VMD2L2 Blocking Peptide
- Known as:
- VMD2L2 Blocking Peptide
- Catalog number:
- 33r-6572
- Product Quantity:
- USD
- Category:
- -
- Supplier:
- Fitzgerald industries international
- Gene target:
- VMD2L2 Blocking Peptide
Related genes to: VMD2L2 Blocking Peptide
- Gene:
- BEST4 NIH gene
- Name:
- bestrophin 4
- Previous symbol:
- VMD2L2
- Synonyms:
- -
- Chromosome:
- 1p34.1
- Locus Type:
- gene with protein product
- Date approved:
- 2003-12-03
- Date modifiied:
- 2015-01-28
Related products to: VMD2L2 Blocking Peptide
Related articles to: VMD2L2 Blocking Peptide
- Specialist intestinal epithelial cells are critical for barrier integrity and immune responses at the mucosal boundary, yet the pathways that govern their development are incompletely defined. Here, we identify an essential role for TNFRSF11A/RANK in shaping intestinal epithelial specialization in zebrafish. Using lineage trajectory analysis, we identify two tuft cell subtypes, including a subtype enriched for expression of genes required to produce pro-inflammatory leukotrienes. We show that RANK deficiency reduces the abundance of immune-regulatory tuft and BEST4 cells, increases goblet cell frequency, and promotes the accumulation of pro-inflammatory leukocytes in the gut. Functionally, we demonstrate that the number of cells expressing the BEST4 cell marker cftr expand following infection with a pandemic strain of Vibrio cholerae, and we show that RANK deficiency enhances fish susceptibility to host colonization by Vibrio, implicating this lineage in host defenses against an enteric pathogen. Together, our findings implicate RANK signaling in intestinal epithelial diversification and immune regulation. - Source: PubMed
Publication date: 2026/05/19
Willms Reegan JMcCabe ToriJones Lena OcampoSchade RuthFoley Edan - To generate a single-cell atlas of colorectal cancer (CRC) development in Lynch syndrome (LS), and to delineate the associated cellular reprogramming and intercellular communication networks. - Source: PubMed
Chen ShangxiangFeng JishenFang ZhengkaiJiang YuhaoDuanmu JinzhongYu XinWang JunfuJiang Qunguang - Surrogate endpoints have been proposed to accelerate the evaluation of cancer screening but are controversial. Recent systematic reviews of advanced stage and cancer mortality in randomised trials of cancer screening have reached different conclusions regarding the suitability of advanced stage as a surrogate endpoint. We aimed to consider the suitability of trial-level meta-analysis to evaluate surrogacy and propose an alternative practical framework to evaluate potential surrogate endpoints for cancer screening trials. - Source: PubMed
Publication date: 2026/04/01
Sasieni Peter DBrentnall Adam RDuffy Stephen W - Faithfully recapitulating the cellular heterogeneity of the intestinal epithelium is essential when using organoid models. Air-liquid interface (ALI) culture has been shown to promote secretory cell differentiation, but its impact on gene expression in each epithelial cell type remains unclear. In this study, we used single-cell RNA sequencing (scRNA-seq) to characterize the cellular heterogeneity of rabbit cecum-derived organoid monolayers grown under immerged or ALI conditions. We then compared these organoid cell type-specific gene expression profiles to a scRNA-seq atlas of the rabbit cecal epithelium in vivo. We selected the rabbit model notably because, unlike mice, it possesses BEST4 epithelial cells, a newly discovered subset of mature absorptive cells. Our analysis revealed a high degree of transcriptomic similarity between in vivo and organoid-derived stem and transit-amplifying cells. ALI culture markedly enhanced the differentiation of the secretory lineage, especially goblet cells, whose transcriptome closely resembled that of in vivo goblet cells. Furthermore, ALI was the only condition allowing the detection of enteroendocrine cells. BEST4 cells, however, were absent from organoids in immerged or ALI conditions despite their presence in vivo. In addition, ALI culture led to a consistent downregulation of hypoxia and glycolysis-associated genes across all cell types, which suggests a metabolic shift likely driven by increased oxygen availability in ALI conditions. Cell-cell communication analyses further indicated that ALI more closely mirrored in vivo patterns than immerged condition. Altogether, these results demonstrate that ALI culture allows for better recapitulation of the in vivo cellular heterogeneity and molecular signatures of the intestinal epithelium. Using single-cell RNA sequencing, this study shows that air-liquid interface (ALI) culture enhances secretory lineage differentiation of intestinal organoid cell monolayers and improves transcriptomic similarity to the native epithelium. ALI reduced hypoxia-associated gene expression and better recapitulates in vivo-like cell-cell interactions, supporting its value for modeling intestinal epithelial heterogeneity in organoids. - Source: PubMed
Publication date: 2026/03/14
Malonga TaniaLhuillier EmelineMarrauld ChristelleFourmy DeborahRiant ElodieCabau CedricVialaneix NathalieBeaumont Martin - To mine single-cell sequencing data for colorectal cancer (CRC), identify CRC epithelial cell subtypes, and explore the heterogeneity of epithelial cells and their impact on the tumor microenvironment (TME). The GSE201348 dataset, including normal, colorectal adenoma, high-grade colorectal intraepithelial neoplasia, and CRC tumor tissue samples, was downloaded from the Gene Expression Omnibus. The Seurat package of R software was used for data quality control, data integration, normalization, and clustering. The Feature Plot and the Recode function were executed to annotate and group the epithelial cells. Finally, genetic differences, copy number variant heterogeneity, pseudotime, cell-cell communication, and Gene Set Variation Analysis (GSVA) were further conducted. In total, 26,335 gene matrices from 263,872 cells were obtained for subsequent analyses. Four cell clusters, including immune cells, fibroblasts, endothelial cells, and epithelial cells, were identified. Epithelial cells were further divided into 11 subgroups characterized by MKI67, SLC27A6, PLCE1, NKD1, KCNMA1, GDA, CLCA4, BEST4, LRMP, ACTG2, and ASPM. GSVA enrichment analysis suggested a role of the "P53 pathway," "Wnt-β-catenin signaling," and "MYC targets V1" pathways in epithelial cells during the malignant progression of tumors. Survival analysis indicated that downregulation of KCNMA1 and upregulation of MKI67 were associated with poor prognosis. Cell-cell communication analysis suggested a bidirectional regulatory role between epithelial and fibroblast subsets. This study analyzed the gene expression characteristics of 11 types of epithelial cells during the malignant progression of CRC. KCNMA1 and MKI67 epithelial subpopulations are important indicators for the malignant progression of CRC. - Source: PubMed
Publication date: 2026/02/05
Chen QianqianYuan YaoqianTian ShuaiZhou JiayanLv KunmingLinghu Enqiang