TRF3 Blocking Peptide
- Known as:
- TRF3 Blocking Peptide
- Catalog number:
- 33r-2922
- Product Quantity:
- USD
- Category:
- -
- Supplier:
- Fitzgerald industries international
- Gene target:
- TRF3 Blocking Peptide
Related genes to: TRF3 Blocking Peptide
- Gene:
- TBPL2 NIH gene
- Name:
- TATA-box binding protein like 2
- Previous symbol:
- -
- Synonyms:
- TRF3, TBP2
- Chromosome:
- 14q22.3
- Locus Type:
- gene with protein product
- Date approved:
- 2003-01-13
- Date modifiied:
- 2016-10-05
Related products to: TRF3 Blocking Peptide
Related articles to: TRF3 Blocking Peptide
- Breast cancer is the leading cause of death among women globally. Several genes have been found to be transcriptionally dysregulated in cancer, according to recent studies. TATA-box binding protein (TBP) and its two paralogs, TBPL1 and TBPL2, play roles in human transcription. The TBPL1 gene is implicated in colorectal carcinomas by suppressing the expression of miR-18a. However, its function in breast cancer remains undisclosed. TBPL1 is distantly related to TBP and possesses a 40% similarity with TBP's core domain. In the present study, we explored the potential role of the TBPL1 gene in transcriptome regulation by knocking out the TBPL1 gene through the CRISPR/Cas9 method. Following the knockout of the TBPL1 gene, we examined the gene transcription patterns and compared them to wild-type cell lines. We observed disparate signatures of upregulated and downregulated genes in wild-type and mutated conditions. Healthy breast MCF-12F, and T47D, SKBR3, and MDA-MB-231 breast cancer cell lines were assessed, as these cancer cells exhibit overexpression of the TBPL1 gene. Next-generation sequencing data revealed distinct marker genes regulated by the TBPL1 gene and their potential involvement in cell migration, proliferation, anti-apoptosis, and metastasis. Additionally, we also discovered novel lncRNAs implicated in the transcriptome analysis of the TBPL1 knocked-out gene. Our investigation indicated that this gene might affect varied stages of breast cancer cell lines' cellular properties, such as cell duplication, morphology, and growth. It might also contribute to tumor formation in more aggressive cell lines like MDA-MB-231 in vivo. - Source: PubMed
Publication date: 2025/10/28
Mishal RabiaMeléndez-Zajgla JorgeRueda-Zarazúa BerthaLabra-Barrios María LuisaCastañón-Sánchez Carlos AlbertoUribe Carvajal SalvadorPadierna-Mota LauraHernández-Hernández José ManuelLeon-Avila GloriaPérez Rangel ArmandoHernández-Martínez EdgarAngeles-Morales Erika BeatrizAlbalawi Ibrahim KhalilLuna-Arias Juan Pedro - To investigate the genetic factors underlying diminished ovarian reserve (DOR) and premature ovarian insufficiency (POI) in 55 infertile women of reproductive age in China and to evaluate the outcomes of assisted reproductive technology (ART) treatment in cases of genetically associated DOR/POI. - Source: PubMed
Publication date: 2025/09/11
Xu QianhuaDing HaitianLiu YingchunHan DanXia XunLi YuqianSha XuanLi GuotongNi XiaoqingLi KuokuoHua RongHe XiaojinWu HuanCao YunxiaXu Yuping - Female infertility is a common and complex health problem affecting millions of women worldwide. While multiple factors can contribute to this condition, the underlying cause remains elusive in up to 15%-30% of affected individuals. In our large genome-wide association study (GWAS) of 22,849 women with infertility and 198,989 control individuals from the Finnish population cohort FinnGen, we unveil a landscape of genetic factors associated with the disorder. Our recessive analysis identified a low-frequency stop-gained mutation in TATA-box binding protein-like 2 (TBPL2; c.895A>T [p.Arg299Ter]; minor-allele frequency [MAF] = 1.2%) with an impact comparable to highly penetrant monogenic mutations (odds ratio [OR] = 650, p = 4.1 × 10). While previous studies have linked the orthologous gene to anovulation and sterility in knockout mice, the severe consequence of the p.Arg299Ter variant was evidenced by individuals carrying two copies of that variant having significantly fewer offspring (average of 0.16) compared to women belonging to the other genotype groups (average of 1.75 offspring, p = 1.4 × 10). Notably, all homozygous women who had given birth had received infertility therapy. Moreover, our age-stratified analyses identified three additional genome-wide significant loci. Two loci were associated with early-onset infertility (diagnosed before age 30), located near CHEK2 and within the major histocompatibility complex (MHC) region. The third locus, associated with late-onset infertility, had its lead SNP located in an intron of a long non-coding RNA (lncRNA) gene. Taken together, our data highlight the significance of rare recessive alleles in shaping female infertility risk. The results further provide evidence supporting specific age-dependent mechanisms underlying this complex disorder. - Source: PubMed
