Ask about this productRelated genes to: REG3A Blocking Peptide
- Gene:
- REG3A NIH gene
- Name:
- regenerating family member 3 alpha
- Previous symbol:
- PAP
- Synonyms:
- HIP, REG-III, REG3, PBCGF, PAP1
- Chromosome:
- 2p12
- Locus Type:
- gene with protein product
- Date approved:
- 1993-09-20
- Date modifiied:
- 2015-11-18
Related products to: REG3A Blocking Peptide
Related articles to: REG3A Blocking Peptide
- Dysbiosis after allogeneic hematopoietic stem cell transplant (allo-HSCT) predisposes to acute gastrointestinal (GI) graft-versus-host disease (GVHD). Human milk oligosaccharides (HMOs) are prebiotics that establish a favorable intestinal microbiome. We hypothesized that peri-transplant HMOs would reduce dysbiosis, and conducted a prospective phase I/II study (NCT04263597 Clinicaltrials.gov) in which 70 patients received oral, daily 2'-fucosyllactose (2'-FL), the most abundant HMO, from start of conditioning through day +30. Primary phase I and phase II endpoints were safety and tolerability of 2'-FL and longitudinal preservation of microbiome diversity, respectively. Phase I and phase II patients with ³60% adherence were evaluated by correlative analyses. Twenty-nine age-matched patients with available samples in our institutional biorepository served as contemporary controls. 2'-FL was safe and well-tolerated on the phase I study, which established dosing for the phase II study. Shannon diversity was preserved in 2'-FL recipients and declined from baseline to day +30 in controls (p=0.04). Generalized linear mixed model analysis revealed higher association of Bifidobacteria and Blautia with 2'-FL (adjusted p<0.05). Higher fecal isoleucine (p=0.005), tyrosine (p=0.05), and taurine (p=0.04) were observed in 2'-FL recipients, all associated with protection and regeneration of intestinal epithelium. Lower plasma reg3a (p=0.053) and ST2 levels (p=0.038) were observed in 2'-FL recipients compared to controls. Incidence of grade II-IV acute GI GVHD was 0% in 2'-FL recipients compared to 17% in controls (p=0.009). Adenovirus reactivation was 0% in 2'-FL recipients compared to 17% in controls (p=0.009). 2'-FL is safe and modifies the gut microbiome in children following allo-HSCT. - Source: PubMed
Publication date: 2026/08/11
Ramos Kristie NLangenberg LucilleLake Kelly EHoerth Colin AndrewLuebbering NathanHaslam David BNewburg DavidMorrow Ardythe LOllberding Nicholas JTeusink-Cross AshleyTaggart CynthiaBota KylieCook EleanorKhoury RubyDenson Lee AWatanabe-Chailland MikiRomick LindseyDavies Stella MKhandelwal Pooja - Human immunodeficiency virus (HIV) infection causes gut epithelial barrier dysfunction and dyslipidemia. Intestinal fatty acid-binding protein (I-FABP) and regenerating islet-derived protein 3 alpha (REG3α) are markers of gut barrier integrity. We investigated the association between these biomarkers and LDL-cholesterol (LDL-C) in HIV-infected individuals, stratified by immune status and disease duration. This cross-sectional study included 69 HIV-infected adults on antiretroviral therapy. Plasma I-FABP, REG3α, and lipid parameters were measured. Associations were evaluated using Spearman correlation in the total cohort and stratified by cluster of differentiation 4 (CD4) count (<350, 350-500, >500 cells/μL) and disease duration (≤24, 24-48, >48 months). In the total cohort (n = 50 with lipid data), neither I-FABP (rs = -0.044, P = .762) nor REG3α (rs = 0.232, P = .105) showed a significant correlation with LDL-C. However, in patients with CD4 < 350 cells/μL (n = 12), REG3α demonstrated a strong positive correlation with LDL-C (rs = 0.643, P = .024). This association was absent in patients with higher CD4 counts (350-500: rs = 0.393, P = .383; >500: rs = 0.003, P = .985). No significant associations were observed across disease duration strata. REG3α correlates with LDL-C specifically in immunocompromised HIV patients (CD4 < 350 cells/μL), suggesting that gut barrier dysfunction may influence lipid metabolism preferentially in advanced HIV disease. This finding warrants further investigation regarding cardiovascular risk stratification in this vulnerable population. - Source: PubMed
