Ask about this productRelated genes to: TRPM5 Blocking Peptide
- Gene:
- TRPM5 NIH gene
- Name:
- transient receptor potential cation channel subfamily M member 5
- Previous symbol:
- -
- Synonyms:
- LTRPC5, MTR1
- Chromosome:
- 11p15.5
- Locus Type:
- gene with protein product
- Date approved:
- 2002-01-11
- Date modifiied:
- 2016-01-28
Related products to: TRPM5 Blocking Peptide
Related articles to: TRPM5 Blocking Peptide
- Proteins operate in dynamic environments where ions, lipids and temperature collectively define their properties, yet most studies rely on simplified conditions that overlook these intrinsic variables. Here we show two such factors-temperature and Ca-remodel the function and pharmacology of TRPM4, an ion channel implicated in cardiac conduction, immune regulation, cancer and intestinal-fluid homeostasis. At physiological temperature and Ca, TPPO-previously considered a selective TRPM5 inhibitor inactive toward TRPM4-potently activates TRPM4, revealing strong synergy among temperature, Ca and ligand binding. By contrast, Necrocide-1, a necroptotic activator targeting the same binding pocket, defies this logic: it opens TRPM4 without Ca but is antagonized by Ca. Meanwhile, the inhibitors NBA and CBA engage a nearby pocket, locking the channel in a non-conductive pre-open state. Our findings highlight that even rigid binding pockets can exhibit temperature-dependent ligand recognition, revealing hidden pharmacology and informing selective, environment-aware therapeutic strategies. - Source: PubMed
Publication date: 2026/06/09
Hu JinhongIevleva SofiaPark Sung JinLee JunukCheng JieO'Dea GarrettSheng JiangnanDu JuanLü Wei - Water-soluble soybean fiber (WSSF) exerts health benefits beyond its fermentability, but its direct effects on epithelial-immune interactions remain unclear. We investigated whether WSSF regulates antimicrobial proteins (AMPs) in the small intestine and the underlying mechanisms. C57BL/6J and tuft cell-deficient Pou2f3-knockout mice were fed control or WSSF-supplemented diets, and gene and protein expression and cecal microbiota were analyzed. WSSF upregulated the epithelial AMPs SPRR2A, RELMβ, and ANG4 in the jejunum and ileum. Pharmacological inhibition of group 2 innate lymphoid cells (ILC2) or TRPM5 attenuated AMP induction and reduced IL-25, IL-13, tuft cell markers, and STAT6 phosphorylation. WSSF also failed to induce AMPs or type 2 immune markers in -knockout mice, indicating a requirement for tuft cells. Finally, WSSF altered cecal microbiota composition in a tuft cell-dependent manner. These findings identify WSSF as a soybean-derived fiber that strengthens epithelial innate defense via a TRPM5-tuft cell-ILC2 axis. - Source: PubMed
Publication date: 2026/06/03
Ahmad ArslanWijatniko Bambang DwiMapure Siueia Cláudia DomingosYanagi ChisatoIshii YoshikiIshikawa ShodaiInoue RyoRini Dina MustikaYanaka NoriyukiSuzuki Takuya - is an important source of steviol glycosides with antidiabetic properties. This study evaluated the effects of abiotic elicitors, including foliar application of 5% ethanol and root-zone excess iron (150 mg/L Fe; +Fe), on stevioside accumulation and the biological activity of leaf extracts in pancreatic β-cells. Ethanol treatment significantly stimulated vegetative growth, resulting in an approximately threefold increase in fresh biomass and a 62% enhancement in photosynthetic rate, without inducing oxidative stress. HPLC analysis demonstrated that stevioside content increased by 69% in ethanol-treated plants and by 104% under + Fe conditions, while rebaudioside A remained unchanged. In MIN-6 β-cells, ethanol-derived extracts upregulated expression by 3.11-fold at 12 h and triggered strong early activation of (20-fold at 2 h), followed by delayed induction. In contrast, +Fe extracts showed weaker effects on insulin-related genes. Overall, ethanol acts as an efficient elicitor enhancing stevioside biosynthesis and insulinotropic potential . - Source: PubMed
Publication date: 2026/04/28
Seifi FatemehMehrabanjoubani PooyanBarzegar AliBiparva PouryaSajadi Kaboudi Parvin - Estrogen deficiency not only induces osteoporosis in long bone but also promotes inflammatory alveolar bone resorption. Increasing evidence indicated the role of the gut-bone axis in estrogen deficiency-aggravated bone loss. Berberine is a Traditional Chinese Medicine with known gut microbiota modulating activity and exhibits potential in the prevention and treatment of bone resorption. - Source: PubMed
Publication date: 2026/03/09
Du QianZhang ZhiyingTu YeZheng XinPeng XianLiu RuiXie QianZhou XuedongZheng LiweiXu Xin - This research conducts a systematic exploration of the impacts of three fixation methods-50-type electric heating tube roller fixation (CQ), mixed fixation (HC), and steam fixation (ZQ)-on the quality formation of green tea. By means of quantitative analysis of aroma components, catechins, free amino acids, and alkaloids, in combination with electronic tongue taste fingerprint and untargeted metabolomics analysis, it is discovered that distinct fixation methods exert significant influences on the volatile and non-volatile metabolite profiles of green tea. CQ notably elevated the content of total polyphenols and gallic acid to 3.57 mg/g, intensifying its bitter and astringent taste profile. In contrast, ZQ produced the brightest green infusion and yielded the highest electronic tongue response value for sweetness. HC achieved a superior balance, retaining the highest levels of key aroma compounds like linalool (1.42 mg/kg) and D-limonene, while also preserving the highest total free amino acid content (13,983.2 µg/g). Metabolomics analysis further disclosed that fixation methods affect metabolite composition by regulating pathways such as phenylpropanoid biosynthesis and caffeine metabolism. Molecular docking experiments verify that the disparity in binding affinity between key differential metabolites (e.g., Chrysophanol 8-(6-galloylglucoside)) and taste receptors (TRPM5, TAS2R14, T1R1/T1R3) serves as the material foundation for taste differentiation. This study provides a theoretical basis for the development of precision processing technologies and quality control of green tea. PRACTICAL APPLICATIONS: This study provides a decision-making framework for green tea producers to select fixation techniques based on desired sensory profiles. To enhance floral and citrus notes, mixed fixation (HC) is recommended. Steam fixation (ZQ) is ideal for preserving a bright green color and sweeter taste, suitable for premium-grade teas. Roller fixation (CQ) can be applied where a stronger, more astringent profile is targeted. These insights enable the precision manufacturing of green teas with customized flavor characteristics. - Source: PubMed
Xiong LiZhou YangyuPeng RuishuXiao YueHuang YinaGao Hong