Ask about this productRelated genes to: TIMM44 Blocking Peptide
- Gene:
- TIMM44 NIH gene
- Name:
- translocase of inner mitochondrial membrane 44
- Previous symbol:
- -
- Synonyms:
- TIM44
- Chromosome:
- 19p13.2
- Locus Type:
- gene with protein product
- Date approved:
- 2002-01-15
- Date modifiied:
- 2016-03-07
Related products to: TIMM44 Blocking Peptide
Related articles to: TIMM44 Blocking Peptide
- Hyperglycemic conditions up - regulate TIMM44, an inner mitochondrial membrane protein. Its expression is related to many cancers, yet its role in gastric cancer (GC) remains unproven. To study this, we used western blot and qRT - PCR to measure protein and mRNA levels. Flow cytometry analyzed cell cycle progression. CCK - 8, colony formation, wound healing, Transwell, and EdU assays evaluated cell viability, migratory and invasion abilities. TUNEL - based method detected apoptosis, and immunohistochemical assay compared TIMM44 expression in GC and adjacent non - cancerous tissues. The results showed TIMM44 was highly expressed in GC tissues. Over - expressing TIMM44 promoted AGS and HGC27 cells' viability, proliferation, invasion, and migration, while its depletion inhibited these. Forced TIMM44 expression increased Gαi1 and Akt phosphorylation. In conclusion, TIMM44 is crucial in the Gαi1 - PI3K - AKT - mTOR pathway, enhancing GC cells' malignancy and potentially threatening patient survival. - Source: PubMed
Publication date: 2026/05/05
Chen TingXu RongShi XiaojingYang DongliangDu YiSong YuLv YanChen Yanan - Functional perturbations of genes do not always cause expression changes, but can manifest through network rewiring or context-specific shifts in regulatory activity. However, inferring functional shifts in genes and linking them to the specific cell populations remains challenging, as most current scRNA-seq data analysis focuses either on differential gene expression or on cell abundance/state changes, but rarely associate gene perturbations with particular cell populations. Here we present scDNS, a framework that quantifies gene-specific functional perturbations by measuring information-theoretic divergence between condition-specific gene interaction network configurations. In simulated stress tests and multiple experimental datasets, scDNS prioritizes key regulators and perturbed cell populations, even when expression changes are minimal but network rewiring is pronounced. Applications to immunodeficiency mutations, stimulus responses, and viral infection reveal hidden regulatory programs and heterogeneous responder cell states. In pancreatic cancer, scDNS nominates TIMM44 as a mitochondrial sensitizer enhancing gemcitabine efficacy. Together, scDNS provides a powerful tool for inferring dynamic gene perturbations in single cells. - Source: PubMed
Publication date: 2026/04/11
Huang ChaoLi YuhanFa BotaoZhu JinglinLiu ZhenniMa YixinZhang ZhitaoXu YungangXu QiuranXiao Zhengtao - Gestational diabetes mellitus (GDM) is a pregnancy-related disorder characterized by inflammatory dysregulation that disrupts maternal-fetal immune homeostasis, yet the contribution of mitochondrial dysfunction to this pro-inflammatory state remains incompletely understood. - Source: PubMed
Publication date: 2026/02/26
Zhao RuiChai TingtingGao QinJiang Aimin - Colorectal cancer (CRC) ranks as the third most common global cancer. This study aims to explore the expression, function, and mechanism of DEAD-box helicase 49 (DDX49) in CRC. - Source: PubMed
Publication date: 2025/12/08
Huang BaoyuYin ZhipengGao GangLi LonghaiYang Miao - MFN1 (mitofusin 1) and MFN2 are key players in mitochondrial fusion, endoplasmic reticulum (ER)-mitochondria juxtaposition, and macroautophagy/autophagy. However, the mechanisms by which these proteins participate in these processes are poorly understood. Here, we studied the interactomes of these two proteins by using CRISPR-Cas9 technology to insert an HA-tag at the C terminus of MFN1 and MFN2, and thus generating HeLa cell lines that endogenously expressed MFN1-HA or MFN2-HA. HA-affinity isolation followed by mass spectrometry identified potential interactors of MFN1 and MFN2. A substantial proportion of interactors were common for MFN1 and MFN2 and were regulated by nutrient deprivation. We validated novel ER and endosomal partners of MFN1 and/or MFN2 with a potential role in interorganelle communication. We characterized RAB5C (RAB5C, member RAS oncogene family) as an endosomal modulator of mitochondrial homeostasis, and SLC27A2 (solute carrier family 27 (fatty acid transporter), member 2) as a novel partner of MFN2 relevant in autophagy. We conclude that MFN proteins participate in nutrient-modulated pathways involved in organelle communication and autophagy.: ACTB: actin, beta; ATG2: autophagy related 2; ATG5: autophagy related 5; ATG12: autophagy related 12; ATG14: autophagy related 14; ATG16L1: autophagy related 16 like 1; Baf A1: bafilomycin A; BECN1: beclin 1, autophagy related; BFDR: Bayesian false discovery rate; Cas9: CRISPR-associated endonuclease Cas9; CRISPR: clustered regularly interspaced short palindromic repeats; DNM1L/DRP1: dynamin 1-like; ER: endoplasmic reticulum; Faa1: fatty acid activation 1; FC: fold change; FDR: false discovery rate; FIS1: fission, mitochondrial 1; GABARAP: gamma-aminobutyric acid receptor associated protein; GABARAPL2: GABA type A receptor associated protein like 2; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; HA: hemagglutinin; KO: knockout; LIR: LC3-interacting region; MAP1LC3B/LC3B: microtubule-associated protein 1 light chain 3 beta; MARCHF5: membrane associated ring-CH-type finger 5; MDVs: mitochondria-derived vesicles; MFN1: mitofusin 1; MFN2: mitofusin 2; NDFIP2: Nedd4 family interacting protein 2; OMM: outer mitochondrial membrane; OPA1: OPA1, mitochondrial dynamin like GTPase; OXPHOS: oxidative phosphorylation; PE: phosphatidylethanolamine; PINK1: PTEN induced putative kinase 1; PS: phosphatidylserine; RAB5C: RAB5C, member RAS oncogene family; S100A8: S100 calcium binding protein A8 (calgranulin A); S100A9: S100 calcium binding protein A9 (calgranulin B); SLC27A2: solute carrier family 27 (fatty acid transporter), member 2; TIMM44: translocase of inner mitochondrial membrane 44; TOMM20: translocase of outer mitochondrial membrane 20; ULK1: unc-51 like kinase 1; VCL: vinculin; VDAC1: voltage-dependent anion channel 1; WT: wild type. - Source: PubMed
Publication date: 2024/12/24
Gordaliza-Alaguero IsabelSànchez-Fernàndez-de-Landa PaulaRadivojevikj DraganaVillarreal LauraArauz-Garofalo GianlucaGay MarinaMartinez-Vicente MartaSeco JorgeMartín-Malpartida PauVilaseca MartaMacías María JPalacin ManuelIvanova SaškaZorzano Antonio