Evik GulizUcdal MeteErsoz Gulden - Regenerating islet-derived protein 3-alpha (REG3A), a member of the regenerating islet-derived protein (Reg) family, belongs to the C-type lectin class of antimicrobial peptides (AMPs). It has been recognized as a critical component of intestinal mucosal barrier damage and repair. In a prospective cohort (82 ulcerative colitis [UC] patients, 20 controls), UC patients were stratified by clinical partial Mayo Score (pMS) and endoscopic activity. Fecal REG3A levels were measured across distinct patient cohorts, and diagnostic performance was evaluated and compared against fecal calprotectin (FC) and a combined model using receiver operating characteristic (ROC) analysis. REG3A expression was also evaluated in intestinal biopsies via immunohistochemistry. Fecal REG3A levels increased stepwise with UC clinical severity (p < 0.001), peaking in the severe activity group. A combined model of REG3A and FC outperformed either biomarker alone in differentiating disease activity, achieving the highest area under the curves (AUCs) for distinguishing remission from mild activity and mild from moderate activity. Immunohistochemistry confirmed REG3A expression in active UC colonic tissues. Our results indicate that fecal REG3A serves as a promising biomarker for assessing disease activity in UC. Its combination with FC enhances the accuracy of predicting clinical severity in UC. - Source: PubMed
Lv XinruiQi YaxinGuo JiaBa LingWang SipuCao HailongXu Xin - Real-time profiling of plant volatiles provides insight into metabolic regulation, yet rapid and high-throughput characterization remains analytically challenging. Here, we apply temperature programming secondary electrospray ionization (TP-SESI) mass spectrometry to compare volatile organic compound (VOC) profiles from wild-type and PAP1-overexpressing tobacco (Nicotiana tabacum). This study extends the application of TP-SESI, which was originally developed for plant volatile analysis, to evaluate genotype-specific metabolic differences. TP-SESI enabled sensitive detection of VOCs and semi-volatile compounds spanning three major plant volatile classes: terpenoids, phenylpropanoids/benzenoids, and fatty acid derivatives, across both genotypes. While the two genotypes shared many detected features, PAP1-overexpressing plants were associated with upregulation of these indicated VOCs, suggesting broad metabolic reprogramming associated with PAP1 activation. Statistical analyses revealed clear separation between genotypes, demonstrating TP-SESI captures robust, reproducible metabolic differences in intact plant tissue. These results suggest that TP-SESI is capable of detecting genotype-associated variations in plant volatile emissions and may be useful for examining transcription factor-related changes in plant metabolism. - Source: PubMed
Publication date: 2026/06/20
Ashbacher Sarah MXie De-YuMuddiman David C - Arabidopsis MVK negatively regulates sucrose-induced anthocyanin accumulation by modulating SUC1-mediated sucrose transport and gibberellin homeostasis. Anthocyanins are flavonoid pigments that function as crucial modulators of plant responses to environmental stressors by mitigating oxidative damage and facilitating cellular adaptation. Anthocyanin biosynthesis is tightly regulated by transcriptional networks that respond to developmental cues and external stimuli. In this study, we identify mevalonate kinase (MVK), a critical enzyme in the cytosolic isoprenoid biosynthesis pathway, as a repressor of sucrose-induced anthocyanin production in arabidopsis. Loss-of-function mvk-1 mutants show increased anthocyanin levels compared to wild-type (WT) plants under high-sucrose conditions. The expression of anthocyanin biosynthetic and regulatory genes, such as CHS, DFR, and MYB75/PAP1, increases in mvk-1 mutants grown in the presence of high sucrose, and mvk-1 mutants exhibit elevated sucrose accumulation through the upregulation of sucrose transporters compared to WT under high-sucrose conditions. Furthermore, reduced gibberellic acid (GA) in mvk-1 mutants resulted in stabilization of repressors of GA signaling, known as DELLA proteins, thereby facilitating sucrose-induced anthocyanin accumulation. Our findings suggest that MVK negatively regulates sucrose-induced anthocyanin biosynthesis by modulating sucrose transport and GA homeostasis in arabidopsis. - Source: PubMed
Publication date: 2026/06/15
Kang JinkuCho SuaKang KiyoonKim DaewonBae Sang-IlShin EunjiPark So-YonStacey GaryPaek Nam-ChonCho Sung-Hwan