Ask about this productRelated genes to: CASP6 antibody
- Gene:
- CASP6 NIH gene
- Name:
- caspase 6
- Previous symbol:
- -
- Synonyms:
- MCH2
- Chromosome:
- 4q25
- Locus Type:
- gene with protein product
- Date approved:
- 1996-07-22
- Date modifiied:
- 2015-11-12
Related products to: CASP6 antibody
Related articles to: CASP6 antibody
- Caspases are cysteine proteases that regulate programmed cell death. While caspase-6, an executioner caspase, is known for its role in neurodegeneration and cell death, its broader physiological functions remain poorly understood. Our previous study revealed that caspase-6 drives liver injury and fibrosis in metabolic dysfunction-associate steatohepatitis. Here, we report that caspase-6 deficiency protects against high fat diet-induced obesity. Both global and adipocyte-specific caspase-6 knockout mice exhibit increased energy expenditure, reduced adiposity and inflammation, and improved glucose metabolism. Mechanistically, caspase-6 directly cleaves peroxisome proliferator-activated receptor gamma (PPARγ) and its cofactor specificity protein 1 (SP1), thereby suppressing adipose triglyceride lipase (ATGL) expression. Caspase-6 deficiency restores ATGL, enhancing lipolysis and elevating fatty acyl esters of hydroxy fatty acids (FAHFAs), which alleviate inflammation and enhance insulin sensitivity. These findings uncover a novel Casp6-PPARγ/SP1-ATGL axis in adipose tissue and establish caspase-6 as a potential therapeutic target for obesity and insulin resistance. - Source: PubMed
Gupta AbhishekYou WenjingHan LinmengJi JianfeiPan MeixiaHan XianlinSun XiaoliZhao Peng - Osteosarcoma, the most common primary malignant bone tumour, presents significant treatment challenges due to its complex tumour microenvironment and the development of chemoresistance. This study employs single-cell transcriptomics to investigate chemotherapy-induced changes in osteosarcoma at both the cellular and molecular levels. Single-cell RNA sequencing data were analysed to identify cell subpopulations and their responses to chemotherapy. Differential gene expression and pathway enrichment analyses were performed to elucidate chemotherapy-induced changes. Additionally, we developed and validated a predictive model based on pyroptosis-related genes, named Pyroscore, using 101 different machine-learning algorithms. Chemotherapy led to an increased proportion of osteoclasts, endothelial cells, mesenchymal stem cells and pericytes, while decreasing T and NK cells, B cells, chondroblasts, monocytes and macrophages. Chemotherapy markedly upregulates the pyroptosis pathway in tumour cells, suggesting that chemotherapy induces programmed cell death in cancer cells through the activation of pyroptosis. Metabolic pathway analysis revealed significant inhibition of sulphur metabolism, starch and sucrose metabolism, pentose phosphate pathway, inositol phosphate metabolism, nitrogen metabolism and fatty acid metabolism. The Pyroscore model, which incorporates BAK1, CASP1, CASP5 and CASP6, demonstrated robust prognostic value across multiple data sets, with high scores correlating with improved survival outcomes. This study highlights the impact of chemotherapy on osteosarcoma cell subpopulations and the tumour microenvironment. The activation of the pyroptosis pathway and the development of the pyroscore prognostic model provide new insights into the mechanisms of chemotherapy response and potential therapeutic targets. These findings underscore the importance of personalized treatment strategies in improving outcomes for osteosarcoma patients. - Source: PubMed
Jin TaoDong LeiKai WangYu ZiyangYu GuoyongLiu Weifeng - Caspases are pivotal executioners of apoptosis and inflammation, playing critical roles in vertebrate immune defense. However, their evolutionary dynamics and functional characteristics in basal ray-finned fish, such as sturgeons, remain largely unexplored. In this study, we performed a genome-wide identification and characterization of the caspase (CASP) gene family in three sturgeons (Acipenser ruthenus, Polyodon spathula, and Acipenser sinensis). Phylogenetic and synteny analyses revealed a lineage-specific expansion of the sturgeon CASPs repertoire, particularly within the CASP8 and CASP3/7 subfamilies. Despite the variation in gene copy number, the core protein domains and motifs remained highly conserved. Expression profiling demonstrated that specific CASPs members exhibit distinct spatial distribution patterns in mucosal immune tissues and validated by qRT-PCR. Notably, inflammatory CASP1 and initiator CASP9 were enriched in the gill and duodenum, whereas effector CASP3 and CASP6 showed preferential expression in the valvular intestine. Furthermore, re-analysis of public transcriptomic datasets revealed that in vivo bacterial infections triggered a robust and systemic upregulation of the caspase network, contrasting with the moderate responses observed in in vitro cell models. Collectively, these findings highlight the evolutionary plasticity of the caspase family in acipenseriforms and underscore their specialized roles in maintaining mucosal homeostasis and combating bacterial pathogens. - Source: PubMed
Publication date: 2026/03/06
Zhu HaoGeng YiLi ZhiqiongChen Defang - A novel synthetic bromophenol (BP), inspired by marine-derived natural bromophenols, was evaluated for its antitumor activity and for the enhancement of its in vitro performance through liposomal encapsulation (LipoBP). Etoposide was used as a reference in characterization, release, and loading studies. PEGylated liposomes were employed to improve BP's solubility, bioavailability, and therapeutic potential. The cytotoxicity, apoptosis, and gene expression effects of free BP and LipoBP were assessed in A549 (lung) and MCF-7 (breast) cancer cell lines. WST-8 assays showed that encapsulation significantly increased BP's cytotoxic activity, particularly in A549 cells, while flow cytometry and Annexin V-FITC/PI analyses indicated more pronounced apoptotic induction by LipoBP compared with free BP. qRT-PCR analyses revealed upregulation of proapoptotic genes (BAX, CASP6, CASP3 and CASP9) and downregulation of antiapoptotic/survival genes (BCL-XL, IQSEC2) in both cell lines, indicating activation of intrinsic apoptotic pathways. Plain liposomes exhibited minimal cytotoxicity, confirming their biocompatibility. Liposomal bromophenol, which we have introduced to the literature for the first time, is expected to be a promising nanocarrier system that could be effective in cancer treatment by improving the therapeutic index of new drug candidates such as marine bromophenols. - Source: PubMed
Publication date: 2026/02/13
Oztanrikulu Bercem DilanOzdemir EkremAvci BahriGöksu SüleymanBayrakceken Handan UguzAskin Hakan - The poultry industry faces severe heat-stress challenges that threaten both economic sustainability and animal welfare. Embryonic thermal manipulation (ETM) has been proposed as a thermal programming strategy to enhance chick heat tolerance, yet its efficacy in layers requires verification, and its effects on growth performance and neurodevelopment remain unclear. - Source: PubMed
Publication date: 2025/12/30
Fan ZixuanJie YuchenNiu BowenWu XinyuChen XingyingLi JunyingShao Li-Wa