Ask about this productRelated genes to: SFRP2 Blocking Peptide
- Gene:
- SFRP2 NIH gene
- Name:
- secreted frizzled related protein 2
- Previous symbol:
- -
- Synonyms:
- SARP1, SDF-5, FRP-2
- Chromosome:
- 4q31.3
- Locus Type:
- gene with protein product
- Date approved:
- 1998-07-15
- Date modifiied:
- 2016-05-04
Related products to: SFRP2 Blocking Peptide
Related articles to: SFRP2 Blocking Peptide
- Dental pulp homeostasis and regeneration rely on specialized mesenchymal populations, yet aging promotes fibrotic remodeling and functional decline. Here, using the continuously growing mouse incisor as an experimentally tractable model, we integrate single-cell RNA sequencing, spatial transcriptomics, lineage tracing, and functional assays to investigate age-associated pulp remodeling. We identify a distinct Lypd1 fibroblast progenitor population that is particularly vulnerable to age-associated dysfunction. Lineage tracing and trajectory analysis show that these cells originate from the Sfrp2 stem cell pool, while functional studies of MACS-isolated human LYPD1 cells provide supportive evidence for a related progenitor-like state. Notably, early aging preferentially affects Lypd1 progenitors, rather than Sfrp2 stem cells, promoting fibrotic conversion through aberrant upregulation of 11β-HSD2, which enhances aldosterone-mediated mineralocorticoid receptor (MR)-associated signals. Additionally, aging promotes the accumulation of Ccl4 macrophages that establish a pro-inflammatory niche, where macrophage-derived PDGFB induces 11β-HSD2 via the P38 MAPK signaling. In vivo inhibition of the PDGF/P38/11β-HSD2 axis attenuates age-related pulp fibrosis. Together, our findings indicate that age-associated pulp fibrosis in this model is driven, at least in part, by niche-mediated metabolic reprogramming of progenitor cells rather than intrinsic stem cell exhaustion, highlighting corticosteroid metabolism as a potential therapeutic target for fibrotic degeneration in aging dental pulp. - Source: PubMed
Publication date: 2026/07/07
Yu XiaoyiSun TianmengXu MengyuZhao TianyuanLuo HuanyuZhong QingLiu CangweiAn Zhengwen - Keloids are benign fibroproliferative skin lesions characterized by excessive collagen deposition and growth beyond the original wound margins. However, the cellular heterogeneity and molecular mechanisms underlying keloid pathogenesis remain incompletely understood. Publicly available single-cell RNA sequencing (scRNA-seq) data from keloid tissues of eight patients were obtained from the NCBI Gene Expression Omnibus (GEO) database. High-dimensional weighted gene co-expression network analysis (hdWGCNA) was performed to identify disease-associated gene modules. Mendelian randomization (MR) analysis was performed using summary statistics from BioBank Japan to evaluate potential associations between gene expression and keloid susceptibility. ATAC-seq data were used to assess chromatin accessibility, and SCENIC analysis was performed to infer transcription factor regulon activity. Correlation analysis was then used to assess potential associations between candidate transcription factors and target genes. We analyzed 73,790 cells and identified nine major cell types. Fibroblasts showed substantial heterogeneity and were further classified into 6 subpopulations. Trajectory analysis suggested a differentiation continuum among disease-associated fibroblasts, and cell-cell communication analysis indicated that MSC-like fibroblasts may function as a signaling hub. hdWGCNA identified a turquoise co-expression module significantly enriched in MSC-like fibroblasts. MR analysis highlighted CLEC2B, MGST3, and SFRP2 as candidate genes potentially associated with keloids, and qRT-PCR validated their differential mRNA expression between normal dermal and keloid fibroblasts. ATAC-seq analysis identified 4,607 differentially accessible chromatin peaks. Integrated motif enrichment and SCENIC analyses identified CREB3L1 and ZEB1 as candidate transcription factors. Correlation analysis further suggested potential regulatory relationships between these transcription factors and the candidate genes. This study provides an integrative multi-omics characterization of cellular heterogeneity in keloids. It identifies CLEC2B, MGST3, and SFRP2 as key candidate genes, as well as CREB3L1 and ZEB1 as candidate transcription factors potentially involved in keloid pathogenesis. These findings improve our understanding of keloid biology and may provide a basis for future therapeutic research. - Source: PubMed
Publication date: 2026/07/07
