AGPAT6 Blocking Peptide
- Known as:
- AGPAT6 Blocking Peptide
- Catalog number:
- 33r-6449
- Product Quantity:
- USD
- Category:
- -
- Supplier:
- Fitzgerald industries international
- Gene target:
- AGPAT6 Blocking Peptide
Related genes to: AGPAT6 Blocking Peptide
- Gene:
- GPAT4 NIH gene
- Name:
- glycerol-3-phosphate acyltransferase 4
- Previous symbol:
- AGPAT6
- Synonyms:
- DKFZp586M1819, LPAAT-zeta, TSARG7
- Chromosome:
- 8p11.21
- Locus Type:
- gene with protein product
- Date approved:
- 2005-07-18
- Date modifiied:
- 2016-10-25
Related products to: AGPAT6 Blocking Peptide
Related articles to: AGPAT6 Blocking Peptide
- Microglial activation is a central mediator of neuroinflammatory and neurodegenerative processes. Growing evidence indicates that dysregulated lipid metabolism and ferroptosis drive microglial dysfunction, yet pharmacological interventions targeting these interconnected pathways remain scarce. Fenofibrate is well-documented for its anti-inflammatory and antioxidant effects; nevertheless, its influence on microglial lipid metabolism and ferroptotic signaling remains unexplored. The present study was designed to examine the effects of fenofibrate on lipid remodeling, oxidative stress, and ferroptosis in human HMC3 microglia activated with lipopolysaccharide (LPS) and interferon-γ (IFN-γ). HMC3 cells were pre-treated with fenofibrate followed by inflammatory activation. Cell viability, cytokine secretion, oxidative stress, lipid droplet (LD) accumulation, and ferroptosis-associated markers were analyzed by ELISA, fluorescence imaging, qRT-PCR, and Western blotting. Fenofibrate decreased the production of TNF-α, IL-1β, and IL-6, restored mitochondrial membrane potential, and suppressed ROS and malondialdehyde (MDA) generation while increasing intracellular glutathione (GSH). The treatment markedly decreased LD accumulation by downregulating the lipid metabolism-related enzymes PLIN2, DGAT1, and GPAT4. Functional assays demonstrated enhanced fatty acid oxidation and restored lipolysis, directly confirming reprogrammed lipid catabolism. Moreover, fenofibrate attenuated ferroptotic stress, evidenced by reduced intracellular Fe²⁺ levels, decreased ACSL4 expression, and significant increase of the ferroptosis-protective enzyme GPX4. These molecular changes were accompanied by improved cell survival and decreased oxidative damage, suggesting that fenofibrate may partially modulate metabolic and and redox balance in activated microglia under in vitro conditions. In conclusion, these findings suggest that fenofibrate may exert protective effects by modulating lipid metabolism and suppressing ferroptosis-related pathways in activated microglia. - Source: PubMed
Publication date: 2026/04/28
Taskesen AhmetHacioglu CeyhanTuncer SibelKilic GuvenTuncer Cengiz - Lipophagy, a selective form of autophagy, is critical for maintaining cellular lipid homeostasis. However, understanding its dynamic regulation and pathophysiological significance in vivo has been hindered by a lack of sensitive and versatile monitoring tools. To address this gap, we generated the tfLiveDrop (mCherry-eGFP-LiveDrop) reporter mouse by integrating a tandem mCherry-eGFP fluorescent probe with the lipid droplet-targeting domain of glycerol-3-phosphate acyltransferase 4 (GPAT4, the rate-limiting enzyme in triacylglycerol synthesis), termed the LiveDrop domain. This model enables real-time, spatiotemporal visualization of lipophagic flux at single-cell resolution in living animals. We initially validated the sensitivity and specificity of the tfLiveDrop reporter in primary renal tubular epithelial cells (TECs). Systemic mapping of lipophagic activity across organs revealed pronounced heterogeneity in basal lipophagic activity under physiological conditions. Furthermore, in a model of Type 2 diabetes, we demonstrated that lipophagic flux is dysregulated in a tissue-specific manner in male mice, underscoring its pivotal role in disease-associated lipid metabolism. Notably, longitudinal tracking during kidney development uncovered a programmed wave of lipophagic activity that is essential for lipid homeostasis during renal maturation. Our findings provide a powerful and versatile platform for in vivo lipophagy research, establishing a foundation for elucidating its functional contributions to metabolic disorders and organ development. - Source: PubMed
Publication date: 2026/04/02
Gong SiqiaoZhang QiaofeiWu HongluanChen XiaocuiLiu LijingChen YongmingFeng ZeSenLi ShangmeiSu HongyongQi JiansongTang JixinYe ZhennanYang ChenLiu Huafeng - Fat deposition plays a crucial role in regulating the production performance and meat quality of broilers. Although the heterogeneity of mammalian adipocytes has been extensively studied, research on the molecular mechanisms underlying differences in lipid droplet accumulation in avian adipocytes remains limited. This study confirmed a significant positive correlation (R > 0.81, < 0.001) between the SSC signal and lipid droplet content via fluorescence staining of lipid droplets, Oil Red O staining, and triglyceride (TG) quantification. Based on this, a label-free sorting strategy using SSC signals was established to sort differentiated chicken preadipocytes, obtaining high lipid droplet (H) and low lipid droplet (L) subpopulations, which