Ask about this productRelated genes to: RPLP0 Blocking Peptide
- Gene:
- RPLP0 NIH gene
- Name:
- ribosomal protein lateral stalk subunit P0
- Previous symbol:
- -
- Synonyms:
- PRLP0, P0, L10E, RPP0, LP0
- Chromosome:
- 12q24.23
- Locus Type:
- gene with protein product
- Date approved:
- 1993-12-16
- Date modifiied:
- 2016-10-05
Related products to: RPLP0 Blocking Peptide
Related articles to: RPLP0 Blocking Peptide
- The present study evaluated the stability of candidate reference genes during adipogenic differentiation of 3T3-L1 cells cultured on different extracellular matrices. The aim was to investigate the effects of matrix composition and differentiation stage on the expression of candidate housekeeping genes and to compare validation strategies in dynamic in vitro models. Eleven candidate reference genes (, , , , , , , , , , and ) were analyzed by RT-qPCR in 3T3-L1 cells cultured on TC, collagen, gelatin, and Matrigel at Days 7 and 14 of differentiation. Gene stability was assessed using geNorm, NormFinder, RefFinder, comparative ΔCt, BestKeeper, generalized linear model (GLM), linear mixed model (LMM), and correlation analyses with the adipogenic markers and . The results demonstrated that the expression of most housekeeping genes was influenced by matrix composition, differentiation stage, or their interaction. and exhibited the strongest condition-dependent variability and pronounced matrix sensitivity. and showed significant correlations with both and , while correlated with , suggesting that these reference genes may not be fully independent of adipogenic status. demonstrated markedly contrasting rankings across analytical approaches, highlighting limitations of single-method stability assessment. The findings confirm that universal housekeeping genes are unlikely to exist across different matrix conditions and differentiation stages. The results highlight the need for multi-level validation strategies and experimentally validated normalization panels to minimize normalization bias and avoid misleading RT-qPCR expression profiles. Functional validation identified and as the most suitable two-gene normalization panel for the experimental model evaluated, whereas remained a strong complementary reference gene candidate. - Source: PubMed
Publication date: 2026/06/10
Todorova BetinaIvanova ZhenyaGrigorova Natalia - Sepsis is a life-threatening, heterogeneous syndrome with high mortality. This heterogeneity undermines current "one-size-fits-all" therapies and conventional biomarkers (e.g., PCT) lack prognostic power. A critical need exists to identify patient-specific endotypes and causal-driven therapeutic targets. - Source: PubMed
Publication date: 2026/05/28
Zhou JunLiu YunHu QiuyanXu ChengzhiYin FeiLu YeShi Qiang - The ribosome is a ribozyme, but it also acts as a dynamic regulator of gene expression. Although ribosomal protein (RP) composition varies, dissecting the functional contributions of individual RPs beyond their housekeeping roles is challenging because of the lack of tools for manipulation in situ. Here, we developed Ribo-Tweezer, a degron-based system directly tethered to mature ribosomes that enables rapid, reversible, and selective depletion of specific RPs. Using Ribo-Tweezer in mouse embryonic stem cells (mESC), we find a previously uncharacterized role for RACK1 in stem cell fate control via translational regulation of zinc-finger transcriptional networks and long interspersed nuclear element-1 (LINE1) expression. This translation-transcription coupling provides a mechanism by which translation control is further amplified in gene regulation. Distinct translational programs induced by RPLP0 and RPLP1 depletion further demonstrate RP-specific regulatory functions in translation. Together, these findings establish Ribo-Tweezer as a powerful platform that has illuminated selective functions for RPs in gene regulation, which gives biological meaning to ribosome heterogeneity. - Source: PubMed
Publication date: 2026/05/21
Chen YuxiangCheng Ching PinCates KitraMarinov Georgi KLantz Travis CYang HaojunLiu IsabelleGenuth Naomi RAndronescu ChristinaHung VictoriaBermudez AbelRothschild DaphnaGeorgeson JosephBarocio Sonia BustosKundaje AnshulPitteri SharonRuggero DavideBarna Maria - Reverse transcription quantitative real-time polymerase chain reaction is the gold standard for gene expression quantification. Yet, this method's accuracy heavily depends on choosing appropriate reference genes for data normalization. Reference genes must display stable expression levels across biological and experimental conditions to ensure accurate and meaningful results. - Source: PubMed
Publication date: 2026/04/24
Mengi Camur NazSeker BusranurKizildag FulyaYanik TulinAdams Michelle M - Neuroinflammation, particularly that involving reactive microglia, the brain's resident immune cells, is implicated in the pathogenesis of major neurodegenerative diseases (NDs). Multiple studies have reported changes in ribosomal protein (RP) expression during neurodegeneration, but the significance of these changes remains unclear. Ribosomes are evolutionarily conserved protein-synthesizing machines, and although commonly viewed as invariant, accumulating evidence suggests functional ribosome specialization through variation in their protein composition. Among RPs, S24, encoded by RPS24 in humans and Rps24 in mice, is unique as its transcripts undergo alternative splicing to produce protein variants with different C-terminal sequences that are differentially expressed across tissues and cell types. Understanding heterogeneous RP expression patterns across brain regions and cell types could reveal mechanisms underlying selective vulnerability in NDs and provide new biomarkers for neuroinflammatory responses. To identify RP expression patterns across brain regions in neurons, astrocytes, and microglia we analyzed cell type-specific translating mRNAs from mice. To investigate Rps24 isoform-specific expression, we performed cell type-resolved transcript analysis and developed antibodies specific for the S24-PKE protein variant encoded by mRNA isoform Rps24c. We examined Rps24c/S24-PKE expression in brains from mouse models of aging and neurodegeneration, as well as in human postmortem tissue from patients with Alzheimer's disease (AD), Parkinson's disease (PD), and Huntington's disease (HD). This work revealed distinct RP expression patterns across brain regions and between neurons, astrocytes, and microglia, including neuron-enriched RPs Rpl13a and Rps10. Analysis of RP paralogs revealed complex expression relationships with their canonical counterparts, suggesting regulated mechanisms for generating heterogeneous ribosomes. Across brain regions and cell types, Rplp0 and Rpl13a, commonly used normalization references, showed heterogeneous expression, raising important methodological considerations for gene expression studies. Rps24 isoforms exhibited striking cell type-specific expression patterns. Rps24c was predominantly expressed in microglia and was increased by neuroinflammation caused by aging, neurodegeneration, or inflammatory chemicals. Using S24-PKE-specific antibodies, we verified increased expression of this protein variant in brains with AD, PD, and HD, and in relevant mouse models. These findings establish heterogeneous RP expression as a feature of brain cell types which may enable cell type-specific translation regulation via specialized ribosomes. This work also identifies Rps24c/S24-PKE as a potential novel marker for neuroinflammation and neurodegeneration and provides new tools for monitoring these responses. - Source: PubMed
Publication date: 2026/05/06
Magadi Srivathsa SJonson MariaLucena Pablo BCaliandro Michele FAlmeida BeatrizBilalli LorinaBudinger DimitriTsoi AnnaNtzouni MariaMaqdissi Joseph AgiKaczmarczyk LechZijlstra Jente JFaketija MatejPerkins MatthewPaul GesineHallbeck MartinIngelsson MartinWatts Joel CReichenbach NicolePetzold Gabor CSchieweck RicoHeneka Michael TJackson Walker S