Ask about this productRelated genes to: TNP1 Blocking Peptide
- Gene:
- TNP1 NIH gene
- Name:
- transition protein 1
- Previous symbol:
- -
- Synonyms:
- -
- Chromosome:
- 2q35
- Locus Type:
- gene with protein product
- Date approved:
- 1990-03-14
- Date modifiied:
- 2016-10-05
Related products to: TNP1 Blocking Peptide
Related articles to: TNP1 Blocking Peptide
- Testicular development and spermatogenesis are critical for male reproduction, but their molecular mechanisms in Dezhou donkeys remain understudied. This study used single-cell RNA sequencing (scRNA-seq) to analyze testicular tissues from Dezhou donkeys at juvenile (2 months), pre-pubertal (12 months), and mature (24 months) stages. A total of 24,606 high-quality cells were profiled, constructing a comprehensive single-cell transcriptional atlas. Unsupervised clustering identified nine major cell types: three germ cell subtypes (spermatogonia, spermatocytes, spermatids) and six somatic cell subtypes (Leydig cells, Sertoli cells, peritubular muscle cells, macrophages, endothelial cells, T cells). Key marker genes (AMH, TNP1, UTF1, ZMYND10) were validated by immunofluorescence. Pseudotemporal trajectory analysis revealed sequential germ cell differentiation (spermatogonia → spermatocytes → spermatids) and Sertoli cell maturation (immature → mature), while Leydig cells and peritubular muscle cells shared common progenitors. CellChat analysis identified critical ligand-receptor pairs in BMP, IGF, WNT, and FSH pathways, which regulate testicular development. This study provides the comprehensive single-cell transcriptional map of Dezhou donkey testicular development, elucidating key molecular mechanisms of germ and somatic cell maturation. The findings offer valuable insights into donkey reproductive biology, supporting breeding improvement and male infertility research. - Source: PubMed
Publication date: 2026/05/26
Wang ZhaofeiYu JieLiu GuiqinJafari HalimaLi CongYang GeLei ChuzhaoDang Ruihua - An epidemiological association between breast cancer (BC) and thyroid cancer (TC) has been found in several observational studies, but whether they share common genetic factors remain unknown. Our research aimed to investigate the shared genetic architecture and potential causal link between BC, including estrogen receptor-positive (ER+) and estrogen receptor-negative (ER-) subtypes, and TC. - Source: PubMed
Publication date: 2026/05/14
Yang QiaoyuTang HaiyunFan YuanjianLiu BaiyingZhang YunjianWu Jing - Spermiogenesis, the terminal phase of spermatogenesis, involves complex morphological and molecular changes essential for sperm maturation. While PRAMEL12, a cancer/testis antigen PRAME (preferentially expressed antigen of melanoma) family member, is implicated in spermatogenesis, its specific functions remain poorly understood. Here, global Pramel12 deletion in mice causes complete male infertility. Mutant males exhibit severe spermiogenesis defects, including impaired spermatid nuclear condensation, spermiation failure, and loss of sperm individualization. Consequently, sperm counts are drastically reduced, motility is severely impaired, and morphological abnormalities are markedly increased. Single-cell RNA-Seq reveals dysregulation of key genes governing sperm chromatin condensation and nuclear maturation in Pramel12-null spermatids. Proteomic profiling shows significant alterations in proteins essential for sperm structure, function, and manchette assembly. Critically, histochemical and protein profile analyses reveal a defective histone-to-protamine transition, characterized by aberrant histone modifications (including elevated H3K4me3, H3K9me3, H3K27me3, H3K23ac, and reduced H4K8ac), impaired TNP1/TNP2/PRM2 incorporation, and increased retention of core histones (H2A, H3, and H4) in mature sperm. Collectively, our findings establish PRAMEL12 as an essential regulator of spermiogenesis. Its deficiency disrupts histone-to-protamine exchange, causing abnormal sperm morphogenesis and functional defects that impair sperm quality and lead to male infertility. This identifies a novel mechanism for defective sperm chromatin condensation and male infertility. - Source: PubMed
Publication date: 2026/03/20
Li NanaWang XiaoLi HongWang Zhengpin - Tibetan sheep, a dominant livestock species on the Qinghai-Tibet Plateau, is characterized by late sexual maturity and low reproductive efficiency. Although long non-coding RNAs (lncRNAs) are known to play critical regulatory roles in mammalian testicular development and spermatogenesis, their expression dynamics and functions in Tibetan sheep remain poorly understood. In this study, we integrated histological and transcriptomic analyses to profile testicular lncRNAs across three developmental stages: pre-pubertal (3 months), sexually mature (1 year), and adult (3 years). Histological examination showed progressive structural maturation of seminiferous tubules, accompanied by significant increases in testicular weight and serum testosterone levels. RNA sequencing identified 10,857 high-confidence lncRNAs and uncovered extensive reprogramming of the lncRNA transcriptome during sexual maturation, with 7784 lncRNAs differentially expressed between pre-pubertal and post-pubertal stages. Functional enrichment analyses of cis- and antisense-target genes indicated that these lncRNAs were involved in key biological processes, including cell cycle regulation, TGF-β and Hippo signaling pathways, extracellular matrix organization, glycolysis, and apoptosis. Co-expression network analysis further linked upregulated lncRNAs to spermatogenesis-related genes involved in processes such as sperm nuclear condensation (e.g., TNP1) and metabolic support (e.g., PFKP). Our findings demonstrated that lncRNAs coordinate testicular development and spermatogenesis in Tibetan sheep by modulating transcriptional networks, remodeling the cellular microenvironment, and reprogramming energy metabolism. This study provides the first comprehensive atlas of testicular lncRNAs in Tibetan sheep and offers novel insights into the epigenetic regulation of male reproduction in high-altitude mammals. - Source: PubMed
Publication date: 2026/01/07
Li TaotaoWang HuihuiLuo RuiruiSong JuanjuanWu YiJia MengZhang YongMa Youji - Chronic occupational exposure to ionizing radiation (IR) may affect male reproductive health but the underlying genetic and immunologic mechanisms remain poorly defined. This study aimed to evaluate the effects of IR on semen quality, CD56-positive natural killer (NK) cell concentration and sperm nuclear protein gene expression. In this case-control study a total of 60 men were assessed 30 radiology workers with IR exposure and 30 men in the control group. Semen analysis was performed according to WHO 2021 criteria. NK cells were determined by CD56 immunocytochemical staining and mRNA expression levels of TNP1, TNP2 and CD56 were measured by qPCR. Compared with the control group the IR group had significantly lower sperm concentration and a higher frequency of morphological abnormalities (p < 0.05). NK cell concentration was significantly increased (p < 0.001) whereas TNP1 was significantly decreased (p < 0.001). TNP1 mRNA levels were downregulated in exposed subjects whereas TNP2 expression showed a non-significant reduction. Weak to moderate correlations were observed between gene expression profiles and selected sperm parameters. In conclusion chronic occupational IR exposure is associated with impaired semen quality, heightened NK cell concentration and altered nuclear protein gene expression, potentially compromising male reproductive health. These findings highlight the need for reproductive health monitoring and protective strategies among radiation-exposed healthcare workers. - Source: PubMed
Publication date: 2026/01/03
Aksak TiinçeÖnal Harika TopalOğuz İzzet