Ask about this productRelated genes to: CASP6 antibody
- Gene:
- CASP6 NIH gene
- Name:
- caspase 6
- Previous symbol:
- -
- Synonyms:
- MCH2
- Chromosome:
- 4q25
- Locus Type:
- gene with protein product
- Date approved:
- 1996-07-22
- Date modifiied:
- 2015-11-12
Related products to: CASP6 antibody
Related articles to: CASP6 antibody
- Invertebrates rely exclusively on innate immunity but exhibit memory-like responses termed immune priming or trained immunity. In the commercially vital whiteleg shrimp (), infection by causes severe economic losses, yet the molecular networks driving secondary immune recall remain poorly understood. In this study, we established a two-step immune challenge model in using formaldehyde-inactivated and performed transcriptomic analysis on hemocytes to compare primary and secondary immune responses. Differentially expressed gene (DEG) screening and enrichment analyses (GO, KEGG, and GSEA) suggest that shrimp hemocytes undergo a broad and coordinated transcriptional reprogramming rather than uniform upregulation of immune genes. Transcriptomic data show potential associations between secondary immune priming and the modulation of cell fate processes: genes related to cell cycle progression (e.g., CDK1, CCNB3) and spindle assembly (e.g., MPS1) were significantly upregulated alongside apoptosis inhibition (CASP6 downregulation). Concurrently, metabolic remodeling was observed through the upregulation of lipid synthesis (SREBF1, FASN) and carbohydrate uptake pathways, potentially providing anabolic support for hemocyte growth and immune activation. Furthermore, the humoral effector responses appear to be strengthened, characterized by upregulated antimicrobial peptides (PEN, ALF) and the proPO melanization cascade (PPAF3, PPO3), whereas the expression of intracellular NLR was relatively suppressed, which might help mitigate excessive immune inflammation and immunopathological damage. Collectively, these transcriptomic findings identify a putative coordinated transcriptional signature of hemocyte recall responses in . This study expands our understanding of innate immune memory in invertebrates and provides candidate molecular markers for further study in disease-resistant breeding research in shrimp aquaculture. - Source: PubMed
Publication date: 2026/06/18
Li ZhongyingLi ShihaoLv XinjiaLi Fuhua - Caspase-6 (casp-6), is a member of the apoptotic family of caspases (cysteine aspartic proteases) and has been shown to be involved in several neurodegenerative diseases, including Alzheimer's disease. Drug discovery efforts have focused on specific inhibition of casp-6, and it is important that lead compounds demonstrate engagement and inhibition of the protease in vivo. A casp-6 overexpressing mouse model was reported, but is no longer available to the scientific community. We developed alternative means of activating casp-6 in commercially available mice as a platform to test potential casp-6 inhibitors. Lipopolysaccharides (LPS) from E. coli was used to stimulate an immunogenic response in WT C57BL/6NJ mice, which has been shown to activate the NLRP1 inflammasome, which activates casp-1 and subsequently, casp-6. Key tissues (brain, colon, and thymus) were sampled and evaluated for casp-6 activation by immunoblotting for both active casp-6 and a cleaved lamin A, a casp-6 substrate, and by directly measuring VEIDase activity in tissue lysates. Using in-house bred WT C57BL/6NJ mice, which had never been subjected to shipping, with a single intraperitoneal dose of LPS (5 mg/kg) and a 4-hour incubation, we observed robust casp-6 activation in the thymus. We also established VEIDase activity assays as a more reliable marker of casp-6 function than monitoring levels of casp-6 or cleaved substrates, and established the importance of using a non-immunogenic vehicle. We observed casp-6 activation in aged mice as a second useful model, which also further implicates casp-6 in age-related neurodegenerative pathways. These are new means to test the efficacy of casp-6 inhibitors using a simple in vivo assay that can robustly activate casp-6 in an easily accessible laboratory mouse strain. We foresee that these developments will drive drug discovery efforts aimed at identifying casp-6 inhibitors in various disease contexts, including neurodegeneration. - Source: PubMed
Publication date: 2026/06/17
Sagarbarria IrinaSeo JaminSmith Andrew JVazey Elena MTremblay Kimberly DMager JesseHardy Jeanne A - Gliomas are highly aggressive brain tumors that pose significant challenges in terms of prognosis and treatment. The precise expression patterns and prognostic significance of necroptosis-related genes (NRGs) in gliomas remain poorly understood, despite necroptosis demonstrating promise as a therapeutic target in cancer. - Source: PubMed
Publication date: 2026/06/11
Wu KaixiangCao LuYang HongchuanSun WenwenWu Kaifu - The razor clam Sinonovacula constricta is an economically important cultivated bivalve. Unlike other bivalve species, S. constricta spawns exclusively at night, suggesting potential endogenous circadian regulation of spawning. To investigate this, we identified circadian clock (period circadian, per) and inflammation-related genes, such as the nuclear factor-κB (nf-κb), inhibitor of NF-κB kinase (iκk), tumor necrosis factor receptor 3 (traf3), B-cell lymphoma-2 (bcl2), and caspase 6 (casp6), which were screened from transcriptome data across four diurnal time points (00:00/ZT16, 06:00/ZT22, 12:00/ZT28, 18:00/ZT34) in S. constricta. Both per and these inflammation-related genes were highly expressed during the light cycle. Furthermore, both their expression levels and corresponding proinflammatory mediator content were significantly higher in the spawning group than the control group. Additionally, interference with the per gene upregulated the expression levels of several inflammation-related genes with predicted E-box motifs in their promoter regions. These findings suggest that the circadian clock may modulate inflammatory responses and proinflammatory factors by rhythmically expressing, thereby influencing spawning behavior in razor clams. This study establishes a foundation for understanding circadian regulatory mechanisms and provides valuable insights into the role of inflammatory responses in molluscan reproduction. - Source: PubMed
Publication date: 2026/05/14
Gu ZefengLu JinzhaoBian QihaoYao HanhanZhang WeiweiDong Yinghui - Caspases are cysteine proteases that regulate programmed cell death. While caspase-6, an executioner caspase, is known for its role in neurodegeneration and cell death, its broader physiological functions remain poorly understood. Our previous study revealed that caspase-6 drives liver injury and fibrosis in metabolic dysfunction-associate steatohepatitis. Here, we report that caspase-6 deficiency protects against high fat diet-induced obesity. Both global and adipocyte-specific caspase-6 knockout mice exhibit increased energy expenditure, reduced adiposity and inflammation, and improved glucose metabolism. Mechanistically, caspase-6 directly cleaves peroxisome proliferator-activated receptor gamma (PPARγ) and its cofactor specificity protein 1 (SP1), thereby suppressing adipose triglyceride lipase (ATGL) expression. Caspase-6 deficiency restores ATGL, enhancing lipolysis and elevating fatty acyl esters of hydroxy fatty acids (FAHFAs), which alleviate inflammation and enhance insulin sensitivity. These findings uncover a novel Casp6-PPARγ/SP1-ATGL axis in adipose tissue and establish caspase-6 as a potential therapeutic target for obesity and insulin resistance. - Source: PubMed
Gupta AbhishekYou WenjingHan LinmengJi JianfeiPan MeixiaHan XianlinSun XiaoliZhao Peng