Luo QianqianZhu XianweiZhou HuihuiWang ChunleiSu Yongtao - Compared to lean pig genotypes, the local Krškopolje pig is characterised by a greater capacity for lipid and lower capacity for protein deposition, along with lower dietary protein requirements. The aim of the present study was to unravel the transcriptomic differences in subcutaneous adipose tissue between the local Krškopolje pig and modern hybrids, as well as to study the impact of the reduced protein diet in both genotypes. Differential gene expression analysis between local and modern pigs revealed 375 differentially expressed genes, with 189 upregulated and 186 downregulated in Krškopolje pig. Among the upregulated genes, several suggested more pronounced adipogenesis (SLC7A10, ADIRF, INHBB, and SFRP2) in the Krškopolje pig. In addition, genes encoding collagen proteins and extracellular matrix remodelling (e.g., COL6A5, COL4A5, COL2A1), calcium signalling (TRPV4, CAMK2A, CALML5), pro-inflammatory cytokines (e.g., IL1A, TNFSF9, CXCL8, PTGS2 etc.) and cholesterol metabolism (CYP1A1, CYP2A19, CES1 etc.) were differentially expressed. The reduced protein diet induced only minor changes in gene expression at the individual gene level. However, gene set enrichment analysis revealed that, in Krškopolje pigs, dietary effects were associated with biological processes related to immune response, intracellular protein transport, and extracellular matrix organisation. In the case of modern hybrids, a reduced protein diet resulted in enrichment of gene sets related to oxidative phosphorylation, mitochondrial function, and energy metabolism. In conclusion, this study demonstrated that the adipose tissue of Krškopolje pigs compared with modern hybrids exhibited greater adipogenic potential, being also supported by higher expression of genes involved in extracellular matrix remodelling and inflammation. The impact of a reduced protein diet on adipose-tissue gene expression was relatively small in both breeds, particularly in Krškopolje pig, suggesting a metabolic adaptability of this breed. - Source: PubMed
Publication date: 2026/05/27
Poklukar KČandek-Potokar MSavić BŠkrlep M - Granulosa cells (GCs), an element of the ovarian follicle, are crucial for oocyte maturation, folliculogenesis, and steroidogenesis. Granulosa cells play a crucial role in fertilization by providing metabolic and hormonal support to the oocyte, maintaining its quality and regulating its meiotic arrest. Oocyte quality and fertilization efficiency depend on the proper activity of GCs, especially their mutual communication, providing metabolic support and protecting against oxidative stress. When interrupted, they may take part in the pathogenesis of polycystic ovary syndrome, premature ovarian failure, primary ovarian insufficiency, and diminished ovarian reserve. GCs are enclosed in the antrum where they communicate with surrounding cells, create a dynamic microenvironment, and regulate hormone biosynthesis. To analyze molecular mechanisms regulating endogenous signaling, it is important to consider the dynamic transcriptomic response of porcine GCs during in vitro culturing over 48, 96, and 144 h. Transcriptomic analysis revealed a variable and dynamic transcriptional upregulation of genes associated with cellular response to endogenous and external stimuli, chemical compound metabolism, vascular development, and GCs migration. Also, proven by Gene Ontology (GO) enrichment analysis, the following terms were highlighted: "cellular response to chemical stimulus" and "cellular response to organic substance". Specific genes, such as , , , , , , , and , exhibited significant expression changes, suggesting extensive GCs self-regulation and metabolism changes. Further analysis indicates improvements in cellular response to a cytokine stimulus, growth factor response, hormone response, enzyme-linked receptor protein signaling, and positive regulation of cell migration. These findings suggest interweaving of regulatory mechanisms underlying intercellular communication in GCs during in vitro culturing, despite the lack of signals from the native ovarian environment. Further investigating interplays of detecting pathways will provide a more comprehensive understanding and even insights into the potential clinical use of the knowledge about the role of GCs in folliculogenesis, oocyte maturation and ovulation. - Source: PubMed
Publication date: 2026/06/16
Data KrzysztofKranc WiesławaBlatkiewicz MałgorzataJózkowiak MałgorzataKulus MagdalenaKulus JakubGnus MichałDomagała DominikaChmielewski Piotr PawełKałuża AnnaŻok AgnieszkaNiebora JuliaBryja ArturOlechnowicz AnnaPiotrowska-Kempisty HannaMozdziak PaulKempisty BartoszAntosik PawełBukowska DorotaSkowroński Mariusz T - Ovarian cancer is a highly lethal gynecologic malignancy, with metabolic reprogramming being a key contributor to its progression and therapeutic resistance. Although Secreted Frizzled-Related Protein 2 (SFRP2) has been implicated in various cancers, its functional role and molecular mechanisms in ovarian cancer, particularly in regulating metabolic pathways, remain poorly defined. - Source: PubMed
Publication date: 2026/06/23
Sheng JindongXing YiwenLuan JingLiu XiangyuSong HualinSun LuYu MinFu XinWu HuijuanChen YingLiu WenxinWang Ke