KAP8.1 Blocking Peptide
- Known as:
- KAP8.1 Blocking Peptide
- Catalog number:
- 33r-4817
- Product Quantity:
- USD
- Category:
- -
- Supplier:
- Fitzgerald industries international
- Gene target:
- KAP8.1 Blocking Peptide
Related products to: KAP8.1 Blocking Peptide
Related articles to: KAP8.1 Blocking Peptide
- Mohair, an important source of fiber, is only obtained from Angora goats. The important characteristics that determine the economic value of mohair are fiber diameter and quantity. In countries where mohair is produced, efforts are made to improve these characteristics. It is stated that hair follicle characteristics and/or genetic regulation mechanisms that form animal fibers directly affect fiber production and quality. In this study, it was aimed to determine the genes affecting mohair development in two varieties of Angora goat and the molecular mechanisms affecting these genes. The biopsy samples were collected during three distinct phases of the hair growth cycle: anagen (active growth, September), catagen (transition, January), and telogen (resting, March). The specific timing and location of the biopsies suggest a potential influence of seasonality or a controlled experimental design. RNA was isolated from these biopsy samples, and differentially expressed genes (DEGs) and the pathways affected by these genes were evaluated using the RNA sequencing method. It has been observed that the expression of , , and genes was quite high in the group in which catagen and telogen phases were compared in Eskisehir variety females. Interestingly, the gene was found to be expressed in four different protein isoforms. Interestingly, the analysis revealed a cluster of keratin-associated protein genes (, , ) solely differentially expressed (DE) in the male Eskisehir versus Lalahan comparison. This suggests potential sex-specific regulatory mechanisms involving keratinocyte differentiation during the catagen phase, which might be unique to the Eskisehir variety. In the Eskisehir variety, unlike the Lalahan variety, DEGs identified in the anagen-catagen comparison in males were significantly enriched in the Reactome mediated keratinization pathway, and these genes were down regulated. The results showed that and genes are highly functional and have different expression patterns between males and females, in addition to being different between the Eskisehir and Lalahan varieties. This study provides valuable insights into the genetic regulation of mohair development, potentially paving the way for targeted breeding strategies to improve mohair quality and production. - Source: PubMed
Publication date: 2026/01/31
Selçuk Senem EsinOzmen OzgeÇolak Reyhan - In this study, keratin-associated proteins gene () from five different sheep breeds and breed-crosses ( = 310) was genotyped using a Polymerase Chain Reaction-Single Strand confirmation Polymorphism (PCR-SSCP). Six unique genotypes were observed: and , with being the most common in the different breeds and crosses. Twelve wool characteristics: yield, mean staple length (MSL), bulk, mean fiber diameter (MFD), fiber diameter standard deviation (FDSD), coefficient of variation of fiber diameter (CVFD), medullation, standard deviation of medullation (MeSD), coefficient of variation of medullation (CVMed), opacity, standard deviation of opacity (OpSD), and coefficient of variation of opacity (CVOp) were measured on wool derived from the sheep. Variation in was found to have strong association with MSL, OpSD and CVOp ( ≤ 0.027). The MSL of sheep of genotype was greater ( = 0.027) than for sheep of genotype . The OpSD of sheep of genotype was less ( = 0.017) than sheep with the genotype, and the CVOp of sheep with genotype was less ( = 0.018) than sheep with genotype . Further studies are required to confirm the role of variation in in improving quality wool production. - Source: PubMed
Publication date: 2021/10/19
