Ask about this productRelated genes to: FZD8 Blocking Peptide
- Gene:
- FZD8 NIH gene
- Name:
- frizzled class receptor 8
- Previous symbol:
- -
- Synonyms:
- -
- Chromosome:
- 10p11.21
- Locus Type:
- gene with protein product
- Date approved:
- 1998-09-17
- Date modifiied:
- 2016-10-05
Related products to: FZD8 Blocking Peptide
Related articles to: FZD8 Blocking Peptide
- Hsa-miR-99a has been linked to the advancement of several malignancies, including colorectal cancer (CRC). This investigation seeks to elucidate its function and regulatory network in CRC. - Source: PubMed
Publication date: 2026/03/17
Yang XuejingZhang TingtingSun HuFeng HuijingSong Dong - Wnt ligands stimulate β-catenin-dependent (canonical) or β-catenin-independent (noncanonical) signaling, depending on which co-receptors are recruited to the Wnt receptor FZD. Both pathways are initiated by receptor oligomerization into signalosomes and involve a largely overlapping set of downstream effectors. To resolve the assembly of Wnt signalosomes with high spatiotemporal resolution for extended times, we developed single-molecule tracking and localization microscopy based on labeling with reversibly binding nanobodies (rbTALM). We engineered nanobody-tag pairs with finely tuned binding affinities to ensure single-molecule tracking with high fidelity while also permitting continuous exchange of photobleached labels. Multicolor rbTALM imaging enabled simultaneous tracking and super-resolution imaging of three different tagged Wnt co-receptors in the same cell for more than 1 hour at video rate. Time-lapse correlation analyses uncovered cooperative association of canonical (LRP6) and noncanonical (ROR2) Wnt co-receptors with FZD8 into a common, hybrid Wnt signalosome. These findings demonstrate the potential for rbTALM imaging for exploring nanoscale dynamics across millisecond to hour timescales and for deciphering the molecular dynamics that underlie signaling complex formation. - Source: PubMed
Publication date: 2026/03/03
Philippi MichaelDohle JuliaWatrinet IsabelleHoltmannspötter MichaelMiao YiLi JinyeBirkholz OliverRothbauer UlrichGarcia K ChristopherKurre RainerPiehler JacobYou Changjiang - FZD8, a GPCR, is involved in various physiological processes, such as cell differentiation, bone growth and stem cell regulation, by binding to various Wnt ligands. Though FZD receptors have low mutation rates in cancer, aberrant Wnt or FZD expression leads to deregulated Wnt/FZD signaling pathways that may result in tumorigenesis. The structural and functional effects of most of the coding and non-coding SNPs of human FZD8 gene are yet to explore. In the present study, we analyzed 450, 109, and 390 SNPs in the CDS, 3'UTR, and 5'UTR of the FZD8 gene, respectively, using advanced state-of-the-art bioinformatics tools to explore their structural and functional consequences in tumorigenesis. We identified 10 highly deleterious nsSNPs among which 6 nsSNPs were located within the Wnt1 binding CRD region of FZD8. Additionally, these nsSNPs were also predicted to affect post-translational modifications in FZD8. The highly deleterious variant P120Q, causes significant structural change in secondary structure of FZD8 mRNA. Structure based stability prediction revealed 4 destabilizing variants among 6 highly deleterious variants. Wnt1 binds most strongly with FZD8-CRD among other FZD-CRDs. MD simulation analyses revealed that the binding energies of the mutated complexes were less stabilizing compared to the wild-type Wnt1-FZD8-CRD complex, with the P74L and A119E complexes predicted to be the most destabilizing. SNPs, rs1408188233 (34.T > C) in 3'UTR and rs1588706985 in 5'UTR may lead to AGO2 mediated and E2F6 mediated silencing of the FZD8 gene, respectively, whereas nsSNPs-rs1322411573 and rs1410965895 in the CDS may abolish miRNA-mediated gene silencing. Differential expression of FZD8 was observed across various normal, tumor, and metastatic tissues, and its deregulation was associated with reduced survival outcomes in patients with different types of cancer. Additionally, several biomarkers involving FZD8 mutations have been identified in patients with gastric cancer, multiple myeloma, and uterine cancer. Furthermore, these computationally prioritized high risk SNPs of FZD8 can be investigated in population based genetic studies and may serve as potential targets for future drug development against FZD8-associated diseases. - Source: PubMed
Publication date: 2026/02/24
Mondal AmaleshPaul DebaratiMondal TithiGoswami Achintya Mohan - Cancer immunotherapy with immune checkpoint inhibitors (ICIs) is often limited by an immunosuppressive tumor microenvironment (TME). Simultaneous targeting of the TME and immune checkpoints is a promising approach to address this limitation. Here we develop an inhalable exosome system that enables co-display of two inhibitory ligands and apply it to treat lung metastases of ICI-resistant melanoma. As immune exclusion in this context is often mediated by Wnt/β-catenin signaling, we harnessed the Alix sorting domain for tandem display of PD-1 and FZD8 to block PD-L1 and Wnt7b, which is overexpressed in ICI-resistant melanoma. This technology, called bispecific exosome activator of T cells (BEAT), enables uniform 1:1 co-display of two proteins on the exosome surface. We show that BEAT concurrently recruits and activates CD8⁺ T cells to reprogram the TME, yielding robust antitumor activity in ICI-resistant melanoma mouse models. Inhaled BEAT outperforms linked dual antibody targeting PD-L1 and Wnt7b in vivo. This approach to tandem protein display may be applicable to diverse ICI-resistant cancers. - Source: PubMed
Publication date: 2026/01/05
Liu ShuoLiu MengruiWang ZhenzhenHu ShiqiZhang KaiyueLu ChaoCheng XiaoShen MingBi JianingZhu DashuaiCheng Ke - Prostate adenocarcinoma is mainly diagnosed based on serum PSA levels, but elevated PSA levels can also be caused by BPH, which weakens its specificity. Recent scientific studies have demonstrated that specific microRNAs regulate cancer cell proliferation by modulating the Wnt/β-catenin pathway. To date, no published literature has provided a comprehensive assessment of the interactions between miR-106a-5p and miR-375-3p and components of the Wnt/β-catenin pathway in prostate cancer. Therefore, the aim of the present study was to perform a pilot evaluation of the expression of miRNAs 106a-5p and 375-3p, as well as β-catenin, Fzd8, Wnt5a, and cyclin D1 in prostate adenocarcinoma compared with BPH. The study material consisted of samples collected from 30 patients with prostate cancer and 30 with BPH. Protein expression was analyzed using IHC and qRT-PCR methods, while miRNA levels were quantified by dPCR. Our study results revealed lower immunoreactivity and expression of genes encoding β-catenin, Fzd8, Wnt5a, and cyclin D1 and significantly higher fluorescence intensity of miRNA 106a-5p and 375-3p with prostate adenocarcinoma compared to BPH. These parallel alterations in miRNA expression and Wnt/β-catenin-related components reflect disease-specific expression patterns and warrant further investigation in larger cohorts to determine their potential utility as diagnostic biomarkers in prostate diseases. - Source: PubMed
Publication date: 2025/12/15
Smereczańska MagdalenaDomian NataliaMłynarczyk GrzegorzKasacka Irena