DNA2L Blocking Peptide
- Known as:
- DNA2L Blocking Peptide
- Catalog number:
- 33r-4506
- Product Quantity:
- USD
- Category:
- -
- Supplier:
- Fitzgerald industries international
- Gene target:
- DNA2L Blocking Peptide
Related genes to: DNA2L Blocking Peptide
- Gene:
- DNA2 NIH gene
- Name:
- DNA replication helicase/nuclease 2
- Previous symbol:
- DNA2L
- Synonyms:
- KIAA0083
- Chromosome:
- 10q21.3
- Locus Type:
- gene with protein product
- Date approved:
- 1996-06-10
- Date modifiied:
- 2016-10-05
Related products to: DNA2L Blocking Peptide
Related articles to: DNA2L Blocking Peptide
- Precise regulation of enzyme recruitment during Okazaki fragment maturation (OFM) is essential for faithful and efficient lagging-strand DNA synthesis. Emerging evidence suggests that PARP1 contributes to OFM yet its specific functions remain unclear. Here, we define context-dependent functions of PARP1 during OFM. Under physiological conditions, PARP1 co-localizes with PCNA in early S phase and restrains Pol δ-PCNA- mediated strand-displacement DNA synthesis, thereby preventing the formation of long 5' flaps, which is refractory to FEN1 cleavage. On the other hand, in LIG1-deficient cells, in which DNA nicks and unexpectedly long 5' flaps accumulate, PARP1 promotes the recruitment of LIG3 to catalyze OF ligation and DNA2 to facilitate long 5' flap processing. Collectively, our findings uncover previously unrecognized roles of PARP1 in regulating 5' flap dynamics to ensure efficient OFM and cell viability. - Source: PubMed
Publication date: 2026/04/30
Shi GuojunWang YixingYan YaoLi KejiaoMa LingzhiLei YiWang YingyingManriquez NancyZhou MianZha ShanZheng LiShen Binghui - Mercury ions (Hg) are highly toxic and pose severe risks to human health and ecosystems, necessitating sensitive detection methods for environmental monitoring. Here, we report a paper-based graphene sensor functionalized with single-stranded DNA (ssDNA) probes for Hg detection based on T-Hg-T coordination chemistry. To elucidate the effect of probe structure on sensing performance, we designed DNA constructs with varying numbers of guanine (G) bases (3-6, designated DNA2-DNA5) in the bridging fragment and systematically evaluated their influence on hairpin stability, Hg binding affinity, and sensor response. The DNA3-based sensor (four G bases) exhibited optimal electronic stability and sensitivity, achieving a detection limit of 0.673 pM with effective real-time monitoring capability in aqueous media. These findings highlight the critical role of DNA sequence design in T-Hg-T-based biosensors and provide a promising strategy for sensitive and selective Hg detection in environmental samples. - Source: PubMed
Publication date: 2026/04/10
Wu ZihaoLi JingyanShi HaixiaXie BingGao Li - A straightforward and practicable method to identify and detect low concentrations of small molecule microcystin-LR (MC-LR) is essential to clinical diagnostics, drug development, and environmental and food analysis. Here, we developed a novel electrochemiluminescence (ECL) aptasensor for sensitive detection of MC-LR based on DNA-mediated synergistic proximity ligation assay (PLA). The capture DNA was immobilized on the modified glassy carbon electrode with Ru(bpy) (as the ECL signal), Nafion and gold nanoparticles (AuNPs) on its surface a Au-S bond to obtain the ECL aptasensor. DNA1 was strategically designed with dual ferrocene (Fc) groups as quenchers at both its 5' and 3' ends, enabling simultaneous hybridization with capture DNA, MC-LR aptamer, and DNA2 which also contained a Fc group. In the absence of MC-LR, a proximate cooperative complex was formed among capture DNA, MC-LR aptamer, DNA1, and DNA2, drawing the Fc groups near the electrode surface and leading to a lower ECL signal. Conversely, in the presence of MC-LR, the target molecule MC-LR competed with capture DNA, DNA1, and DNA2 for the aptamer a competitive reaction mode in a homogeneous solution, impeding the formation of a proximate cooperative complex and keeping the Fc groups far away from the electrode surface with a higher ECL intensity. The recovered ECL intensity rose in accordance with the MC-LR concentration, enabling the detection of MC-LR as low as 1.4 pg mL with a dynamic range of 0.005 ng mL to 5.0 ng mL. Importantly, this method's synergistic proximity ligation effect was validated by comparing the ECL signals of the "4-DNA cooperative complex" "3-DNA cooperative complex" under identical conditions. The stronger ECL intensity observed in the "4-DNA cooperative complex" confirmed the synergistic proximity ligation effect, highlighting its critical role in facilitating the DNA hybridization. The developed PLA-based ECL aptasensor demonstrated high sensitivity and selectivity and quick response and it was successfully applied to the detection of MC-LR in tap water. The novelty of this work lies in the strategic design of a dual-quencher DNA probe and the implementation of a competitive, synergistic PLA, which together enable ultrasensitive detection of small molecules like MC-LR, a challenge for traditional PLA methods. - Source: PubMed
Publication date: 2026/04/23
Gao HongfangXiao RongMa TaotaoYang XiaolinMa Shangxian - DNA replication helicase/nuclease 2 (DNA2) is an evolutionarily conserved nuclease-helicase with known role in maintaining nuclear genome stability. However, its potential involvement in metabolic regulation and disease remains unclear. This study investigates the role of DNA2 in pancreatic β cell physiology and diabetes pathogenesis. - Source: PubMed
Publication date: 2026/03/04
Xu HaixiaTang DongmeiYang NaMa MeilinTian YanFu Xianghui - The late-onset axonal Charcot-Marie-Tooth type 2T (CMT2T) is a form of sensorimotor peripheral polyneuropathy caused by mutations in the membrane metalloendopeptidase (MME) gene and inherited either as an autosomal recessive (AR) or as an autosomal dominant (AD) manner with incomplete penetrance. Here, we describe the clinical presentations and genetic profiles of 11 unrelated Iranian families (15 cases) diagnosed with CMT2T. Whole exome sequencing (WES) was utilized to detect genetic variants, and Sanger sequencing was subsequently performed to validate the identified variants. Among our patients, 13 exhibited AR-CMT2T, whereas two presented AD-CMT2T. Altogether, six variants in MME were detected, four of which were novel. The known c.499T>A, the most prevalent, and c.1861T>C variants in total occurred in 7/11 families. Interestingly, in one patient, an additional likely-pathogenic variant was identified alongside the MME variant within the DNA2 gene. His muscle biopsy revealed mixed neurogenic and rimmed vacuole myopathic changes. Our findings highlight the clinical and genetic heterogeneity among CMT2T patients. The presence of both c.499T>A and c.1861T>C variants in multiple Iranian families suggests potential founder mutations. Furthermore, the evidence presented in our study underscores the importance of WES as a crucial diagnostic tool for detecting concomitant genetic disorders. - Source: PubMed
Publication date: 2025/11/11
Salami ZahraMohammadi MahsaGhasemi AidaOkhovat Ali AsgharNilipour YaldaKhani MarziehNafissi ShahriarAlavi Afagh