Ask about this productRelated genes to: MYBPH Blocking Peptide
- Gene:
- MYBPH NIH gene
- Name:
- myosin binding protein H
- Previous symbol:
- -
- Synonyms:
- -
- Chromosome:
- 1q32.1
- Locus Type:
- gene with protein product
- Date approved:
- 1993-06-18
- Date modifiied:
- 2015-09-04
Related products to: MYBPH Blocking Peptide
Related articles to: MYBPH Blocking Peptide
- Intramuscular fat (IMF) content is a critical determinant of meat quality, directly influencing flavor and palatability. RNA editing, a prevalent posttranscriptional modification in mammals, has been extensively investigated in cattle; however, its role in bovine intramuscular fat deposition remains poorly understood. Here, we performed a comprehensive analysis of RNA editing differences in the longissimus dorsi muscle between cattle groups with divergent IMF content to identify editing sites potentially associated with IMF deposition. A total of 283 581 RNA editing sites (RESs) were identified from transcriptomic data of cattle with contrasting IMF phenotypes. Among the differential A-to-I editing sites located in coding sequences, 14 led to amino acid alterations, including an editing event in MYBPH, a gene with important functions in muscle and adipose tissues. Differential A-to-I editing sites in the 3' UTR resulted in altered miRNA binding patterns: 30 sites led to loss of miRNA binding, whereas 16 sites gained novel miRNA binding capacity. Dual-luciferase reporter assays confirmed that the editing site chr2:106787199 in the 3' UTR of PRKAG3 affects binding by bta-miR-23b-5p. Functional validation experiments demonstrated that bta-miR-23b-5p promotes PRKAG3 expression, and overexpression of PRKAG3 suppresses preadipocyte differentiation and lipid droplet formation. Collectively, these findings uncover a regulatory role for RESs in IMF deposition and provide new insights into the functional significance of RNA editing in bovine intramuscular fat accumulation. - Source: PubMed
Li ChenglongZhou XiaonanDing YanlingXu JunjieLiang JiahaoHou Li'eKang Xiaolong - The regulatory mechanisms underlying the interaction between fatty acid (FA) profiles and gene expression are highly complex, involving signaling pathways and transcription factors that control lipid metabolism. Using the gene co-expression approach, we can identify key gene regulators and gain a better understanding of gene interactions that may play an important role in regulatory mechanisms. Therefore, this study aims to identify gene-expression regulatory mechanisms associated with FA deposition profiles in skeletal muscle across different diets. We used basal diets with different levels of soybean oil (1.5% soybean oil [SOY1.5], reference diet; or 3% soybean oil [SOY3.0], enriched diet) added during the growth and finishing phases in a 98-day study. Total RNA was extracted, and mRNA was sequenced (Illumina). Bioinformatics analysis was performed with quality control, preprocessing, and alignment using Sus scrofa11.1. Gene abundance was normalized to transcripts per million. To identify co-expressed modules, we used weighted gene co-expression network analysis (WGCNA) with RNA-Seq data and the deposited FA profile. After filtering, data from 33 immunocastrated male pigs were used in this study. To identify pathways and Gene Ontology (GO) terms affected by the enriched diet (with 3% soybean oil), DAVID and REVIGO were used. Diets with varying levels of soybean oil affect metabolic processes differently. In general, we identified co-expression networks mainly involved in lipid metabolism, diseases and general response involved in inflammatory processes, glucose homeostasis. We constructed co-expression networks and identified the hub genes, including TPM1 and SLC38A10, as well as CSRNP1, TRIP10, ZFP30, and MYBPH. Co-expression analysis using WGCNA provides new insights into fatty acid deposition by identifying candidate genes potentially involved in lipid regulation and influenced by dietary differences. - Source: PubMed
Publication date: 2026/06/11
