EIF2C4 Blocking Peptide
- Known as:
- EIF2C4 Blocking Peptide
- Catalog number:
- 33r-1952
- Product Quantity:
- USD
- Category:
- -
- Supplier:
- Fitzgerald industries international
- Gene target:
- EIF2C4 Blocking Peptide
Related genes to: EIF2C4 Blocking Peptide
- Gene:
- AGO4 NIH gene
- Name:
- argonaute RISC component 4
- Previous symbol:
- EIF2C4
- Synonyms:
- hAGO4, KIAA1567, FLJ20033
- Chromosome:
- 1p34.3
- Locus Type:
- gene with protein product
- Date approved:
- 2002-04-26
- Date modifiied:
- 2019-02-01
Related products to: EIF2C4 Blocking Peptide
Related articles to: EIF2C4 Blocking Peptide
- Seed priming with dopamine reduced fluoride-bioaccumulation, induced endogenous dopamine level, thereby orchestrating phytohormone homeostasis and biogenic amine metabolism, and modulating osmolyte and antioxidant machinery to enhance fluoride- tolerance in rice. DNA methylation plays a critical role in plant immunity, yet its regulatory mechanism in cotton Verticillium wilt (VW) resistance remains elusive. Here, we demonstrate that dynamic DNA methylation is essential for cotton defense against Verticillium dahliae. Silencing of methyltransferase and demethylase genes via VIGS both compromised VW resistance, indicating that resistance depends on a balanced methylation homeostasis. Whole-genome bisulfite sequencing revealed asymmetric methylation patterns between A and D subgenomes and significant CHH hypermethylation upon pathogen infection, particularly in euchromatic regions. Integrated methylome and transcriptome analyses showed that methylation status, especially in promoter regions, inversely correlates with gene expression. The RNA-directed DNA methylation (RdDM) pathway emerged as a central regulator, with AGO4 silencing enhancing VW resistance and affecting methylation of key defense genes, including phenylpropanoid and pectin methylesterase genes. Specifically, GhCYP71, a negative regulator of VW resistance, exhibited CHH methylation differences in its 5'UTR between resistant and susceptible varieties, and its expression was directly regulated by DNA methylation. Yeast two-hybrid identified AGO4-interacting proteins including ADH1, suggesting crosstalk between epigenetic regulation and metabolic status. Our findings establish that RdDM-mediated DNA methylation reprogramming precisely modulates defense gene expression, providing a mechanistic framework for epigenetic immunity and potential targets for breeding VW-resistant cotton. - Source: PubMed
Publication date: 2026/04/19
Zhang ZixuZhang JinWang ZhichengZhang XinyuChen WeiyiJiao MengjiaXie MeixiaYang JunZhang DongmeiWang XingfenMa ZhiyingZhang Yan - In this study, we report the synthesis of silver phosphate supported on a phase mixture of Analcime and Pitiglianoite zeolites. The hybrid materials were obtained with AgP amounts of 25% (AgP_ZLT_25), 50% (AgP_ZLT_50), 75% (AgP_ZLT_75), and 95% (AgP_ZLT_95) relative to the ZLT mass. Structural characterization by X-ray diffraction (XRD) and structural refinement by the Rietveld method revealed that ZLT is composed of 71.02 ± 0.54% Analcime and 28.98 ± 0.47% Pitiglianoite zeolite. The percentage of AgP in the mixture, also quantified by the Rietveld method, resulted in values of 7.99 ± 0.46, 36.70 ± 4.84, 67.50 ± 5.06, and 93.74 ± 3.15% for the samples AgP_ZLT_25, AgP_ZLT_50, AgP_ZLT_75, and AgP_ZLT_95, respectively. Vibrational characterization by Raman and infrared (IR) spectroscopy revealed the presence of the main active vibrational modes of silver phosphate, silicate, and aluminate groups, in both pure and hybrid materials. The values revealed that ZLT effectively absorbs in the ultraviolet region, with equal to 3.65 eV. While the hybrid materials exhibited a decrease in the value, specifically between 3.63 eV (AgP_ZLT_25) and 2.35 eV (AgP_ZLT_95), in consequence of the contributions of the electronic transitions of AgP ( = 2.35 eV). The photocatalytic performance of the materials prepared in the photodegradation of RhB dye in an aqueous medium under exposure to LED-simulated visible light resulted in dye discoloration percentages of 97.07% for the AgP_ZLT_95 sample and 93.56% for the AgP_ZLT_75 sample. Furthermore, analysis of the rate constant ( ) and half-life of the reactions ( ) revealed that the AgP_ZLT_95 catalyst was approximately 1,391.5 times more efficient compared to photolysis, exhibiting superior activity in the generation of superoxide radicals and vacancies. Additionally, it was found that zeolite acts as a sacrificial material, reducing the photocorrosion process of silver phosphate. Hybrid materials, as well as AgP, exhibited high antimicrobial activity, resulting in a minimum inhibitory concentration (MIC) of 0.01562 mg mL for the bacteria and , while for the fungi and , the MIC was 0.03125 mg mL, as a result of redox processes involving reactive oxygen species (ROS), as well as silver-ion activity and [AgO] and [PO] clusters in the cell wall and internal structures of microorganisms. - Source: PubMed
