Ask about this productRelated genes to: RASSF1 Blocking Peptide
- Gene:
- RASSF1 NIH gene
- Name:
- Ras association domain family member 1
- Previous symbol:
- -
- Synonyms:
- NORE2A, REH3P21, RDA32, 123F2
- Chromosome:
- 3p21.31
- Locus Type:
- gene with protein product
- Date approved:
- 2000-05-30
- Date modifiied:
- 2016-10-05
- Gene:
- RASSF1-AS1 NIH gene
- Name:
- RASSF1 antisense RNA 1
- Previous symbol:
- -
- Synonyms:
- ANRASSF1
- Chromosome:
- 3p21.3
- Locus Type:
- RNA, long non-coding
- Date approved:
- 2013-09-01
- Date modifiied:
- 2018-09-07
Related products to: RASSF1 Blocking Peptide
Related articles to: RASSF1 Blocking Peptide
- The locus encoding Ras association domain family member 1 (RASSF1) encodes multiple transcripts with opposing roles in cancer, such as RASSF1A (tumor suppressor), RASSF1C (oncogene), and the lncRNA RASSF1-AS1 (function undefined). Although DNA methylation-mediated repression of RASSF1A expression has been extensively studied in different cancer types, the epigenetic regulation of RASSF1C and RASSF1-AS1 is unclear. We profiled gene expression and promoter DNA methylation of RASSF1A, RASSF1C, and RASSF1-AS1 across 11 tumor cell lines, quantified RASSF1A methylation in lung cancer tissues and plasma by quantitative methylation-specific PCR (qMSP), and integrated single-CpG methylation (pyrosequencing) with in silico transcription factor binding site (TFBS) prediction. - Source: PubMed
Bermudez Litzy GisellaForigua Camila BernalAyala-Roldán Carmen MaríaRomero Jesús ManuelAriza Rafael RMorales-Ruiz TeresaRoldán-Arjona TeresaCañas AlejandraRojas Adriana - Alternative protein-coding transcripts of the gene have been associated with dual functions in human cancer: while isoform has oncogenic properties, is a tumour suppressor frequently silenced by hypermethylation. Recently, the antisense long non-coding RNA RASSF1 ( was implicated in a -specific mechanism for the epigenetic repression mediated by PRC2 (Polycomb Repressive Complex 2). Here, we evaluated the methylation patterns of the promoter regions of and and the expression levels of these transcripts in breast cancer and breast cancer cell lines. As expected, remained unmethylated and was hypermethylated at high frequencies in 75 primary breast cancers, and also in a panel of three mammary epithelial cells (MEC) and 10 breast cancer cell lines (BCC). Although was expressed in all cell lines, only two of them expressed the transcript expression levels were increased in six BCCs. induced demethylation with 5-Aza-2'-deoxicytydine (5-Aza-dC) resulted in up-regulation of and an inverse correlation with relative abundance in BCCs. However, increased levels of both transcripts were observed in two MECs (184A1 and MCF10A) after treatment with 5-Aza-dC. Overall, these findings indicate that is differentially expressed in MECs and BCCs. The lncRNA provides new perspectives as a therapeutic target for -specific regulation of . - Source: PubMed
Publication date: 2019/05/27
Calanca NaiadePaschoal Ana PaulaMunhoz Érika PrandoGalindo Layla TestaBarbosa Barbara MitsuyasuCaldeira José Roberto FígaroOliveira Rogério AntonioCavalli Luciane ReginaRogatto Silvia ReginaRainho Cláudia Aparecida - Cardiac fibrosis is associated with various cardiovascular diseases and can eventually lead to heart failure. Dysregulation of long non-coding RNAs (lncRNAs) are recognized as one of the key mechanisms of cardiac diseases. However, the roles and underlying mechanisms of lncRNAs in cardiac fibrosis have not been explicitly defined. Here, we investigated the role of an antisense (AS) lncRNA from the Ras association domain-containing protein 1 isoform A (RASSF1A) gene locus, named RASSF1-AS1, in the development of cardiac fibrosis. Cardiac fibrosis mouse model was established by isoproterenol injection. We found that RASSF1A protein was downregulated, whereas RASSF1-AS1 was markedly upregulated during cardiac fibrosis. Overexpression and knockdown of mouse primary cardiac fibroblasts showed that RASSF1-AS1 negatively regulated RASSF1A expression at the post-transcriptional level. According to the landscape analysis and sense-AS binding evaluation, RASSF1-AS1 partially overlaps with RASSF1A messenger RNA (mRNA) at the exon2 region. RNA pull-down and luciferase activity assays confirmed that RASSF1-AS1 directly bound to RASSF1A mRNA and suppressed its translation. Furthermore, wild-type RASSF1-AS1 had a promoting effect on nuclear factor-κB activation and cardiac fibrosis, but mutated RASSF1-AS1, in which the binding region was deleted, had no effect. In conclusion, RASSF1-AS1 inhibits the translation of RASSF1A to exacerbate cardiac fibrosis in mice, indicating a potential application of RASSF1-AS1 as a therapy target for cardiac fibrosis. - Source: PubMed
Publication date: 2019/08/09
Guo MinLiu TangyuZhang ShujieYang Longbiao