Ask about this productRelated genes to: H2AFY Blocking Peptide
- Gene:
- H2AFY NIH gene
- Name:
- H2A histone family member Y
- Previous symbol:
- -
- Synonyms:
- macroH2A1.2
- Chromosome:
- 5q31.1
- Locus Type:
- gene with protein product
- Date approved:
- 1999-07-22
- Date modifiied:
- 2015-11-18
Related products to: H2AFY Blocking Peptide
Related articles to: H2AFY Blocking Peptide
- Shank feathering (ptilopody) in chickens is primarily driven by cis-regulatory variants at the PITX1, TBX5, and CUBN loci. Recent refinement has pinpointed the major PITX1 determinant to an ∼18-kb deletion adjacent to H2AFY, which suppresses distal gene expression by disrupting a chromatin insulator. To evaluate these variants, we surveyed over 20,000 birds from diverse populations and identified an atypical, mild-to-variable shank-feathering phenotype in five Chinese indigenous breeds and one selected line. This phenotype is characterized by sparse, short feathers that do not produce the classic "booted" appearance and has at a low prevalence (0.94%-4.83%). The trait showed no significant association with sex (P > 0.05), ruling out a sex-linked inheritance pattern. Targeted genotyping of the known major-effect variants, including the refined H2AFY deletion, was performed in 153 atypical birds, 190 clean‑legged controls, and representative heavy/moderate-feathered individuals. Our results reveal a widespread genetic decoupling from established models in atypical cohorts. In Xiangdong chickens, 38.1% of feathered individuals lack any of the known mutations including PITX1/H2AFY, TBX5, and CUBN. The TBX5 variant exhibited significant incomplete penetrance (66.7%) and failed to co-segregation with the trait in Xiangdong chickens (P > 0.05). No additive phenotypic effects were observed in individuals harboring multiple variants (Cochran-Armitage trend test, P = 0.842), and the CUBN variant showed no association across the studied populations (P > 0.05). These findings provide quantitative evidence that atypical ptilopody operates under a genetic architecture independent of the established PITX1-TBX5 variants. Our results suggest greater genetic complexity than previously recognized and underscore the potential for discovering alternative regulatory mechanisms within these indigenous populations. - Source: PubMed
Publication date: 2026/07/08
Huang XunheZhang ZhifengZhong ZhipengDu BingwangWu QiongLi Jingyi - Hepatocellular carcinoma (HCC) is not only a widespread malignant tumor but also the third leading cause of cancer-related deaths. It has been reported that the expression level of H2AFY in HCC cells and tissues is relatively high and it contributes to the progression of HCC. However, its downstream targets remain unclear. - Source: PubMed
Publication date: 2026/05/14
Sun WanliangZhou ShuoLiu ZhongYang SongZhang DengyongWang DongdongZhou HuQi FeiyuZhang MingruiWu BinquanLu ZhengWang Yifei - Feather-related traits in poultry give rise to a vibrant array of visually striking characteristics. Among these, the Yanying chickens, an indigenous breed, displays a variably expressed feathered-leg phenotype. However, the underlying genetic mechanisms remain largely unexplored, making this breed a valuable model for investigation. In present study, we performed Genome-wide association studies (GWAS) based on whole-genome resequencing data (average depth: 10 ×) of 123 chickens, and found a locus on chromosome 13 (chr13: 14.97-15.05 Mb) that is significantly associated with leg feather trait. By gene annotation, we found a coding gene for H2A-histone-family, member-Y (H2AFY), which encodes a histone variant crucial for chromatin architecture and the regulation of developmental genes, was located in this region. We then identified an approximately 18-kb deletion up-stream of H2AFY. Through PCR validation, we confirmed that this structural variation is significantly associated with the feathered-leg trait. The segregation pattern suggests it is a major genetic determinant, though with incomplete penetrance. This study not only deepens our understanding of the genetic mechanisms underlying feather-related traits but also provides a reliable genetic marker, offering a potential target for molecular-assisted selection of external characteristics in chickens. - Source: PubMed
Publication date: 2026/04/25
Li KanglingYi HanXi YangQi JingjingWang QiangYu JieBai YuanBai LiliHuang AnqiLiu Hehe - Patients with juvenile idiopathic arthritis (JIA) frequently exhibit antinuclear antibodies (ANAs), but the specific antigen target recognized by them and the presence of additional autoantibody specificities in patients with JIA remains elusive. - Source: PubMed
Publication date: 2025/09/21
Pitkänen ViolaRemes-Pakarinen TerhiVähäsalo PaulaMöttönen MiljaGrönlund Minna-MaijaArvonen MiikaKnip MikaelVeijola RiittaToppari JormaIlonen JormaLempainen JohannaSchroderus Anna-MariKröger LiisaKinnunen Tuure - Feathered foot is a trait observed in domestic and wild chickens, resulting from the partial or complete transformation of scales on the tarsus, shanks, and toes into feathers. Although previous studies have mapped genomic loci associated with feathered feet, the molecular mechanisms underlying this transformation remain unclear. This study combined whole-genome re-sequencing (WGS) data, transcriptomic analysis, and cellular experiments to investigate the genetic basis of feathered feet in Guangxi native chickens. A genome-wide association study (GWAS) involving 1,735 Guangxi native chickens identified two genomic regions and several candidate genes, including TBX3, TBX5, and H2AFY. RNA sequencing (RNA-seq) and real-time fluorescent quantitative PCR (QRT-PCR) results confirmed that TBX5 is differentially expressed between chickens with scaled and feathered feet, indicating its role as a key candidate gene for feathered feet. The function of TBX5 was subsequently explored at the cellular level using chicken dermal fibroblasts. The results showed that moderate overexpression of TBX5 (20 ng) significantly increased the expression of cell proliferation-related genes. In contrast, excessive overexpression of TBX5 (2.5 μg) and interference with TBX5 inhibited these genes, suggesting a dose-dependent effect of TBX5 on the proliferation of dermal fibroblasts. EdU fluorescence staining, flow cytometry, and migration assays demonstrated that moderate expression of TBX5 can promote the proliferation and migration of dermal fibroblasts, while TBX5 interference produced opposite results. Our findings suggest that TBX5 is a pivotal candidate gene associated with feathered feet in Guangxi native chickens. It is proposed that TBX5 may affect the formation of feathered feet by regulating the proliferation and migration of dermal fibroblasts. - Source: PubMed
Publication date: 2025/02/01
Yang ZhuliangLiu YongcuiLuo JintangXiao CongYuan HongDu WenyaYang Xiurong