Ask about this productRelated genes to: OGG1 Blocking Peptide
- Gene:
- OGG1 NIH gene
- Name:
- 8-oxoguanine DNA glycosylase
- Previous symbol:
- -
- Synonyms:
- HMMH, HOGG1, OGH1, MUTM
- Chromosome:
- 3p25.3
- Locus Type:
- gene with protein product
- Date approved:
- 1997-07-22
- Date modifiied:
- 2016-10-05
Related products to: OGG1 Blocking Peptide
Related articles to: OGG1 Blocking Peptide
- Epstein-Barr virus (EBV) establishes lifelong latency in human cells and can periodically reactivate, contributing to several cancers. However, the molecular mechanisms that disrupt EBV latency, particularly those driven by oxidative stress, require further investigation. In this study, we provide evidence that oxidative DNA damage, particularly the formation of 8-oxoguanine and its repair enzyme OGG1, is involved in EBV reactivation. We demonstrated that OGG1 is recruited to EBV regulatory regions under oxidative stress conditions and is associated with the transcriptional activation of immediate-early and early lytic genes. Further experiments showed that pharmacological inhibition of OGG1 DNA binding significantly suppressed EBV lytic gene expression, supporting a functional role for OGG1 in viral reactivation. Mechanistically, our findings suggest that OGG1 may contribute to EBV lytic activation through a noncanonical mechanism independent of its glycosylase activity. These findings provide insight into how EBV may exploit host oxidative DNA damage responses to facilitate latency disruption and suggest that targeting OGG1 may offer a potential strategy to limit EBV reactivation and EBV-associated diseases. - Source: PubMed
Publication date: 2026/06/06
Hao WenjingWang XiaoqiLi JiaboLiu GangPan LangBa XueqingBoldogh IstvanDuan Ziyuan - DNA oxidation damage and its repair are essential for maintaining genomic integrity in the human limbal epithelium, which harbors corneal epithelial stem cells. This study investigated the distribution of the DNA base oxidation 8-oxoguanine (8-oxoG) and the base excision repair (BER) enzymes 8-oxoguanine DNA glycosylase (OGG1) and apurinic/apyrimidinic endonuclease 1 (APE1) in non-cultured and eye-bank organ-cultured human limbal epithelia. Immunohistochemistry was used to assess the localization and staining intensity of 8-oxoG, OGG1, and APE1, evaluated semi-quantitatively and by image analysis. In situ hybridization was performed to detect the distribution of and gene expression in organ-cultured tissue. In non-cultured limbal epithelia, nuclear 8-oxoG staining was more frequently observed in superficial epithelial layers, whereas nuclear OGG1 and APE1 staining predominated in basal layers. In organ-cultured epithelia, a higher proportion of superficial nuclei exhibited 8-oxoG staining, while the basal predominance of OGG1 was reduced and that of APE1 was preserved. Transcripts of and were detected in basal- as well as in suprabasal layers of organ-cultured epithelia. These findings demonstrate the presence of DNA base oxidation and BER-related enzymes in basal and suprabasal human limbal epithelial cells during storage of corneal tissue under commonly used eye-bank organ-cultured conditions prior to transplantation. - Source: PubMed
Publication date: 2026/06/04
Nicolaissen Bjørn OttoNguyen GiangBeraki KahsaiAzqueta AmayaPetrovski GoranMoe Morten CKrohn-Hansen DagCollins Andrew RNicolaissen BjørnLorenzo Yolanda - Acute myocardial infarction (AMI) is a major cause of cardiovascular death, with STEMI and NSTEMI as distinct subtypes. Current diagnostic tools often lack the sensitivity and timeliness to rapidly distinguish these subtypes at early clinical presentation. The DNA damage response (DDR), activated by ischemia and oxidative stress during AMI, represents a promising pathway for identifying novel biomarkers. Despite evidence of its role in myocardial infarction, little is known about DDR gene expression in South Asian populations, particularly in Bangladesh. This study evaluated the expression of four DDR genes; ATM, CDK7, OGG1, and NBN and their potential for distinguishing between STEMI and NSTEMI. - Source: PubMed