Publication date: 2026/02/27
Dos Santos Leite Ygor GeannNobre Francisco Xavierde Lima Nascimento José FábioDe Sombra Quércia Wesley VictorGurgel Raiana SilveiraAlbuquerque Patrícia MelchionnaLeyet Ruiz YurimilerSargentini-Júnior ÉzioBolson Marcos ARomaguera-Barcelay YonnyPeña-Garcia Ramón Rda Costa Couceiro Paulo RogérioCarvalho Rosany Picolotto - genomes are expressed as a single large open reading frame, which is translated into a polyprotein that is post-translationally cleaved by three virus-encoded proteases into 10 functional proteins. Several of these potyviral proteins, including nuclear inclusion protein b (NIb), are multifunctional. Here, using the classic GFP silencing in gfp-transgenic plants, we show that potato virus Y (PVY) NIb, in addition to its canonical role as the viral RNA-dependent RNA polymerase (RdRP), functions as a suppressor of RNA silencing. Mutational analyses reveal a previously unreported NIb nuclear localization signal (NLS) consisting of a triple-lysine motif. NIb suppression of RNA silencing activity was lost when the NLS was mutated, suggesting that nuclear localization is required for NIb suppression of RNA silencing activity. Analysis of sequenced GFP siRNAs revealed three reproducible hotspot regions at ≈175 nt, ≈320-330 nt, and a broader 3'-proximal region spanning ≈560-700 nt that contains multiple local maxima. These data show differences in the positional distribution of siRNAs between samples expressing NIb and those expressing NIb, the NIb null mutant that does not suppress RNA silencing. However, the positional distribution of GFP-derived small RNAs across the transgene differed modestly between NIb and NIb, while both treatments showed the same three reproducible hotspot regions. Furthermore, NIb was found to interact with four key RNA silencing pathway proteins-AGO4, HSP70, HSP90, and SGS3. Except for HSP90, each of these proteins showed degradation products that were absent in NIb mutants that did not suppress RNA silencing. These findings support a role for NIb in countering host defense during virus infection. - Source: PubMed
Publication date: 2026/01/25
Niraula Prakash MHowell SaniyaaStratton Chase AMoore Michael TDopler Matthew BAbeer Muhammad IGitcho Michael AFondong Vincent N - Pancreatic adenocarcinoma (PAAD) is an aggressive cancer type with high mortality rates. The Argonaute (AGO) gene/protein family is an evolutionary conserved family, which is responsible for post-transcriptional regulation of gene expression. Despite the fact that this family members (AGO1-4) have been linked to prognosis in some cancers, they have not been comprehensively investigated in PAAD. Therefore, this study investigates the role of AGO family members on PAAD. - Source: PubMed
Gulbey OzcanDuran Tugce - ARGONAUTE4 (AGO4)-mediated RNA-directed DNA methylation (RdDM) defends against DNA viruses by methylating their genomes. However, there is limited information available regarding RNA viruses. Here, we show that OsAGO4 has antiviral immunity against two different types of RNA viruses, rice stripe virus (RSV, Tenuivirus) and Southern rice black streaked dwarf virus (SRBSDV, Fijivirus). To facilitate infection, the evolutionarily distinct viral effectors RSV P2 and SRBSDV SP8 both targeted OsAGO4 for degradation. These unrelated viral proteins both recruited the F-box protein OsFBX68 to promote their association with OsAGO4, resulting in enhanced OsAGO4 degradation. In summary, our findings elucidated OsAGO4-mediated antiviral defense and reveal new mechanisms by which diverse RNA viral effectors exploit OsAGO4 to promote infection using a common counter-defense strategy. - Source: PubMed
Xie KailiXu ZhongtianQi QinglingLi YanjunHu XiaodiYan WenkaiZhang HehongLi LuluChen JianpingSun Zongtao