Ask about this productRelated genes to: HSPA4L Blocking Peptide
- Gene:
- HSPA4L NIH gene
- Name:
- heat shock protein family A (Hsp70) member 4 like
- Previous symbol:
- -
- Synonyms:
- APG-1, Osp94, HSPH3
- Chromosome:
- 4q28.1
- Locus Type:
- gene with protein product
- Date approved:
- 2004-11-26
- Date modifiied:
- 2016-10-05
Related products to: HSPA4L Blocking Peptide
Related articles to: HSPA4L Blocking Peptide
- This study examined the effects of heat stress (HS) on mid-lactating (100 ± 14 DIM) Holstein cows. Cows were assigned to either HS (temperature-humidity index [THI] 74-80; n = 7) or thermoneutral (pair-fed PFTN; THI 64; n = 8) conditions for 4 d. Exposure to HS reduced milk (4.34 ± 1.62 kg/d), protein (0.18 ± 0.04 kg/d), and fat (0.18 ± 0.05 kg/d) yields, highlighting the negative impacts of HS on production and homeostasis. To explore main molecular responses, mammary gland tissue samples were collected for transcriptomic analysis. Transcriptomic analysis revealed 54 upregulated and 74 downregulated genes in HS conditions. Upregulated genes included heat shock proteins (, ) and other stress-responsive genes (, , ). Downregulated genes involved DNA repair ( and ) and mitochondrial function ( and ). Gene Ontology (GO) enrichment analysis indicated significant enrichment in protein quality control and stress response processes. Protein-protein interaction (PPI) of differentially expressed genes (DEG) analysis underscored networks of molecular chaperones and protein quality control pathways. Heat stress activates proteostasis-related pathways and molecular chaperone systems in the bovine mammary gland, reflecting cellular efforts to mitigate protein misfolding and maintain homeostasis. - Source: PubMed
Publication date: 2025/05/12
Perez-Hernandez GEllett M DPokhrel BParsons C L MCorl B ADaniels K M - Climate-induced stressors pose significant threats to fish growth, survival, and ecological stability. Identifying reliable molecular biomarkers is crucial for improving stress management and acclimation strategies. This study employed a comprehensive transcriptomic analysis to examine stress responses in rainbow trout (Oncorhynchus mykiss) exposed to five distinct environmental stressors-high and low temperatures, crowding, salinity, and low water quality (characterized by reduced dissolved oxygen and elevated CO)-over six hours. A total of 21,580 differentially expressed transcripts (DETs) were identified, including 16,959 unique DETs. Heat stress and salinity induced the most pronounced transcriptomic responses, with most DETs being stressor-specific, highlighting distinct physiological acclimation mechanisms. Only 39 DETs were consistently regulated across all stress conditions. Key DETs associated with heat stress were further analyzed using machine learning models to evaluate their predictive potential in distinguishing control and heat-stressed fish from natural Redband trout populations. The logistic model tree (LMT) classifier demonstrated the highest accuracy with a set of 234 DETs. When the dataset was reduced to 50 or 2 DETs, the Random Forest model achieved optimal classification, consistently identifying two heat shock protein transcripts, hsp47 and hspa4l, as primary predictors across both short- and long-term stress responses. In contrast, core DETs shared across stressors exhibited limited predictive power, achieving only 52.78% classification accuracy. These findings underscore the specificity of molecular signatures to individual stressors and highlight the potential of transcriptomic biomarkers for monitoring climate-induced stress in fish populations. The study recommends the integration of these biomarkers into selective breeding programs and conservation strategies to enhance fish resilience and welfare in the face of environmental change. - Source: PubMed
Publication date: 2025/12/20
Ali AliAli YoussefRaymo GuglielmoDalei AsutoshSalem Mohamed - Heat shock protein family A member 4-like (HSPA4L) has been shown to be overexpressed in osteoarthritis (OA) patients, but its role in OA process still unknown. Chondrocytes were stimulated with interleukin-1β (IL-1β) to mimic OA cell model in vitro, and rat was injected with monosodium iodoacetate (MIA) to construct OA rat model in vivo. The expression of HSPA4L, methyltransferase-like 3 (METTL3) and extracellular matrix (ECM)-related markers was examined by qRT-PCR or western blot. Cell proliferation and apoptosis were determined by CCK8 assay, EdU assay, TUNEL staining and flow cytometry. The levels of TNF-α and ROS were determined to assess cell inflammation and oxidative stress. The interaction between HSPA4L and METTL3 was confirmed by MeRIP assay and dual-luciferase reporter assay. Saffron-O and fast green staining was performed to evaluate cartilage degeneration in rats. HSPA4L expression was higher in OA patients and IL-1β-induced chondrocytes. Silencing of HSPA4L enhanced proliferation, while suppressed IL-1β-induced chondrocyte apoptosis, ECM degradation, inflammation and oxidative stress. METTL3 was upregulated in OA patients and IL-1β-induced chondrocytes, and it could increase HSPA4L expression by m6A modification. METTL3 knockdown inhibited IL-1β-induced chondrocyte injury, as well as alleviated cartilage degeneration in OA rat models, while these effects were reversed by HSPA4L overexpression. METTL3-mediated HSPA4L accelerated OA progression through m6A modification, providing a novel insight for OA treatment. - Source: PubMed
Chen ChenChen PingboDu ZunyuZhang JinniuZhou Yun - Hsc70Cb is essential for spermatogonia survival and sperm individualisation in Drosophila. This study highlights the conserved roles of the HSPA4 family across animals and the utility of flies as a model organism for male fertility research. - Source: PubMed
Publication date: 2025/03/07
Houston Brendan JNguyen JosephBurke RichardNogueira Alves AndréHime Gary RO'Bryan Moira K - Cryptorchidism is one of the most common reproductive abnormalities in rams, which seriously harms the reproductive capacity of rams and causes significant economic losses to the sheep industry. However, there are few studies elucidating its hereditary predisposition in sheep. - Source: PubMed
Publication date: 2025/02/19
Pei Sheng-WeiLiu Yang-KaiWang Zhong-YuYuan Ze-HuLi Wan-HongLi Fa-DiYue Xiang-Peng