Ask about this productRelated genes to: TFAM Blocking Peptide
- Gene:
- TFAM NIH gene
- Name:
- transcription factor A, mitochondrial
- Previous symbol:
- TCF6, TCF6L2
- Synonyms:
- -
- Chromosome:
- 10q21.1
- Locus Type:
- gene with protein product
- Date approved:
- 1991-09-13
- Date modifiied:
- 2016-10-05
Related products to: TFAM Blocking Peptide
Related articles to: TFAM Blocking Peptide
- The retina is considered one of the most metabolically active tissues in the mammalian central nervous system. Prior studies have defined the extent of metabolic diversity among all seven adult retinal cell types. However, the specific metabolic changes in retinal progenitor cells (RPCs) during development remain unknown. In this study, we characterized the histochemical patterns of oxidative phosphorylation (OXPHOS) and glycolysis indicators in the differentiating and maturing mouse retina. Also, by performing RPC-specific conditional knockout (CKO) of the mitochondrial transcription factor A (Tfam), we generated retinas in which RPC OXPHOS activity was lost throughout retinogenesis. We found that early-stage (embryonic) Tfam CKO RPCs were completely OXPHOS-deficient yet underwent normal proliferation, cell cycle exit, and differentiation into retinal neurons. In contrast, late-stage (postnatal) RPCs remained in a proliferative state well past the normal developmental period, leading to a later reduction in the generation of bipolar cells and Müller glia. Ultimately, as the CKO retina fully matures, it becomes severely hypoplastic due to cell death that we attribute to the previous loss of bipolar cells and Müller glia. In total, our data establishes that, over time, RPCs exhibit heterogeneity in their metabolic requirements, with the second wave of RPCs being more reliant on OXPHOS activity to undergo proper neurogenesis. - Source: PubMed
Publication date: 2026/06/17
Rueda Elda MWasel EllaSchubert Carlina MBarrasso Anthony PTong XuefeiPoché Ross A - To improve ovarian tissue transplantation effectiveness by limiting oxidative stress after transplantation, thus increasing follicle survival. - Source: PubMed
Publication date: 2026/06/17
Adrados Cristina SubiranOlesen Hanna ØrnesOlesen Sophie VernimmenZeuthen Mette ChristaPors Susanne ElisabethPedersen Anette TønnesKristensen Stine Gry - Oncogenic condensates act as biophysical sanctuaries that stabilize malignant survival programs. However, a universal regulator capable of orchestrating the integrated biophysical axes governing cellular phase behavior has remained elusive. Here, we introduce a sovereign singularity framework, presenting a deductive biophysical model that positions the indoleamine melatonin as a master regulator of biological phase separation. A systematic synthesis and integrative bioinformatics analysis were performed to identify the intersection between melatonin-responsive genes and the phase-separation proteome. We identified a core 26-gene regulatory signature-including AR, BCL2, CGAS, CTNNB1, EP300, EZH2, EGFR, IKBKG (NEMO), KEAP1, KDM1A (LSD1), LEF1, MYC, NANOG, PRNP (PRP), SMAD3, SOX9, SQSTM1, TFEB, TFAM, TP53, TWIST1, USP10, WWTR1 (TAZ), VIM, YAP1, and YTHDF3-at the intersection of melatonin signaling and condensate architecture. We propose that melatonin utilizes a tri-lever framework of redox tuning (Lever I), multivalent plasticization (Lever II), and dielectric recalibration (Lever III) to render oncogenic programs biophysically untenable. This model provides a mechanical basis for high-resolution regulatory outcomes that modulate the organizational logic of nuclear decision-making (Axis I), state-transition (Axis II), and stress-adaptation (Axis III) condensates. Our results define a strategic platform for disrupting condensate-driven malignancy through the systemic modulation of the cellular biophysical landscape. - Source: PubMed
Loh DorisChuffa Luiz Gustavo de AlmeidaSeiva Fábio Rodrigues FerreiraReiter Russel J - While mesenchymal stromal cell (MSC)-derived extracellular vesicles (MSC-EVs) offer a safer, cell-free alternative to stem cell transplantation, their specific role in rescuing recipient cell mitochondrial networks requires precise definition. This review clarifies that scientific landscape by systematically partitioning MSC-EV-mediated mitochondrial delivery into three rigorous, evidence-based categories: (i) the horizontal transfer of intact, bioenergetically active mitochondria, (ii) the lateral delivery of sub-organellar components such as mitochondrial DNA (mtDNA) and transcriptional proteins (e.g., TFAM), and (iii) indirect protective signaling that rejuvenates endogenous networks. Effectively integrated cargo within MSC-EV has been reported to restore mitochondrial membrane potential, contributing to the stabilization of electron transport chain complexes (I-IV), the reactive oxygen species (ROS) balance, and the tricarboxylic acid (TCA) cycle and NAD + /NADH balance to reverse bioenergetic collapse. Across diverse myocardial, pulmonary, hepatic, renal, and neurological injury models, this EV-associated delivery is associated with dampening of hyper-inflammation, enhances macrophage phagocytosis, and supports tissue barrier regeneration. Nevertheless, critical translational barriers remain, including significant EV heterogeneity, a lack of standardized high-purity isolation protocols in line with MISEV (Minimal Information for Studies of Extracellular Vesicles) guidelines, and unverified oncologic risks such as supporting tumor progression or chemoresistance through unintended metabolic rescue. In conclusion, large-scale clinical adoption requires prioritized, well-designed human trials with rigorous cargo characterization to firmly establish long-term safety, durability, and oncologic security. - Source: PubMed
Publication date: 2026/06/17
Ma TingkunHe YuanYang JincuiLuo PengShen QiyiYang JunjieXu DingLi XiaoDeng ChunchuLi KeluChen BiaoShi Yulong - The avascular nature of cartilage hinders drug delivery for osteoarthritis (OA) therapy. We engineered a biomimetic piezoelectric hydrogel (CMB Gel) by embedding chondrocyte membrane-camouflaged, CAP peptide-grafted barium titanate nanoparticles into a dynamic borate ester-crosslinked network, enabling active cartilage targeting and on-demand ultrasound activation. Under ultrasound, the piezoelectric component generates local electrical signals, triggering Ca influx via voltage-gated calcium channels and AMPK activation. Activated AMPK inhibits the mTOR pathway, inducing epigenetic reprogramming via H3K27 acetylation at the GATD3A promoter. Upregulated GATD3A stabilizes TFAM and enhances PINK1/Parkin-mediated mitophagy, clearing damaged mitochondria and reducing oxidative stress in chondrocytes. In a mouse destabilization of medial meniscus model, ultrasound-activated CMB Gel attenuated cartilage degradation, osteophyte formation, and synovitis. Its therapeutic efficacy was validated in human OA cartilage explants. This work presents a multifunctional targeted delivery platform that converts mechanical energy into epigenetic signals to restore cellular homeostasis, offering a promising strategy for OA and other mechanosensitive degenerative diseases. - Source: PubMed
Publication date: 2026/06/16
Zheng HuiYan PengfeiLiu PengYan ChangZhao MengqiWang ZuyongMa ChaoZhang RongkaiCui XiTeoh Swee Hin