Ask about this productRelated genes to: NPFFR2 antibody
- Gene:
- NPFFR2 NIH gene
- Name:
- neuropeptide FF receptor 2
- Previous symbol:
- GPR74
- Synonyms:
- NPFF2, NPGPR
- Chromosome:
- 4q13.3
- Locus Type:
- gene with protein product
- Date approved:
- 1999-07-30
- Date modifiied:
- 2016-10-05
Related products to: NPFFR2 antibody
Related articles to: NPFFR2 antibody
- Neuropeptide FF receptor 2 (NPFFR2) plays a role in bioelectric field regulation in bone marrow-derived macrophages (BMDMs), but the influence of electric fields on NPFFR2 protein behavior remains unclear. This study investigates the effects of direct current electric field (dcEF) stimulation on NPFFR2 through cellular experiments and molecular dynamics (MD) simulations. The findings revealed that after applying physiologically relevant dcEF intensities (50 and 200 mV/mm) to wild-type and Npffr2-knockout BMDMs, cell viability was not significantly affected, but notable morphological changes occurred in both cell types. The dcEF stimulation suppressed NPFFR2 protein expression and induced cytoskeletal rearrangement in wild-type BMDMs. Furthermore, transcriptomic analysis revealed extensive gene expression changes, while MD simulations demonstrated that NPFFR2 conformation is sensitive to electric field strength in an intensity-dependent manner. Overall, these findings provide new insights into the interplay between a physical stimulus and a key immune receptor, suggesting that dcEF may regulate macrophage function by altering NPFFR2 protein levels and conformational stability. - Source: PubMed
Publication date: 2025/12/03
Zuo ZhuoWang YaxingZhao MengyaFang YanweiChen YunfeiZuo ZiyiSun Yulong - Prolactin-releasing peptide and its cognate receptor, G protein-coupled receptor (GPR)10, are important in the physiological regulation of body weight in both rodents and humans. Here, we describe a modified peptide, NN501, with agonist properties at both GPR10 and neuropeptide FF receptor 2 (NPFFR2), which reduces body weight when administered systemically without causing obvious aversive responses. Weight reduction is similar to that of glucagon-like peptide 1 (GLP-1) receptor agonists, but with only a modest effect on food intake, suggesting a different weight-lowering mechanism. Moreover, when treatment is discontinued, mice receiving NN501 display a more gradual weight regain and no compensatory hyperphagic response (as is observed with caloric restriction and GLP-1 receptor agonism). Instead, NN501 increases energy expenditure on treatment and has a sustained effect on fatty-acid oxidation. These results indicate that GPR10/NPFFR2 agonism produces weight loss by alternative mechanisms to GLP-1 receptor agonism, suggesting it could be a viable alternative or complementary therapy for obesity. - Source: PubMed
Publication date: 2025/12/01
Feetham Claire HGroom SamJohn Linu MChristoffersen Berit OstergaardCollabolletta ValeriaLyons DavidAdamson AntonyLundh SofiaGerstenberg Marina KjærgaardTang-Christensen MadsConde-Frieboes Kilian WSecher AnnaKruse Hansen Ann MariaLuckman Simon M - We evaluated four candidate SNPs ( rs137147462, rs109452259, rs134817801, and p.K232A) previously reported in relation to mastitis or milk production traits, using 10,729 test-day phenotypic records collected over 10 years from 269 Japanese Holstein cows () enrolled in the national Dairy Herd Improvement (DHI) program. Linear mixed models were used to estimate genotypic effects on somatic cell score (SCS) and to test multiple inheritance models. To assess clinical relevance, mastitis severity was further analyzed using categories defined by somatic cell counts (SCC). Among the SNPs tested, rs137147462 showed the clearest and most consistent association with SCS under a recessive model, with GG cows exhibiting higher SCS throughout lactation. Ordinal logistic regression confirmed a higher probability of progression to severe mastitis in GG cows. p.K232A showed additive effects, with the A allele increasing milk yield while lowering fat and protein percentages. AA cows also showed higher SCS under a modest recessive effect. rs134817801 and rs109452259 had minimal effects. These findings support rs137147462 as a promising marker for mastitis resistance and indicate the importance of considering not only additive but also recessive genetic models in genomic selection strategies. - Source: PubMed
Publication date: 2025/11/08
Akiyama YoshiyukiAndo TakaakiNozaki NobuhiroArif MohammadIde YutaroWang ShaohsuMiura Naoki - Neuropeptide FF receptor 2 (NPFFR2) is a key regulator of energy homeostasis, influencing feeding behavior, insulin sensitivity, and lipid metabolism. This study investigates the metabolic consequences of Npffr2 deletion in a mouse model of diet-induced obesity. Wild-type and Npffr2 knockout mice were fed a high-fat, high-sucrose diet to induce obesity, followed by comprehensive metabolic assessments. Npffr2 knockout mice exhibited reduced food intake, accompanied by significant downregulation of hypothalamic orexigenic neuropeptides agouti-related peptide and neuropeptide Y. Enhanced energy expenditure was observed in knockout mice, as evidenced by increased thermogenic capacity, elevated uncoupling protein 1 expression in brown adipose tissue, and improved core temperature maintenance under cold exposure. Lipid metabolism was also improved, with reduced hepatic and adipose lipid accumulation and lower circulating triglyceride and non-esterified fatty acid levels. Molecular analyses revealed increased AKT phosphorylation in the hypothalamus and skeletal muscle, along with downregulation of protein tyrosine phosphatase 1B in the mediobasal hypothalamus, indicating improved central and peripheral insulin signaling. Here, we demonstrated that NPFFR2 plays a critical role in obesity-associated energy regulation, lipid accumulation, and insulin resistance. These findings highlight NPFFR2 as a potential therapeutic target for obesity and related metabolic disorders. - Source: PubMed
Hsu Hsiang-TingHsu Chun-ChunTsai Sze-ChiChen Jin-ChungLi Hui-YunLin Ya-Tin - Red Sindhi cattle, a distinguished dairy breed from India, are famous for their resilience in tropical climates and exceptional milk yield. This study utilized double digest restriction site-associated DNA sequencing (ddRADseq) across 96 individuals to explore genome-wide diversity and uncover signatures of selection. The analysis revealed a high proportion of polymorphic SNPs (0.956), moderate nucleotide diversity (π = 0.215 ± 0.114), and a low minor allele frequency (MAF = 0.149 ± 0.128). The analysis of Red Sindhi data showed a steep decline in effective population size (Ne) from 2387 to 125.9 over 13 generations, implying potential bottlenecks and underscoring the urgency of conservation efforts. Employing Tajima's D, composite likelihood ratio (CLR), integrated haplotype score (iHS), and runs of homozygosity (ROH) methods, we identified 490 genomic regions under positive selection, encompassing 1282 genes and aligning with 574 quantitative trait loci (QTLs). Functional annotations highlighted several genes linked to reproduction (RHOU, MND1), production (DOK6, NPFFR2), immune response (BOLA-DYA and BOLA-DMB), and environmental adaptation (HSPA14, NOD2, GCLC, and RPS19BP1). Several MHC class II genes under selection pressure indicate robust immune competence, while stress-response genes supported Red Sindhi's remarkable tolerance to extreme heat. These findings show the breed's strong adaptability and disease resilience, underlining its importance as a valuable genetic resource for improving livestock in challenging environments. - Source: PubMed
Publication date: 2025/10/10
Nayak Sonali SonejitaPanigrahi ManjitRajawat DivyaSahoo Sarada PrasannaDutt Triveni