Ask about this productRelated genes to: PPID Blocking Peptide
- Gene:
- PPID NIH gene
- Name:
- peptidylprolyl isomerase D
- Previous symbol:
- -
- Synonyms:
- CYP-40
- Chromosome:
- 4q32.1
- Locus Type:
- gene with protein product
- Date approved:
- 1995-08-23
- Date modifiied:
- 2016-10-05
Related products to: PPID Blocking Peptide
Related articles to: PPID Blocking Peptide
- Diagnosis of PPID in horses relies on measurement of plasma adrenocorticotrophic hormone (ACTH) concentrations. Because measured ACTH values vary between analytical platforms, and published diagnostic thresholds apply almost exclusively to the Immulite 1000 chemiluminescent assay (CLA), there is a need for validated, assay-specific thresholds for contemporary analysers. This study aimed to transfer and validate established Immulite 1000 diagnostic thresholds for plasma ACTH to three contemporary systems (Immulite 2000XPi, Tosoh AIA-900, and Tosoh AIA-360) using the Clinical Laboratory Standards Institute (CLSI) guidelines. Surplus clinical EDTA plasma samples were analysed across pairs of analysers to enable method comparison and reference interval transference. The Immulite 2000XPi showed close agreement with the Immulite 1000 (r=0.962, P<0.0001; bias -3.9 pg/mL (95% CI: -12.2-4.4)), enabling reliable linear transference of thresholds. Comparison of Immulite 2000XPi with the AIA-900 revealed moderate correlation and non-linear, concentration-dependent bias (r=0.770, P<0.0001; bias 30.8 pg/mL (95% CI: -110-171)), necessitating threshold-specific regression. The AIA-360 and AIA-900 showed excellent agreement (r=0.993, P<0.0001; bias 4.023 pg/mL (95% CI: -1.4-9.4)). Platform-specific low (90% sensitivity) and high (90% specificity) diagnostic thresholds were generated for four seasonal periods for all analysers. The proposed analyser-specific thresholds provide clinicians and laboratories with more validated guidance for interpreting plasma ACTH results across contemporary analysers, supporting more accurate diagnosis and management of PPID in horses. - Source: PubMed
Publication date: 2026/04/23
Durham Andy ELopes Ana - Uropathogenic (UPEC) is the primary etiological agent of urinary tract infections (UTIs) worldwide. The emergence of strains combining high virulence with multidrug resistance (MDR) poses a significant challenge to public health. This study aimed to characterize the phylogenetic distribution, virulence profiles, and antimicrobial susceptibility of UPEC isolates recovered from patients in the metropolitan area of Buenos Aires (AMBA), Argentina. Phylogenetic groups, the ST131 lineage, and virulence-associated genes were identified using PCR-based assays. Antimicrobial susceptibility testing (AST) was performed using automated methods and extended-spectrum beta-lactamase (ESBL) production was confirmed using the double-disk synergy test. Colistin (COL) resistance was evaluated by Colistin Drop Test and PCR screening for the (mobile colistin resistance gene 1). Biofilm formation was detected by the Tissue Culture Plate (TCP) method, whereas phenotypic virulence factors (VF) were assessed with Congo Red agar, hemagglutination, and hemolysis assays. Phylogenetic groups B2 (43.8%) and D (26.7%), typically associated with extraintestinal infections, were the most frequent. The high-risk clone B2-ST131 was detected in 6.7% of isolates. Biofilm production was observed in 92.4% of the isolates, with curli fimbriae (87.6%) being the most frequently expressed VF. The highest resistance rates were observed for ampicillin (62.1%), ampicillin-sulbactam (39.8%), and trimethoprim-sulfamethoxazole (25.2%). Interestingly, 3.8% of isolates exhibited colistin resistance, despite the absence of the gene. This study highlights the detection of MDR-UPEC isolates that showed strong resistance to fluoroquinolones and were ESBL producers with high virulence in Argentina, justifying future research encompassing genomic and epidemiological monitoring of local UPEC, which is essential for managing infections and developing new therapeutic and preventive measures. - Source: PubMed
Publication date: 2026/03/29
Molina Nora BGonzález Pasayo Ramón ALópez Marisa ASparo Mónica D - Host factors that promote or restrict human immunodeficiency virus (HIV) infection in human CD4+ T cells have not been comprehensively identified. We employed orthogonal genome-wide CRISPR activation (CRISPRa) and CRISPR knockout screens in primary CD4+ T cells to discover pro- and anti-HIV host factors systematically. Secondary pooled screens and individual perturbations validated high-confidence hits and revealed diverse mechanisms of action. CRISPRa uncovered multiple potent antiviral factors, including PI16, PPID, SHISA3, and ITM2A. PI16 interacts with host factors involved in HIV fusion and inhibits viral entry, whereas PPID (Cyp40), a paralog of the proviral cyclophilin CypA, binds capsid and reduces nuclear import of the HIV core. Structural modeling, evolutionary analyses, and targeted mutagenesis revealed domains and residues required for PPID-mediated HIV restriction, including non-human primate ortholog substitutions that enhance antiviral activity. Together, these data define the functional HIV-host interaction landscape in primary human T cells and uncover new mechanisms modulating infection. - Source: PubMed
Publication date: 2026/04/20
Rathore UjjwalDugan EliThornton HunterKumar Vigneshwari EaswarDajani RamaBurdick Ryan CYoung Janet MSteinhart ZacharyLao ReannaDelviks-Frankenberry Krista AChoi WooyoungHenriques William SEcheverria IgnaciaDann EmmaDureja IshaanPathak NandiniArce Maya MMcKetney JustinUmhoefer Jennifer MParulekar SimrunSchmidt RalfPolacco Benjamin JNeidleman JasonMontano MauricioNguyen Vinh QSali AndrejLevy Jay ATenthorey Jeannette LCheng YifanRoan Nadia RSwaney Danielle LKaake Robyn MDodgson Stacie EHiatt JosephPathak Vinay KMalik Harmit SKrogan Nevan JMarson Alexander - Insulin dysregulation (ID) is common and diminishes welfare of horses. Current management relies on diet and exercise, with variable responses and limited medical options. Alpha-glucosidase inhibitors (AGIs) might provide adjunctive therapy. - Source: PubMed
Whitfield-Cargile CanaanColeman MichelleHart KelseyGomes DanielBerghaus LondaDuberstein Kylee JoEllis KenzieTinkle AmandaShirzad Roya - Pituitary pars intermedia dysfunction (PPID) is an age-related endocrinopathy associated with elevated systemic inflammation, and specifically an upregulation of interleukin-8 (IL-8). It is unknown if PPID in concomitant with reproductive tract inflammation. This is a pertinent question, as chronic inflammation of the endometrium and ovary would impede fertility. Therefore, the objective of this study was to evaluate the impact of PPID on the reproductive tract of the mare. - Source: PubMed
Publication date: 2026/03/26
Howard JocelynHamner IsabellaCrook Rebecca AElliott CheyenneCarnevale ElaineColeman Stephen JKlinglesmith Brody AMcCue Patrick MSones Jenny LFedorka Carleigh E