Ullah FarmanJamal Syed MZhou HuitongHickford Jon G H - Lung adenocarcinoma (LUAD) is one of the most common cancers and lethal diseases in the world. Recognition of the undetermined lung nodules at an early stage is useful for a favorable prognosis. However, there is no good method to identify the undetermined lung nodules and predict their clinical outcome. DNA methylation alteration is frequently observed in LUAD and may play important roles in carcinogenesis, diagnosis, and prediction. This study took advantage of publicly available methylation profiling resources and a machine learning method to investigate methylation differences between LUAD and adjacent non-malignant tissue. The prediction panel was first constructed using 338 tissue samples from LUAD patients including 149 non-malignant ones. This model was then validated with data from The Cancer Genome Atlas database and clinic samples. As a result, the methylation status of four CpG loci in homeobox A9 (), keratin-associated protein 8-1 (), cyclin D1 (), and tubby-like protein 2 () were highlighted as informative markers. A random forest classification model with an accuracy of 94.57% and kappa of 88.96% was obtained. To evaluate this panel for LUAD, the methylation levels of four CpG loci in , and of tumor samples and matched adjacent lung samples from 25 patients with LUAD were tested. In these LUAD patients, the methylation of was significantly upregulated, whereas the methylation of , and were downregulated obviously in tumor samples compared with adjacent tissues. Our study demonstrates that the methylation of , and has great potential for the early recognition of LUAD in the undetermined lung nodules. The findings also exhibit that the application of improved mathematic algorithms can yield accurate and particularly robust and widely applicable marker panels. This approach could greatly facilitate the discovery process of biomarkers in various fields. - Source: PubMed
Publication date: 2019/12/03
Shen NanDu JunZhou HuiChen NanPan YiHoheisel Jörg DJiang ZonghuiXiao LingTao YueMo Xi - Keratin-associated protein 8.1 (KAP8.1) is a hair protein whose structure, biochemical roles, and protein distribution patterns have not been well characterized. In this study, we generated a monoclonal antibody against human KAP8.1 to analyze the protein's roles and distribution in the human hair shaft. Using this antibody, we revealed that KAP8.1 was predominantly expressed in discrete regions of the keratinizing zone of the hair shaft cortex. The protein expression patterns paralleled the distribution of KAP8.1 mRNA and suggested that KAP8.1 plays a role associated with cells to control hair curvature. Cross-reactivity among species and epitope analysis indicated that the monoclonal antibody recognized a linear epitope shared among human, mouse, and sheep KAP8.1. The antibody failed to interact with sheep KAP8.1 in native conformation, suggesting that structural features of KAP8.1 vary among species. - Source: PubMed
Publication date: 2018/08/18
Akiba HirokiIkeuchi EminaGanbat JavkhlanFujikawa HirokiArai-Kusano OsamuIwanari HirokoNakakido MakotoHamakubo TakaoShimomura YutakaTsumoto Kouhei - The keratin-associated proteins (KAPs) are fundamental components of hair and wool fibres, and are believed to in part be responsible for some of the properties of these fibres. KAPs can be divided into three groups: the high sulphur (HS) KAPs, the ultra-high sulphur (UHS) KAPs and the high glycine-tyrosine (HGT) KAPs. KAP8 is a HGT-KAP family and was believed to be coded for by a single gene in both humans and sheep. However, the recent identification of a KAP8-2 gene in goats led us to investigate whether a KAP8-2 gene exists in sheep. A BLAST search of the Ovine Genome Assembly v2.0 using the coding sequence of caprine KRTAP8-2 identified a homologous region on sheep chromosome 1 (OAR1:123005473_123005664; E = e(-101)). This region was clustered with a number of previously identified KAP genes including (in order from the centromere) KRTAP11-1, KRTAP7-1, KRTAP8-1, KRTAP6-2, KRTAP6-1, KRTAP13-3 and KRTAP24-1. PCR-SSCP analysis of the notional gene revealed two dissimilar PCR-SSCP banding patterns, representing two DNA sequences. A single nucleotide difference 21 bp upstream of the TATA box was identified. The two sequences did not have great homology with known ovine KRTAP sequences, but high sequence identity was found with KRTAP8-2 from goats and reindeer. These results suggest that sheep possess a KAP8-2 gene and that this gene is polymorphic. The notional KAP8-2 protein is comprised of 63 amino acid residues and is rich in glycine and tyrosine, but has a low cysteine content. In contrast to other HGT-KAPs, ovine KAP8-2 contains more acidic amino acid residues, and this would likely result in a lower isoelectric point (pI) of 6.3. - Source: PubMed
Publication date: 2014/09/15
Gong HuaZhou HuitongDyer Jolon MHickford Jon Gh