels of prss23 and prss35 were observed in MRL/LPR mice. After the administration of the HE4 shRNA adenovirus vector, the repaired structure of renal tubules and glomeruli improved infiltration of inflammatory cells, reduced collagen fiber and urine protein, suppressed levels of C3, HE4, MMP2, MMP9, and p-P65, and facilitated the expression of prss23 and prss35 which were observed. Silencing HE4 improved renal fibrosis and inhibited inflammation in mice with lupus nephritis, which may play a role in inhibiting C3/MMPs and promoting prss-related protein expression. - Source: PubMed
Publication date: 2023/12/29
Li YixiaZhong XiaorongYang Feng