Ask about this productRelated genes to: ATP6V0D2 Blocking Peptide
- Gene:
- ATP6V0D2 NIH gene
- Name:
- ATPase H+ transporting V0 subunit d2
- Previous symbol:
- ATP6D2
- Synonyms:
- FLJ38708, VMA6
- Chromosome:
- 8q21.3
- Locus Type:
- gene with protein product
- Date approved:
- 2002-05-09
- Date modifiied:
- 2017-03-10
Related products to: ATP6V0D2 Blocking Peptide
Related articles to: ATP6V0D2 Blocking Peptide
- Excessive osteoclast activity drives inflammatory bone loss in osteoporosis, rheumatoid arthritis, and periodontitis. Natural compounds represent promising therapeutic candidates with favorable safety profiles; however, few exhibit pathway-biased mechanisms of action. Here, we report that angelic acid (AA), a naturally occurring unsaturated monocarboxylic acid, potently inhibits RANKL-induced osteoclastogenesis. This effect occurs with an IC of 1.9 µM without cytotoxicity. Mechanistically, AA selectively suppressed RANKL-activated phosphorylation of ERK1/2, p38, and JNK (all three MAPK branches), while leaving NF-κB transcriptional activity unaffected. This preferential MAPK suppression disrupted downstream NFATc1 nuclear translocation, thereby preventing NFATc1-driven transcription of osteoclast-specific effector genes including , , and . These findings identify AA as a novel inhibitor of the RANKL-MAPK-NFATc1 axis, providing a mechanistic foundation for its therapeutic development in osteoporosis and other osteolytic diseases. - Source: PubMed
Publication date: 2026/04/17
Zhang LifangTabandeh MojtabaDeepak Vishwa - This study investigated the effects of dietary supplementation with α-mangostin (α-Ma), a bioactive xanthone derived from mangosteen pericarp, on production performance and egg quality in late-phase laying hens. The experiment was conducted using a completely randomized design. In total, 576 healthy 51-week-old Beinong No. 2 laying hens were randomly assigned to 4 dietary treatments ( = 12): a basal diet (CON) or the basal diet supplemented with 80, 120, or 160 mg/kg α-Ma. The experiment lasted for 4 weeks, after which production performance, egg quality, serum biochemical and antioxidant parameters, inflammatory markers, and uterine gene expression were evaluated. Dietary supplementation with α-mangostin, particularly at 120 mg/kg, significantly improved feed efficiency ( < 0.05), as evidenced by a reduced feed-to-egg ratio from week 2 onward, without affecting average daily feed intake or egg production rate. After 4 weeks, hens receiving 120 mg/kg α-Ma exhibited significantly greater egg weight and eggshell strength ( < 0.05). Serum and hepatic antioxidant capacities were significantly enhanced, with increased glutathione peroxidase and catalase activities, elevated total antioxidant capacity, and decreased malondialdehyde levels ( < 0.05). Moreover, α-Ma at 120 mg/kg specifically lowered the concentration of the pro-inflammatory cytokine interleukin-1β in both serum and uterine tissue ( < 0.05). At the molecular level, this dosage significantly upregulated uterine genes essential for eggshell formation ( < 0.05), including calcium transporters (, ), the matrix protein gene , and other key genes (, , , and ). In conclusion, dietary supplementation with 120 mg/kg α-Ma effectively enhances feed efficiency, strengthens antioxidant and anti-inflammatory defenses, and upregulates uterine genes involved in biomineralization, thereby improving eggshell quality in aging laying hens. These findings support α-Ma as a promising plant-based feed additive for maintaining productivity and egg quality in antibiotic-free layer production systems. - Source: PubMed
Publication date: 2026/04/05
Huang LuQin RuixinYu QianqianYan QiliQi Desheng - Hydrogen sulfide (HS) is a novel gasotransmitter produced in mammalian cells and is known to various regulate physiological functions. Previous study reported that an imbalance in HS metabolism is associated with defective bone homeostasis. However, the detailed mechanism of how HS affect osteoclast differentiation remains unclear. In the present study, we demonstrated that the effect of HS donor GYY4137 on osteoclast differentiation and multi-nucleation. Treatment of GYY4137 significantly decreased the number of receptor activator of nuclear factor kappa-B ligand (RANKL)-induced tartrate-resistant acid phosphatase (TRAP)-positive cells and inhibited the expression of osteoclast-related genes, nuclear factor of activated T-cells 1 (NFATc1) and Cathepsin K(Ctsk). Additionally, the increased gene expression of dendritic cell-specific transmembrane protein (DC-STAMP), osteoclast stimulatory transmembrane protein (OC-STAMP), and v-ATPase V0 subunit d2 (Atp6v0d2), which are cell-cell fusion-related molecules by RANKL treatment, was attenuated by GYY4137. Furthermore, GYY4137 suppressed the phosphorylation of mitogen-activated protein kinases (MAPKs), including ERK1/2, JNK1/2, and p38MAPK, compared to RANKL-treated cells. Thus, our data suggested that HS donor GYY4137 as a novel osteoclast genesis inhibitor, significantly decreases osteoclast differentiation and multi-nucleation by inhibiting the expression of the cell-cell fusion molecules. - Source: PubMed