were subsequently subjected to transcriptome sequencing and differential gene expression (DEG) analysis, followed by GO and KEGG enrichment analysis. The results indicated no significant differences in the expression of adipogenesis marker genes (, , , , ) between the high lipid droplet (H) and low lipid droplet (L) groups, suggesting that both groups are at similar stages of differentiation. KEGG analysis revealed that both the H vs. NC and L vs. NC comparisons were enriched in common pathways, including the PPAR signaling pathway, ECM-receptor interaction, focal adhesion, cytokine-receptor interaction, and calcium-Apelin signaling pathway, suggesting that both groups of cells had activated the adipogenesis program. GO analysis showed that, in both H vs. NC and L vs. NC comparisons, differentially expressed genes (DEGs) were enriched in biological processes (BPs) related to cell adhesion, nucleosome assembly, chromatin remodeling, and receptor activity, as well as cellular components (CCs) such as the extracellular matrix, cytoskeleton, and nucleosome organization, indicating extensive gene reprogramming and activation of signaling transduction during differentiation. In the H vs. L comparison, enriched pathways included ABC transporters, ECM-receptor interaction, focal adhesion, gap junctions, microtubule-related processes, and neuroactive ligand-receptor interactions, involving lipid transmembrane transport, cytoskeleton stabilization, and signal transduction regulation, suggesting that high lipid droplet cells are more mature in lipid droplet transport, storage, and homeostasis maintenance. GO enrichment results further supported this conclusion, as H vs. L specifically enriched processes related to microtubule-related processes, cell cycle, and redox reactions (BPs), as well as chromosome organization, cytoskeleton, and motor activity (CC/MF), indicating that high lipid droplet cells maintain lipid droplet fusion and metabolic homeostasis via enhanced microtubule transport and antioxidant regulation. Differential gene analysis revealed that the L group upregulated genes associated with fatty acid synthesis and elongation (, , , , ), cholesterol and isoprenoid biosynthesis (, , , , , , ), and fatty acid oxidation (, , , ), reflecting a metabolic characteristic of concurrent lipid synthesis and mobilization; the H group, conversely, upregulated genes associated with lipid droplet formation and storage (, , , , ), lipid transport (, , , , ), and antioxidant defense (, , ), exhibiting a storage and homeostasis-oriented metabolic state. In the NC, L, and H groups, the expression of five genes-, , , , and -showed a gradual increase, suggesting that these genes were associated with preadipocyte differentiation and lipid droplet deposition. In summary, although the high and low lipid droplet subpopulations of chicken preadipocytes exhibit similar differentiation states, they form distinct metabolic orientations. The L group is characterized by active lipid synthesis, fatty acid oxidation, and membrane lipid remodeling, while the H group predominantly features lipid droplet storage, lipid transport, and antioxidant homeostasis. This study highlights the molecular mechanisms underlying the metabolic heterogeneity of avian adipocytes and provides a theoretical basis for poultry fat deposition regulation and genetic improvement. - Source: PubMed
Publication date: 2026/03/12
Wang BoyuLi YantaoWang YakeChen JiayiWang JialiLi XiaopingLi Zhenhui - The quality and flavor of chicken meat are the key factors that influence consumers' purchase decisions. Recent studies have demonstrated that polyphenol can modulate meat quality. In this study, an integrated multi-omics approach was utilized to systematically identify the regulatory effect of dietary supplementation with polyphenols extracts of leaves (PECOL) on chicken flavor. It was found that dietary PECOL supplementation enhanced breast meat flavor and increased fatty acid ethyl ester compounds in the breast muscle. Moreover, PECOL supplementation reshaped the composition and proportions of gut microbiota across multiple taxonomic levels, with a notable enrichment of taxa within the phylum Firmicutes (e.g., ). Furthermore, the addition of PECOL altered the contents of cecal metabolites related to lipid and glucose metabolism, such as PC (14:1(9Z)/21:0), PC (P-16:0/15:1(9Z)), LysoPE (20:4(8Z, 11Z, 14Z, 17Z)/0:0), and glycerol 3-phosphate. Notably, we found that was significantly correlated with the content of these metabolites related to lipid and glucose metabolism. Further analysis revealed that these metabolites might interact with to jointly regulate chicken flavor. These findings further clarify the regulatory role played by PECOL in shaping the flavor of broiler meat. - Source: PubMed
Publication date: 2026/03/04
Ma MantingHe WanxiLin XiajinWang YibingJiang ShouqunYang LiLi GuizhenGu Yao - Metabolic-associated fatty liver disease (MAFLD) is a common metabolic disease with complex pathogenesis and lack of effective treatment. Si-Ni-San (SNS), a traditional Chinese medicine, has emerged as a promising candidate for MAFLD treatment. However, the protective mechanism remains unclear. - Source: PubMed
Publication date: 2026/01/13
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