Ripon Rifat HossainZahin HasnatNoman Abdullah AlSaba Abdullah AlSayem MohammadNabi A H M NurunYasmin Tahirah - Systemic sclerosis (SSc) is a chronic, autoimmune, fibrotic disorder involving immune dysregulation, vascular abnormalities and progressive fibrosis. Although oxidative stress and defective DNA repair have been implicated in its pathogenesis, the impact of vitamin D on DNA repair pathways remains unclear. This study aimed to investigate the expression of DNA repair enzymes in SSc, explore their relationship with vitamin D status and assess the effects of vitamin D supplementation on the transcriptional expression of these enzymes. Peripheral blood samples were collected from 52 female patients with SSc and 31 age-matched healthy controls (HCs). Gene expression levels of base excision repair (BER) enzymes (APE1 and OGG1) and nucleotide excision repair (NER) enzymes (XPA and XPC) were analyzed. Serum vitamin D levels were measured and correlated with disease activity scores. In a prospective arm of the study, patients received six months of vitamin D supplementation and their DNA repair capacity was evaluated pre- and post-intervention. Baseline expression of APE1 and OGG1 was significantly lower in SSc patients than in HCs, whereas expression of the NER genes remained unchanged, indicating selective impairment of the BER pathway. Vitamin D deficiency was prevalent in SSc and inversely correlated with disease severity. Supplementation significantly increased serum vitamin D levels and up-regulated APE1 and OGG1 expression; while NER genes remained unaffected. These findings are consistent with evidence of elevated oxidative DNA lesions in SSc and support a mechanistic link between BER activity and the repair of oxidative DNA damage. SSc patients exhibit reduced transcription of BER-specific enzymes associated with vitamin D deficiency andrestoration of vitamin D levels partially rescues BER enzyme expression. These findingshighlight a potentially modifiable axis linking micronutrient status, genomic stability and disease activity and provide a rationale for investigating vitamin D optimization as an adjunctive strategy to enhance DNA repair and potentially attenuate inflammatory and fibrotic processes in SSc. - Source: PubMed
Publication date: 2026/06/08
Dal-Bekar Nazli EcemBirdogan AliIslekel Gul HurayBirlik Ahmet MerihAkdogan Gul - 8-Oxoguanine DNA glycosylase 1 (OGG1) is a key enzyme for maintaining genomic integrity, as it specifically recognizes and excises 8-oxoguanine (OG), a major oxidative DNA lesion, to initiate base excision repair. Dysregulation of OGG1 activity is closely associated with genomic instability, apoptosis, and tumorigenesis. However, conventional methods for detecting OGG1 activity often lack the sensitivity required for trace-level analysis, limiting their application in early diagnostics and mechanistic studies. In this study, we report an innovative assay termed OG-specific rolling circle amplification (OG-RCA), which for the first time integrates OGG1-mediated OG excision with RCA and subsequent G-triplex formation for signal readout. This novel approach establishes a unique conversion strategy that translates enzymatic activity into quantifiable amplification signals, significantly enhancing detection sensitivity and specificity. After systematic optimization of key reaction conditions, including dsOG substrate concentration, enzyme amounts, and DNA probe concentrations, the OG-RCA assay achieved an exceptionally low limit of detection (LOD) of 3 × 10 mg/mL (equivalent to ∼1.6 × 10 U/mL) for OGG1 activity, surpassing existing methods. The assay also exhibited high specificity, showing minimal cross-reactivity with other DNA repair glycosylases. Moreover, spike-recovery experiments using HeLa cell protein extracts and 293T cell lysates confirmed its robustness in complex biological samples. The OG-RCA method not only provides a powerful tool for the ultrasensitive detection of DNA base damage biomarkers but also offers a novel platform for investigating DNA damage and repair mechanisms. It holds significant promise for applications involving limited biological samples and mechanistic studies of DNA repair. - Source: PubMed
Publication date: 2026/06/01
Huang Zi-QinDong Jia-HuiZhang Run-HongZhao Ling-LiLiu YingZhou Ying-LinZhang Xin-Xiang