Ask about this productRelated genes to: RAB5B Blocking Peptide
- Gene:
- RAB5B NIH gene
- Name:
- RAB5B, member RAS oncogene family
- Previous symbol:
- -
- Synonyms:
- -
- Chromosome:
- 12q13
- Locus Type:
- gene with protein product
- Date approved:
- 1994-08-30
- Date modifiied:
- 2015-08-25
Related products to: RAB5B Blocking Peptide
Related articles to: RAB5B Blocking Peptide
- Leishmania donovani Rab5 isoforms, LdRab5a and LdRab5b, play distinct roles in the early stages of endocytosis, with LdRab5a primarily regulating fluid-phase uptake and LdRab5b modulating receptor-mediated endocytosis. These functional differences are governed by structural variations within their GTPase domains. Here, we report the crystal structure of the GTPase domain of LdRab5a in its active, GppNHp-bound, form and compare it to the previously solved GDP-bound structure. Binding of the γ-phosphate analog induces a closed conformation of the Switch I and Switch II regions, a characteristic of the active state. Circular Dichroism and thermal stability assessments using Differential Scanning Calorimetry confirmed proper folding of the protein and revealed that guanine nucleotide and Mg binding, significantly enhanced protein stability. Furthermore, molecular dynamics simulations were conducted to explore the conformational dynamics of LdRab5a in both GDP and GppNHp-bound states. The GppNHp-bound form exhibited greater structural stability compared to the GDP-bound inactive form, with significantly reduced flexibility in the Switch I region. We also present the crystal structure of LdRab5b in its GDP-bound state. Structural analysis suggests that LdRab5b may adopt an intermediate conformation during nucleotide exchange, evidenced by weak Mg coordination and a flipped-out conformation of the Switch II residue Ala78. Comparative structural analysis with human Rab5b and Arabidopsis thaliana Ara7 reveals significant differences in helix α1, strand β2, and the positioning of the hydrophobic triad residues, indicating lineage-specific adaptations in LdRab5b. Collectively, our study provides novel insights into the structure-function relationships of Leishmania Rab5 isoforms and their differential roles in endocytic trafficking. - Source: PubMed
Publication date: 2026/05/01
Pandey DivyaZohib MuhammadChaurasia AnimeshAnsari HeraChowdhury AvishekPal Ravi KantTripathi SaritaJain AnupamBiswal Bichitra KumarSiddiqi Mohammad ImranArora Ashish - - Source: PubMed
Publication date: 2026/02/26
Acharya SrijanPatel Girijesh KumarKhan Mohammad AslamClark Amanda MSaranyutanon SirinAnand Shashi - Cynoglossus semilaevis, an important mariculture species in China, faces significant losses due to Vibrio infections. Current antibiotic treatments are constrained by efficacy and environmental considerations. While Rab5b is known to regulate phagosome maturation in immunity, its direct antimicrobial function and therapeutic potential have not been explored. This study characterized rab5b (651 bp ORF, 216 aa) through bioinformatics, expressed recombinant Rab5b prokaryotically, and evaluated its roles using antimicrobial assays, membrane integrity tests (FITC/PI, SEM, Crystal Violet Staining, TEM), transcriptomics (RNA-seq, WGCNA, STEM), and in vivo challenges. Rab5b exhibited widespread tissue expression, with significantly higher expression in the gut (approximately 8.5-fold) and blood (approximately 6.2-fold) compared to the liver (p < 0.05), and showed significant upregulation following V. vulnificus infection. Recombinant Rab5b demonstrated broad-spectrum activity against 8 pathogenic bacteria, such as Aeromonas spp. and V. vulnificus, inhibiting biofilms and disrupting membrane integrity through binding and permeabilization. Transcriptomic analysis revealed that Rab5b coordinates DNA repair-immune crosstalk by activating ATM/ATR pathways to upregulate FA/BER genes (fen1, pcna, rad51, etc.) and collaborates with AURKA for cell cycle progression. In vivo experiments demonstrated that Rab5b reduced mortality by 46.7% in infected fish and improved bacterial clearance. This study establishes Rab5b's dual role as an antimicrobial peptide and immunomodulator, offering a novel therapeutic approach against biofilm-associated infections and a host-directed strategy to address antibiotic resistance in aquaculture. - Source: PubMed
Publication date: 2026/03/12
Han TianLiu YufengHe ZhongweiZhang YitongCao WeiRen JiangongWang GuixingRen YuqinGong ChunguangHou Jilun - Vertebrate radial glia progenitors (RGPs) balance self-renewal and differentiation through asymmetric cell division (ACD), which involves unequal centrosome inheritance. How centrosome asymmetry directs cell fate remains poorly understood. Here, we identify Pericentriolar material 1 (Pcm1) as a key player in this process. In zebrafish embryonic RGPs, Pcm1 is asymmetrically associated with Cep83, a mother centrosome marker. Using in vivo time-lapse imaging and nanoscale-resolution expansion microscopy, we detect Pcm1 on Notch ligand-containing endosomes, where it interacts-either directly or indirectly-with Par-3 and dynein. Loss of pcm1 disrupts endosome dynamics, increasing neuronal differentiation at the expense of RGP self-renewal. Mechanistically, Pcm1 facilitates the transition from Rab5b to Rab11a and promotes the assembly of Par-3 and dynein macromolecular complexes on recycling endosomes. Furthermore, we find conserved PARD3-PCM1-CEP83-RAB11 associations in human cortical brain organoids. Our findings uncover that Pcm1 links centrosome asymmetry to polarized endosome trafficking, thereby regulating RGP fate decisions. - Source: PubMed
Publication date: 2025/11/28
Zhao XiangMouilleau VincentWang YiqiSolak Ahmet CanGarcia Jason QChen XinyeShi XiaoyuWilkinson Christopher JRoyer Loïc ADong ZhiqiangGuo Su - Polycystic ovary syndrome (PCOS) is a complex endocrine disorder affecting reproductive-aged women. Previous studies have identified genomic associations at chromosome 12q13.2, but the functional mechanisms underlying these associations remain unclear. We integrated three complementary datasets: (1) WES-identified single nucleotide variants (SNVs) from PCOS and normal theca cells with association testing for forskolin-stimulated androgen production, (2) STARR-seq enhancer activity data with eQTL colocalization analysis, and (3) scRNA-seq expression data comparing forskolin-stimulated PCOS and normal theca cells. We previously identified haplotypes involving 10 SNVs at 12q13.2 containing // that are significantly associated with forskolin-stimulated androgen production. The identified haplotypes were further shown to associate with PCOS in a whole genome sequencing (WGS) cohort. Other studies have recently found the enhancer variant rs1081975 demonstrated perfect colocalization (PP = 1.0) with // eQTLs. Our scRNA-seq analysis revealed differential expression patterns for key genes. showed a forskolin response upregulation in normal cells but an impaired response in PCOS. exhibited opposite forskolin responses between normal and PCOS cells. , an androgen corepressor in the locus, was upregulated in normal untreated cells. , an epidermal growth factor receptor in the locus, was downregulated in normal forskolin treated cells. The integration of multimodal genomic data provides functional validation of PCOS-associated variants at 12q13.2, revealing coordinated dysregulation of vesicular trafficking (), androgen receptor regulation (), and metabolic processes () in PCOS theca cells. - Source: PubMed
Publication date: 2025/11/19
Harris R AlanMcAllister Jan MStrauss Jerome F