Ask about this productRelated genes to: STX2 Blocking Peptide
- Gene:
- STX2 NIH gene
- Name:
- syntaxin 2
- Previous symbol:
- STX2B, STX2C, STX2A, EPIM
- Synonyms:
- EPM
- Chromosome:
- 12q24.33
- Locus Type:
- gene with protein product
- Date approved:
- 1994-07-04
- Date modifiied:
- 2016-10-05
Related products to: STX2 Blocking Peptide
Related articles to: STX2 Blocking Peptide
- Rapid pathogen detection is essential during the early stages of disease treatment, as timely identification directly influences the selection of appropriate therapeutic agents. Zinc finger domains can be engineered into customizable proteins capable of binding specific target sequences in double-stranded DNA. When coupled with sequence-enabled reassembly of β-lactamase (SEER-LAC), this can provide colorimetric, sequence-specific DNA detection. The present study integrates a zinc finger protein (ZFP) array in a pump-free, capillary-driven microfluidic platform for sensitive and selective detection of the gene of O157. The zinc finger proteins are covalently bound to epoxy-functionalized surfaces, enabling robust attachment and increasing the reactive surface area-to-volume ratio. Using engineered ZFPs targeting the gene of O157, the device produces a visible yellow-to-red shift upon target binding and enzyme reconstitution, yielding a limit of detection of 1.11 pM. Specificity was confirmed against non-target and irrelevant DNA sequences, showing distinctly higher signals only for the cognate target. Flow characterization demonstrated a predictable, near-constant volumetric flow rate based on capillary wicking that was consistent with Lucas-Washburn behavior. The assay operates at ambient temperature and requires no DNA denaturation, hybridization, labeling, or amplification, reducing sample/reagent consumption and generating instrument-free readout in ∼10 min at the final stage of the ZFP array. Collectively, this work establishes a low-cost microfluidic ZFP array for direct, label-free, amplification-free detection of pathogen dsDNA, and provides a practical framework for translating programmable protein-DNA recognition into quantitative, point-of-care testing. - Source: PubMed
Publication date: 2026/04/28
Bhuiyan Shaikh Al MahmudShumway RylanTrinkle ChristineKim Moon-Soo - Culture-independent diagnostic tests (CIDT) have emerged as a preferred diagnostic approach for the rapid detection of etiologic agents of gastroenteritis. Although the relative ease and speed of CIDT support their use as a primary diagnostic test, there is still a need to isolate the organism from CIDT-positive specimens for disease surveillance. The objective of this study was to assess the performance of the testing algorithm used at the California Department of Public Health Foodborne & Waterborne Diseases Section (CDPH-FWDS) for Shiga toxin-producing Escherichia coli (STEC) recovery from CIDT-positive human stool specimens in transport medium, with particular focus on molecular screening of enrichment broths for a Shiga toxin gene (stx). When STEC was not isolated by culture of the submitted stool specimen, CDPH-FWDS subcultures from an enrichment broth started from the stool specimen. Enrichment broth can improve STEC recovery by allowing target bacteria to multiple while limiting the growth of normal fecal flora. The enrichment broths were screened for stx1 and stx2 using a lab developed real-time PCR assay. STEC was isolated from only 3% of the enrichment broths that were negative for stx1 and stx2 by molecular screening. This work demonstrates the utility of molecular screening of enrichment broths prior to culturing for bacterial isolation. Discontinuation of culture from stx molecular screen negative enrichment broths will result in decreased hands-on laboratory testing time and cost savings with minimal impact on STEC surveillance efforts. - Source: PubMed
Publication date: 2026/04/18
Patterson QuinnKirvida-McGowan TrinityZhao YueqingShiozaki MariceZhu JonathanKaneko BeverleyAbromaitis Stephanie - Shiga toxin-producing (STEC) O26 is increasingly implicated in human hemolytic uremic syndrome globally. As cattle are an important reservoir, we conducted a year-long pilot study on a dairy farm in England (~200 cattle) to investigate the occurrence, persistence, and genomics of STEC O26. - Source: PubMed
Publication date: 2026/04/09
Deeney Alannah SRaj Kumari SanjuktaWithenshaw Susan MBare Harriet LDuggett Nicholas AKirchner MirandaSmith Richard PDavies Robert HRodgers John DAnjum Muna F - In January 2025, Santé publique France detected several cases of hemolytic uremic syndrome (HUS) in adults caused by a rare serotype of Shiga toxin-producing , STEC O77g:H18. Epidemiological and traceability investigations linked the consumption of raw-milk cheese to these cases. The outbreak strain belonged to sequence type 69 (ST69) and harbored a Shiga toxin (stx) subtype located on a unique prophage. Putative virulence genes , , , and were detected; however, STEC virulence genes ( and ) were absent. Phylogenetic analysis of O77g strains from public databases (Enterobase and NCBI) placed the outbreak strain within a cluster of clinical O77g:H18, ST69, strains isolated between 2012 and 2025, including one linked to a HUS case in France and three in Australia. This strain, distinct from O77g:H18 strains previously isolated from dairy products, is therefore not a new lineage. The closest cluster contains eight -negative strains isolated from poultry between 1990 and 2024 in Europe and North America. Differences in virulence gene composition between the "outbreak cluster" and the "poultry cluster" suggest a common ancestor that has diverged over time. The "outbreak cluster" is characterized by strains carrying an IncFIA/FIB plasmid encoding a putative enterotoxin gene () absent in other strains in GenBank. We developed qPCR assays specific for the putative enterotoxin and O77g O-group. Combined with a PCR for , these assays allow rapid screening of suspect unpasteurized cheese for the outbreak strain.IMPORTANCEThe emergence of hybrid pathotypes of Shiga toxin-producing (STEC) has been of importance in recent years in public health. This outbreak, related to a rare serotype of STEC and involving an enterotoxigenic (ETEC)/STEC hybrid, highlights the necessity of phylogenetic analyses to investigate the origin of contamination with such strains. Our study suggests a common ancestor between the outbreak strain and strains isolated from poultry. We also provided molecular tools to target specifically this outbreak strain and help in future epidemiological investigations. - Source: PubMed
Publication date: 2026/04/24
Vorimore FabienTran Mai-LanCointe Auréliede Larminat JustineBidet PhilippeBonacorsi StéphaneSilva Nodari CarolinaWeill François-XavierFach PatrickDelannoy Sabine - Alkaptonuria is a rare inborn error of metabolism, with few reports from Brazil and typically lacking genotype descriptions in the Brazilian population. - Source: PubMed
Publication date: 2026/01/06
Moreno Carolina Araújode Oliveira Sobrinho Ruy PiresJorente JosepAppenzeller SimoneMeira Nelma Gláucia SilvaCosta Lucas Cadete CaldeiraAcosta Angelina Xavier Guaragna Mara SanchesSteiner Carlos Eduardo