Fanalli Simara LarissaCrooijmans Richard P M AGervásio Izally CarvalhoGomes Julia Dezende Almeida Vivian VezzoniMoreira Gabriel Costa Monteirode Alencar Severino MatiasCesar Aline Silva Mello - Dermatomyositis is a common immune-mediated skin disorder whose pathogenesis has not been fully elucidated. Environmental factors play a key role in its onset and progression. Bisphenol A (BPA) is a widespread environmental pollutant known to pose risks to human health. Previous studies have indicated that BPA exposure can disrupt immune function and trigger skin inflammation and autoimmune diseases. However, the role and molecular mechanisms of BPA in dermatomyositis remain unclear. This study aims to systematically elucidate whether and how bisphenol A (BPA) may contribute to the development of dermatomyositis by identifying key toxicological targets and underlying molecular mechanisms through an integrated computational framework. - Source: PubMed
Publication date: 2026/03/30
Cheng LiliTang ZhongfuLi MingHuang Chuanbing - A detailed analysis of how muscle fiber nuclei (myonuclei) respond to a hypertrophic stimulus could provide a critical step toward understanding compromised skeletal muscle plasticity with age. We used recombination-independent doxycycline-inducible myonucleus-specific fluorescent labelling, tissue RNA-sequencing, myonuclear DNA methylation analysis, multi-omic integration, and single myonucleus RNA-sequencing (smnRNA-seq) to define the molecular characteristics of adult (6-8 month) and aged (24 month) murine skeletal muscle after acute mechanical overload (MOV). In adult and aged MOV muscles, we found that: 1) similarities in the transcriptional response to loading-specifically in metabolism genes - were partly explained by a post-transcriptional microRNA-mediated mechanism that we corroborated using an inducible muscle fiber-specific miR-1 knockout model, 2) differences in age-dependent transcriptional responses were linked to the magnitude and location of differential DNA methylation in resident myonuclei, specifically around genes such as Myc, Runx1, Mybph, Ankrd1, collagen (Col) genes, and minichromosome maintenance (Mcm) genes, 3) adult and aged resident myonuclear transcriptomes had differing enrichment for innervation-related transcripts as well as unique transcriptional profiles in an Atf3+ "sarcomere assembly" population after MOV, and 4) cellular deconvolution analysis and smnRNA-seq supports a role for neuromuscular junction regulation in age-specific hypertrophic adaptation. These data are a roadmap for uncovering molecular targets to enhance aged muscle adaptability. - Source: PubMed
Publication date: 2026/02/17
Koopmans Pieter JJones Ronald GCabrera Ana ReginaMorena FranciellyGreene Nicholas PMcCarthy John JIsmaeel AhmedWen YuanMurach Kevin A - A detailed analysis of how muscle fiber nuclei (myonuclei) respond to a hypertrophic stimulus would provide a critical step toward understanding compromised skeletal muscle plasticity with age. We used recombination-independent doxycycline-inducible myonucleus-specific fluorescent labelling, tissue RNA-sequencing, myonuclear DNA methylation analysis, multi-omic integration, and single myonucleus RNA-sequencing to define the molecular characteristics of adult (6-8 month) and aged (24 month) murine skeletal muscle after acute mechanical overload (MOV). In adult and aged MOV muscles, we found that: 1) similarities in the transcriptional response to loading - specifically in metabolism genes - were partly explained by a post-transcriptional microRNA-mediated mechanism, which we corroborated using an inducible muscle fiber-specific knockout model, 2) differences in age-dependent transcriptional responses were linked to the magnitude and location of differential DNA methylation in resident myonuclei, specifically around hypertrophy-associated genes such as , , , collagen genes, and minichromosome maintenance genes, 3) adult and aged resident myonuclear transcriptomes had differing enrichment for innervation-related transcripts as well as unique transcriptional profiles in an + "sarcomere assembly" population after MOV, and 4) cellular deconvolution analysis supports a role for neuromuscular junction regulation in age-specific hypertrophic adaptation. These data are a roadmap for uncovering molecular targets to enhance aged muscle adaptability. - Source: PubMed
Publication date: 2025/10/30
Koopmans Pieter JJones Ronald GCabrera Ana ReginaMorena FranciellyGreene Nicholas PMcCarthy John JIsmaeel AhmedWen YuanMurach Kevin A