ALPPL2 Blocking Peptide
- Known as:
- ALPPL2 Blocking Peptide
- Catalog number:
- 33r-6329
- Product Quantity:
- USD
- Category:
- -
- Supplier:
- Fitzgerald industries international
- Gene target:
- ALPPL2 Blocking Peptide
Related genes to: ALPPL2 Blocking Peptide
- Gene:
- ALPG NIH gene
- Name:
- alkaline phosphatase, germ cell
- Previous symbol:
- ALPPL2
- Synonyms:
- GCAP
- Chromosome:
- 2q37.1
- Locus Type:
- gene with protein product
- Date approved:
- 1990-07-06
- Date modifiied:
- 2018-04-16
Related products to: ALPPL2 Blocking Peptide
Related articles to: ALPPL2 Blocking Peptide
- Pluripotent stem cells (PSCs) exist in either a "primed" state or a "naive" state. While several protocols are available to convert primed human PSCs (hPSCs) to the naive-state hPSCs, they often require multiple exogenous factors. Here, we show that the activation of AMP-activated protein kinase (AMPK) or its downstream p38 alone induces naive conversion. Primed hPSCs cultured with activated AMPK-p38 displayed key naive features, including naive marker expression, three-germ-layer differentiation, epigenomic resetting, and increased mitochondrial activity. An AMPK activator-5-aminoimidazole-4-carboxamide-1-β-D-ribofuranoside (AICAR)-synergistically enhances the naive conversion efficiency of reported conversion protocols. Single-cell RNA sequencing (scRNA-seq) with RNA velocity analyses and Totem trajectory mapping identified an intermediate state bridging the primed and naive states. These cells showed three upregulated gene groups: (1) pluripotency genes (e.g., and ), (2) naive state-related genes (e.g., and ), and (3) differentiation-suppressive genes (e.g., and ). These findings establish a simple induction method that illuminates underlying mechanisms and enables broad applications through efficient naive conversion. - Source: PubMed
Publication date: 2026/04/01
Yang ZhennanLiu YajingXu HuaigengYamane JunkoHotta AkitsuFujibuchi WataruYamashita Jun K - Dissecting the genetic components that contribute to the two main subphenotypes of steroid-sensitive nephrotic syndrome (SSNS) using genome-wide association studies (GWAS) strategy is important for understanding the disease. We conducted a multicenter cohort study (360 patients and 1835 controls) combined with a GWAS strategy to identify susceptibility variants associated with the following two subphenotypes of SSNS: steroid-sensitive nephrotic syndrome without relapse (SSNSWR, 181 patients) and steroid-dependent/frequent relapse nephrotic syndrome (SDNS/FRNS, 179 patients). The distribution of two single-nucleotide polymorphisms (SNPs) in and was significant between SSNSWR and healthy controls, and that of two SNPs in and was significant between SDNS/FRNS and healthy controls. Interestingly, rs1047989 in was a candidate locus for SDNS/FRNS but not for SSNSWR. No significant SNPs were observed between SSNSWR and SDNS/FRNS. Meanwhile, chromosome 2:171713702 in was associated with a greater steroid dose (>0.75 mg/kg/d) upon relapse to first remission in patients with SDNS/FRNS (odds ratio = 3.14; 95% confidence interval, 0.97-9.87; = 0.034). rs117014418 in was significantly associated with a decrease in eGFR of greater than 20% compared with the baseline in SDNS/FRNS patients ( = 0.0001). Protein-protein intersection network construction suggested that HLA-DQA1 and HLA-DQB1 function together through GSDMA. Thus, SSNSWR belongs to non-HLA region-dependent nephropathy, and the HLA-DQA/DQB region is likely strongly associated with disease relapse, especially in SDNS/FRNS. The study provides a novel approach for the GWAS strategy of SSNS and contributes to our understanding of the pathological mechanisms of SSNSWR and SDNS/FRNS. - Source: PubMed
Publication date: 2023/09/27
