Ask about this productRelated genes to: RASSF8 Blocking Peptide
- Gene:
- RASSF8 NIH gene
- Name:
- Ras association domain family member 8
- Previous symbol:
- C12orf2
- Synonyms:
- HoJ-1
- Chromosome:
- 12p12.1
- Locus Type:
- gene with protein product
- Date approved:
- 2001-04-05
- Date modifiied:
- 2016-10-05
Related products to: RASSF8 Blocking Peptide
Related articles to: RASSF8 Blocking Peptide
- The clinical significance of the long non-coding RNA (lncRNA) RASSF8-AS1 was investigated in non-small cell lung cancer (NSCLC). A comparative analysis of RASSF8-AS1 expression levels was performed in 31 paired samples of tumor tissue and adjacent morphologically normal lung tissue obtained from patients with various histological subtypes of NSCLC. Significant downregulation of RASSF8-AS1 transcript levels was observed in both lung adenocarcinoma (p = 0.0094) and squamous cell carcinoma (p = 0.0005). These findings underscore the potential of RASSF8-AS1 as a molecular marker for distinguishing histological subtypes of NSCLC. Although no significant associations were found between RASSF8-AS1 expression and patient age, tumor stage, or differentiation grade, a statistically significant correlation was identified between high RASSF8-AS1 expression and reduced overall survival specifically in patients with lung adenocarcinoma (hazard ratio, HR = 6.063; p = 0.029). This suggests that lncRNA RASSF8-AS1 may serve as a potential prognostic biomarker in this subgroup. Collectively, the results indicate a possible role for RASSF8-AS1 in NSCLC pathogenesis and support its further evaluation as both a molecular target and a prognostic tool, particularly in the context of personalized therapeutic strategies for lung adenocarcinoma. - Source: PubMed
Publication date: 2026/02/10
Kovaleva O VSinyov V VRashidova M AMalashenko O SMochalnikova V VKushlinskii N EGratchev A N - The uptake of modified lipoproteins by macrophages to form foam cells is a crucial step in atherosclerosis (AS) development. N7-methylguanosine (m7G) is frequently methylated internally in eukaryotic RNA transcripts and plays a crucial role in various processes. This study aimed to investigate the m7G RNA methylation profile in AS. We employed high-throughput sequencing to analyze the m7G methylome in foam cells induced by ox-LDL, using an in vitro AS model. Then, m7G-seq, RNA-seq, bioinformatic analysis, cell biological analyses, followed by qRT-PCR were performed. Additionally, the roles of SCARB2 and RASSF8 were investigated in an in vivo AS mouse model, and cells with SCARB2/RASSF8 overexpression/knockdown. In vitro and in vivo oil red O staining confirmed the successful establishment of the atherosclerotic foam cell and mouse models. We identified 1197 m7G peaks and 430 differentially expressed mRNAs during foam cell formation. Bioinformatics analyses revealed different m7G peaks associated with the gonadotropin-releasing hormone (GnRH) signaling pathway, cytoskeleton-dependent intracellular transport, and mitochondrial organization, regulating the processes of macrophage foaminess. Moreover, 28 key differentially expressed methylated genes were identified. m7G methyltransferases (WDR4, METTL1, WBSCR22) were upregulated in the AS cell model, and m7G modification genes (SCARB2 and RASSF8) associated with pathological processes were confirmed. Immunofluorescence staining showed that RASSF8 and SCARB2 were both expressed in AS mice plaque tissues. Finally, RASSF8/SCARB2 overexpression could promote apoptosis and lipid accumulation of ox-LDL-induced RAW264.7 cells. An m7G transcriptome-wide map of AS in vitro was created, and the differentially m7G methylated genes SCARB2 and RASSF8 may be crucial in macrophage foaminess. Our findings offer novel insights into the underlying mechanisms and potential treatments for AS. - Source: PubMed
