Ask about this productRelated genes to: HIST1H2BK Blocking Peptide
- Gene:
- HIST1H2BK NIH gene
- Name:
- histone cluster 1 H2B family member k
- Previous symbol:
- H2BFT
- Synonyms:
- H2BFAiii
- Chromosome:
- 6p22.1
- Locus Type:
- gene with protein product
- Date approved:
- 2002-11-18
- Date modifiied:
- 2016-08-15
Related products to: HIST1H2BK Blocking Peptide
Related articles to: HIST1H2BK Blocking Peptide
- The role of chromatin regulators (CRs) in mediating epigenetic changes during tuberculosis (TB) infection remains poorly understood. This study aimed to determine the efficacy of CRs in diagnosing TB and characterizing its heterogeneity. GSE83456 dataset was analyzed to identify differentially expressed CRs (DE-CRs) and immune cell infiltration in patients with TB. Consensus clustering was used to classify patients with TB based on DE-CR expression patterns. The optimal machine learning model was selected from four algorithms (Random Forest (RF), Support Vector Machine (SVM), Generalized Linear Model (GLM), and eXtreme Gradient Boosting (XGB)) to differentiate between the molecular clusters. Validation was performed using an external dataset (GSE152532). Blood samples were collected from healthy individuals and patients with pulmonary TB (PTB) or tuberculous meningitis (TBM). Analysis identified 15 DE-CRs, which were used to stratify patients with TB into two distinct molecular clusters exhibiting divergent immune microenvironment characteristics. The XGB model exhibited superior performance in distinguishing these clusters (area under the receiver operating characteristic curve = 0.965). From this model, a five-gene signature (DHRS9, HIST1H2BK, C16orf74, SLC30A1, and GBP1) was identified. This signature effectively predicted TB subtypes and was significantly associated with active TB (ATB) in an external validation set. Clinically, IFIT3 expression was validated as being significantly elevated in the blood of patients with TB (including PTB and TBM) compared to healthy controls, thereby confirming its potential role as a pan-TB biomarker. Our study revealed that CRs are closely associated with immune infiltration and heterogeneity in TB. We developed a robust XGBoost model based on a five-gene signature for accurate TB subtyping and disease-status assessment. Elevated IFIT3 expression underscores the value of CRs as novel biomarkers for TB diagnosis. - Source: PubMed
Publication date: 2025/11/25
He HuaweiWei LiuyingNong LanweiGong BeibeiXu ChaoyanZhu Qingdong - The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection triggers complex host responses, including alterations in RNA transcription and modification. Understanding these changes is crucial for elucidating viral pathogenesis and identifying potential therapeutic targets. We used direct RNA sequencing to comprehensively profile the transcriptomic and epitranscriptomic landscapes of human HEK-AT cells infected with SARS-CoV-2 at 8 h post-infection, compared to mock controls. We analysed viral and host transcriptomes, focusing on gene and transcript expression, isoform usage and RNA m6A modifications. Viral RNA sequencing reads showed 3' end-biassed coverage indicative of subgenomic RNA synthesis, with high expression of gene subgenomic RNA reads. Sixteen m6A modification sites were consistently identified in the viral genome, primarily within the and genes. In the human transcriptome, we found 254 positions with significantly altered m6A modification rates, with 119 showing decreased modification and 135 showing increased modification in infected cells. Genes with decreased m6A modifications were enriched in the neurotrophin signalling pathway. Transcript-level analysis identified 19 upregulated and 12 downregulated transcripts. Notably, transcript discovery and quantification revealed a novel isoform of the gene, which was significantly more expressed in infected cells compared to mock controls. Isoform switching analysis revealed 24 significant switches involving 21 genes, implicating mitochondrial reprogramming and immune-related pathways. In conclusion, this study provides a detailed, direct RNA sequencing-based characterization of host-virus RNA interactions, revealing key insights into SARS-CoV-2 infection mechanisms and potential therapeutic targets. - Source: PubMed
Publication date: 2025/09/17
Duru Ilhan CemPlavec ZlatkaYlinen AnneLaine PiaJames MartynRiihimäki LottaButcher Sarah JAnastasina MariaAuvinen Petri - Genomic studies of autism and other neurodevelopmental disorders have identified several relevant protein-coding and noncoding variants. One gene with an excess of protein-coding variants is that also is the gene underlying the Hypotonia, Ataxia, and Delayed Development Syndrome (HADDS). In previous work, we have identified noncoding variants in an enhancer of called hs737 and further showed that there was an enrichment of deletions of this enhancer in individuals with neurodevelopmental disorders. In this present study, we generated a novel mouse line that deletes the highly conserved, orthologous mouse region of hs737 within the Rr169617 regulatory region, and characterized the molecular and phenotypic aspects of this mouse model. This line contains a 1,160 bp deletion within Rr169617 and through heterozygous crosses we found a deviation from Mendelian expectation (p = 0.02) with a significant depletion of the deletion allele (p = 5.8 × 10). mice had a reduction of expression by 10% and mice had a reduction of expression by 20%. Differential expression analyses in E12.5 forebrain, midbrain, and hindbrain in versus mice identified dysregulated genes including histone genes (i.e., , , , and other brain development related genes (e.g., , ). phenotyping analysis (open field, hole board and light/dark transition) identified sex-specific differences in behavioral traits when comparing males versus females; whereby, males were observed to be less mobile, move slower, and spend more time in the dark. Furthermore, both sexes when homozygous for the enhancer deletion displayed body composition differences when compared to wild-type mice. Overall, we show that deletion within Rr169617 reduces the expression of and results in phenotypic outcomes consistent with potential sex specific behavioral differences. This enhancer deletion line provides a valuable resource for others interested in noncoding regions in neurodevelopmental disorders and/or those interested in the gene regulatory network downstream of . - Source: PubMed
Publication date: 2025/01/10
Hurtado Emily CordovaWotton Janine MGulka AlexanderBurke CrystalNg Jeffrey KBah IbrahimManuel JuanaHeins HillaryMurray Stephen AGorkin David UWhite Jacqueline KPeterson Kevin ATurner Tychele N - Smoking (nicotine) has been reported to possibly be neuroprotective and conducive to patients with early Parkinson's disease (PD). However, the causal effect of smoking on PD and the molecular mechanisms of smoking-related genes (SRGs) are vague. - Source: PubMed
Zhang XinyueZhu ZhigangZhu LiuhuiGuan YingZhu ZhouhaiLiu BinRen HuiYang Xinglong - Neoadjuvant chemotherapy (NACT) is routinely used to treat patients with advanced gastric cancer (AGC). However, the identification of reliable markers to determine which AGC patients would benefit from NACT remains challenging. - Source: PubMed
Publication date: 2024/06/09
Chen ZijianTang XiaochengLi WeiyaoLi TuoyangHuang JintuanJiang YingmingQiu JunHuang ZhenzeTan RongchangJi XiangLv LiYang ZuliChen Hao