Takagi TomohiroInoue HirofumiMorimoto HiromuTakahashi NobuyukiUehara Mariko - Heat stress significantly disrupts physiological and molecular balance in poultry, leading to oxidative damage, inflammatory responses, and metabolic dysregulation. Among emerging solutions, phytogenic adaptogens have shown promise as natural agents that enhance resilience against these environmental challenges. This exploratory study examined the transcriptomic effects of Phytocee™, a proprietary phytogenic formulation, in heat-stressed broilers, alongside in silico predictions of its phytochemical interactions with longevity-associated pathways. Phytocee™ consists of a formulation of adaptogenic medicinal plants. The primary bioactive components contributing to these adaptogenic properties include hydrolyzable tannins, withanolides, and triterpenoids. Comprehensive identification, quantification, and confirmation of these phytochemicals were conducted using liquid chromatography-mass spectrometry (LC-MS), and the formulation's integrity was validated through high-performance liquid chromatography coupled with photodiode-array detection for routine quality assurance. The transcriptomic analysis demonstrated that heat stress led to the upregulation of several vital DNA repair and cell cycle regulatory genes, including FANCF, BRCA1, and EXO1. The supplementation of Phytocee™ resulted in further increases in these genes, reaching a log2 fold change of 1.32 with a significance level of < 0.013. Additionally, resilience markers against oxidative stress such as SOD2, CAT, HSP25, HSPA2, and SOD3 along with metabolic adaptation indicators like IDH3A, ATP6V0D2, RRM2, ME1, FADS2, ALDH1L2, and DHCR7 showed significant enhancement post-treatment. There was also a restoration of several downregulated protective genes, including NFKBIA and BIRC5. DIGEP-Pred 2.0 and pathway enrichment were used in the in-silico analyses, which predicted that the key Phytocee™ phytochemicals interact with FOXO, AMPK, SIRT1, and mTOR network components. Transcriptomic patterns, such as upregulated DNA repair, oxidative resilience, and metabolic genes correlatively overlapped with this prediction. Again, no model validation or functional activation was performed. This exploratory study contributes to a hypothesis-producing framework for these associations to be tested in heat-stressed broilers but has several limitations related to the correlative nature of findings, absence of confirmation at the protein level, or functional assays, such as autophagy or pathway inhibition or direct measures of thermotolerance or production. Thus, confirmatory studies are warranted to test these implied mechanistic associations. - Source: PubMed
Publication date: 2026/03/16
Shamana KEdwin RJaishree S PPrashanth DAboli GDeepak M - Esophageal cancer (EC) remains a highly aggressive malignancy with limited therapeutic options and poor prognosis. To address the shortcomings of conventional therapies, we developed a biomimetic, reactive oxygen species (ROS)-responsive nanoprodrug for synergistic photothermal-chemotherapy of EC. Tannic acid and ellagic acid were chemically linked via boronate ester bonds to form a polyphenol-based nanoparticle (TPE). The epidermal growth factor receptor (EGFR)-targeting peptide GE11 was subsequently introduced onto red blood cell membranes (RBCM) to obtain GE11-RBCM, which was then used to cloak the TPE nanoparticles, yielding GE11-RBCM@TPE. The resulting nanoplatform exhibited excellent photothermal conversion capability under near-infrared irradiation and selectively released the therapeutic payload in response to elevated ROS levels within the tumor microenvironment. In vitro studies showed enhanced cellular uptake in EGFR-overexpressing EC cells and markedly increased cell death following combined photothermal and chemotherapeutic treatment. In vivo, GE11-RBCM@TPE significantly inhibited tumor growth with negligible systemic toxicity and prolonged blood circulation. Transcriptomic analysis further revealed up-regulation of pro-apoptotic (PER1, HK2, BMF, DAPK2) and autophagy-related genes (ATP6V0D2, HDAC10, BNIP3, DEPP1, ATG9B, NAT16), while SQSTM1 and IL6 were down-regulated, indicating simultaneous activation of apoptosis and autophagy. These findings suggest that GE11-RBCM@TPE represents a promising strategy for precise and effective treatment of esophageal cancer. - Source: PubMed
Publication date: 2026/02/27
Chen YangZhu LiXu WeiChen QiTeng FeiBao KaiwenZhang LuWang YuanWu WeiWang Zhiqiang