Geng TaoFeng MengweiWang KaiyanWang HuiGu TianshuHu XiaotongLi JiaoWang HualingQi ShiyuShangguan WenfengWang WeidingZhang HaoLiu TongLiang Xue - Gastric cancer (GC) is globally recognized as one of the most widespread malignant tumors. As the symptoms of patients with early GC are ambiguous, the majority of patients are given a diagnosis of advanced GC. Therefore, this necessitates the search for new biomarkers to be utilized in the early diagnosis and screening of GC. Enhancer RNA (eRNA) is a non-coding RNA in transcription by enhancers that is tumor-specific and has a critical function in cancer progression. Our research investigates new eRNAs as bio-diagnostic markers for GC. Four eRNAs with good differential expression in GC were screened by TCGA and University of California, Santa Cruz databases. Quantitative real-time PCR was utilized for testing the level of RASSF8-AS1. The diagnostic effect of RASSF8-AS1 was evaluated using the receiver operating characteristic (ROC). Functional experiments were used to detect the ability of RASSF8-AS1 to affect the metastasis and proliferation in GC cells. The expression of RASSF8-AS1 was obviously elevated in both GC tissues and serum, whereas it was decreased in the serum levels of postoperative GC patients. ROC showed that RASSF8-AS1 was more diagnostically efficient than common diagnostic biomarkers for GC and that diagnostic effectiveness could be better than combining them. The findings of in vitro experiments showed that knocking down the level of RASSF8-AS1 clearly suppressed the ability of growth and metastasis in GC cells. Studies have shown that serum RASSF8-AS1 has the potential to contribute to the progression of GC as a biomarker for diagnosis and prognostic monitoring of GC. - Source: PubMed
Li XunChen XiaojueChen BairongGu XinliangChu XiuyuShen XianjuanJu Shaoqing - Triclosan (TCS), a fat-soluble broad-spectrum antimicrobial agent widely used in personal care products and medical disinfectants, has been linked to adverse reproductive outcomes including disrupted embryo implantation. The aim of the present study was to investigate the functional consequences and molecular mechanisms of TCS exposure on trophoblast cells, using the HTR-8/SVneo cell line as an established in vitro model of human extravillous trophoblasts. Exposure to environmentally relevant TCS concentrations (0-100 μM) revealed dose-dependent toxicity. Cell viability significantly decreased at more than 10 μM TCS, while migration was impaired at concentrations as low as 1 μM. RNA-sequencing (RNA-seq) and Chromatin immunoprecipitation sequencing (ChIP-seq) analyses of cells exposed to 10 μM TCS showed substantial redistribution of enhancer regions (marked by H3K27ac). Motif enrichment analysis identified TCF12 as the most significantly affected transcription factor among five differentially enriched factors. Further investigation demonstrated that TCS exposure upregulated both TCF12 and its target gene RASSF8. The functional relationship between these factors was confirmed through TCF12 knockdown experiments, which decreased RASSF8 expression and partially reversed TCS-induced migration inhibition. Luciferase reporter assays verified direct binding of TCF12 to the RASSF8 promoter region. This study reveals a novel TCF12-RASSF8 signaling pathway mediating TCS-induced trophoblast migration impairment, providing molecular insights into the reproductive toxicity of this common antimicrobial agent and identifying potential intervention targets for TCS-induced reproductive complications. - Source: PubMed
Publication date: 2025/08/07
Dong GuangzhuHuang WenboQin JiaheMa JinzhaoQin YufengDu Guizhen - Neuroblastoma (NB), the most common extracranial solid tumor of childhood, originates from developing sympathetic nervous system. While cuproptosis has emerged as a critical regulator in oncobiology, its mechanistic involvement in NB remains poorly characterized. - Source: PubMed
Publication date: 2025/07/01
Chen KeWang JingYang ShiminXiao JunWu LuyaoLi ZejianZhao XiangChen XuyongLi HonglinFeng JiexiongMeng Xinyao