Publication date: 2024/11/19
Ruotsalainen SanniKarjalainen JuhaKurki MitjaLahtela ElisaPirinen MattiRiikonen JuhaRitari JarmoTammi SiljaPartanen JukkaLaivuori Hannele Palotie AarnoHeyne HenrikeDaly MarkWiden Elisabeth - In recent years, an increasing number of genes associated with male and female infertility have been identified. The genetics of infertility is no longer limited to the analysis of karyotypes or specific genes, and it is now possible to analyse several dozen infertility genes simultaneously. Here, we present the diagnostic activity over the past two years including 140 patients (63 women and 77 men). Targeted sequencing revealed causative variants in 17 patients, representing an overall diagnostic rate of 12.1%, with prevalence rates in females and males of 11% and 13%, respectively. The gene-disease relationship (GDR) was re-evaluated for genes due to the addition of new patients and/or variants in the actual study. Five genes changed categories: two female genes (MEIOB and TBPL2) moved from limited to moderate; two male genes (SOHLH1 and GALNTL5) moved from no evidence to strong and from limited to moderate; and SEPTIN12, which was unable to classify male infertility, was reclassified as limited. Many infertility genes have yet to be identified. With the increasing integration of genetics in reproductive medicine, the scope of intervention extends to include other family members, in addition to individual patients or couples. Genetic counselling consultations and appropriate staffing will need to be established in fertility centres. Trial registration number: Not applicable. - Source: PubMed
Publication date: 2024/04/25
Okutman ÖzlemGürbüz Ali SamiSalvarci AhmetBüyük UmutRuso HalilGürgan TimurTarabeux JulienLeuvrey Anne-SophieNourisson ElsaLang CécileMuller JeanViville Stephane - The understanding of the mechanisms that regulate gene expression to establish differentiation programs and determine cell lineages, is one of the major challenges in Developmental Biology. Besides the participation of tissue-specific transcription factors and epigenetic processes, the role of general transcription factors has been ignored. Only in recent years, there have been scarce studies that address this issue. Here, we review the studies on the biological activity of some TATA-box binding protein (TBP)-associated factors (TAFs) during the proliferation of stem/progenitor cells and their involvement in cell differentiation. Particularly, the accumulated evidence suggests that TAF4, TAF4b, TAF7L, TAF8, TAF9, and TAF10, among others, participate in nervous system development, adipogenesis, myogenesis, and epidermal differentiation; while TAF1, TAF7, TAF15 may be involved in the regulation of stem cell proliferative abilities and cell cycle progression. On the other hand, evidence suggests that TBP variants such as TBPL1 and TBPL2 might be regulating some developmental processes such as germ cell maturation and differentiation, myogenesis, or ventral specification during development. Our analysis shows that it is necessary to study in greater depth the biological function of these factors and its participation in the assembly of specific transcription complexes that contribute to the differential gene expression that gives rise to the great diversity of cell types existing in an organism. The understanding of TAFs' regulation might lead to the development of new therapies for patients which suffer from mutations, alterations, and dysregulation of these essential elements of the transcriptional machinery. - Source: PubMed
Publication date: 2023/12/21
Luna-Arias Juan PedroCastro-Muñozledo Federico