Chan HanNi FenfenZhao BoJiang HuiminDing JuanjuanWang LiWang XiaowenCui JingjingFeng ShipinGao XiaojieYang XueyingChi HuanLee HaoChen XuelanLi XiaoqinJiao JiaWu DaoqiZhang GaofuWang MoCun YupengRuan XiongzhongYang HaipingLi Qiu - This study was conducted to investigate the effects of different proteases alone or in combination on protein digestibility of broilers. In vitro, the properties of four proteases in broilers, including acidic protease (AcP), alkaline protease (AlP), neutral protease (NeP) and keratinase (Ker), on endogenous protease activity and their effects on protein digestibility of common ingredients in broiler diets were investigated using a gut-mimicking model. In vivo, 640 1-day-old male broilers were randomly divided into 8 groups of 10 with 8 replicates of 10 birds per replicate cage. Eight dietary treatments included a corn-soybean meal basal diet (control), and the basal diet with 1.6 U AcP/g, 0.8 U NeP/g, 0.8 U AlP/g, 0.4 U Ker/g, 1.6 U AcP/g + 0.8 U NeP/g, 1.6 U AcP/g + 0.8 U AlP/g, or 1.6 U AcP/g + 0.4 U Ker/g added. The experiment lasted for 31 days. The results showed that the optimum pH values of AcP, NeP, AlP and Ker were 3.0, 9.0, 11.0 and 11.0 in vitro, respectively. Ker recovery proportion was 37.68% at pH 3.3-6.2. AcP alone or in combination with NeP, AlP or Ker increased in vitro crude protein digestibility (IVCPD) and decreased ileal apparent digestibility of crude protein in 31-day-old broilers ( < 0.05). All protease supplementation reduced the ileal apparent digestibility of amino acids compared to the control ( < 0.05). Acidic protease had a positive effect on trypsin and chymotrypsin activities, while AlP and Ker showed a negative effect. In vivo, average daily gain and average daily feed intake were significantly ( < 0.05) increased in broiler diets supplemented with AcP compared to the control group. When adding exogenous proteases to broiler diets, their sensitivity to digestive pH and their negative effects on endogenous protease activity, dosage and combination effects should be taken into account. In addition, the properties and dosage of proteases and the protein level in the feed should be considered. - Source: PubMed
Publication date: 2023/05/25
Zheng MengliBai YanSun YingxiaAn JingChen QinghuaZhang Tieying - Recently, the successful delivery of organs for transplantation using drones was reported. We investigated the influence of transportation by drones on the quality of liver grafts using a rat model. - Source: PubMed
Publication date: 2023/09/24
Enjoji TakahiroSoyama AkihikoFukumoto MasayukiPeilin LiMatsuguma KunihitoImamura HajimeMaruya YasuhiroHara TakanobuMatsushima HajimeKugiyama TotaAdachi TomohikoHidaka MasaakiHamamoto ShoTakashima ShiroMaeda TakahiroKanetaka KengoEguchi Susumu - Neurosteroids (NSs) are endogenous steroid hormones, which are synthesised and metabolised within the central nervous system (CNS). NSs aid myelination and glial differentiation and modulate cognitive functions. Herein, we aim to investigate the relationship between NS levels, 5-alpha-dihydroxyprogesterone (5-α-DHP) and allopregnanolone (ALPG), and their relationship with cognitive changes in relapsing remitting MS patients.A total of 43 cases with well controlled, relapsing remitting MS composed the study group. The control group included 21 age and gender matched healthy controls (HC). MS patients were assessed by calculating Expanded Disability Status Scale (EDSS) scores, and the Brief Repeatable Battery of Neuropsychological Tests (BRBNT) was performed in both MS group and HC. Levels of 5-α-DHP and ALPG levels were also evaluated for each participant.The median level of 5-α-DHP was 48 [IQR: 39.2-144.2] pg/mcgL in the MS group and 68.4 [IQR: 57.1-365.9] pg/mcgL in HC ( = 0.02). The median ALPG level was found to be 56.5 [IQR: 37.7-75.4] pg/mcgL in the MS group and 43.9 [IQR: 29.4-70.2] pg/mcgL in HC ( = 0.1). In both groups 5-α-DHP levels were positively correlated with Symbol Digit Modalities Test (SDMT) scores (HC: = 0.01, = 0.3 and MS: = 0.03, = 0.3). In the MS group, higher EDSS scores were associated with lower scores on Spatial Recall Test (SPART)-Delayed ( = 0.009, = -0.4) and SDMT ( = 0.01, = -0.4). The disease duration was negatively correlated with the scores on SPART-Immediate, SPART-Delayed and SDMT ( = 0.02, = -0.4; = 0.005, = -0.4 and = 0.05, = -0.3).5-α-DHP may be lower even in well-controlled cases. 5-α-DHP may contribute to better perceptual processing and attention in cases with MS. - Source: PubMed
Publication date: 2024/01/01
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