Ask about this productRelated genes to: Arsb antibody
- Gene:
- ARSB NIH gene
- Name:
- arylsulfatase B
- Previous symbol:
- -
- Synonyms:
- -
- Chromosome:
- 5q14.1
- Locus Type:
- gene with protein product
- Date approved:
- 1986-01-01
- Date modifiied:
- 2015-08-28
Related products to: Arsb antibody
Related articles to: Arsb antibody
- Mucopolysaccharidosis type VI (MPS-VI, Maroteaux-Lamy syndrome) is an inherited progressive disorder caused by the deficiency of the arylsulfatase B enzyme encoded by the ARSB gene. The deficient enzyme activity results in the accumulation of glycosaminoglycans in lysosomes with consequent enlargement of multiple tissues and organs. Due to overlapping clinical symptoms of MPS-VI with other MPS disorders, diagnosis is based on cumulative evaluation of clinical, biochemical, and molecular investigations. The current study recruited two unrelated Pakistani patients suffering from MPS-VI. The diagnosis of the patients was based on clinical assessments and biochemical parameters. We performed direct DNA sequencing of the ARSB gene to screen for pathogenic variants in the patients and their families. All coding exons and intron-exon boundaries were PCR-amplified and subjected to Sanger sequencing. In silico tools were used to predict the pathogenicity of the variants, which were then classified according to the ACMG guidelines. Molecular screening of the patients identified two novel variants: a 2-bp deletion NM_000046.5:c.511_512del and a missense substitution NM_000046.5:c.166G>A. In silico evaluation supported the deleterious effects of the identified variants on the protein function, leading to the MPS-VI phenotype. The study has its significance in expanding the mutation spectrum and may help the affected families in genetic counseling and prenatal diagnosis. To our knowledge, this is the second clinical and molecular genetic report of MPS-VI from Pakistan. - Source: PubMed
Publication date: 2026/08/19
Zubaida BibiBatool HajiraCheema Huma ArshadNaeem Muhammad - Bacillus amyloliquefaciens is a versatile, spore-forming probiotic bacterium with substantial applications in agriculture, industry, and medicine. This research was designed to characterize the probiotic potential of Bacillus amyloliquefaciens BCSIR-AJ isolated from garlic chutney and to evaluate its probiotic and antimicrobial properties. The isolate was acidophilic (optimum pH of 5.0), thermotolerant (55 °C), and halotolerant (10%), and can degrade protein, carbohydrate, and gelatin. The crude extracts of the isolate showed antimicrobial activity against pathogenic Staphylococcus aureus (zone of inhibition (ZOI): 11.6 ± 0.28 mm) and Bacillus cereus (ZOI: 13 ± 0.5 mm), as well as multiple pathogenic fungi (ZOI range from 10.3 ± 0.28 mm - 12.1 ± 0.76 mm). Whole-genome sequence analysis revealed 16 biosynthetic gene cluster regions and genes associated with adhesion (dlt, srtA, tasA), quorum sensing (luxS), stress response (dnaK, clpP, grpE), acid and bile tolerance (atp, bshA), and metal resistance (arsB, czcD, copA, merR) were identified. The isolate showed promising probiotic characteristics, maintaining viability under acidic conditions (pH 2), bile salts (0.3%), and phenol (0.5%), with survival rates of 48.53 ± 1.30%, 96.56 ± 0.008% and 69.15 ± 1.17%, respectively. High survival was also exhibited through simulated stomach duodenum passage (SSDP; 93.29 ± 0.02%) and simulated small-intestine passage (SSIP; 82.25 ± 0.06%). The strain exhibited an antioxidant capacity of 79.15 ± 5.87% and a hydrophobicity of 86.76 ± 2.25%. Additionally, the isolate displayed effective autoaggregation (42.03 ± 0.09) and co-aggregation with B. cereus (51.12 ± 0.10%) and S. aureus (50.39 ± 0.02%). The absence of hemolytic activity and significant biofilm formation further supports its candidacy as a safe probiotic. Overall, B. amyloliquefaciens BCSIR-AJ exhibits a significant antimicrobial activity and promising probiotic potential. - Source: PubMed
Publication date: 2026/07/09
Ayaz MohammedSarkar Md Murshed HasanIslam Mohammad MonerulIslam Muhammad IrfanulKoly Farjana AkterHasan Md RakibulHossain Md SaddamIslam Saiful - The poultry microbiome and virome are integral to the One Health framework, with significant implications for ecosystem and human health, but their responses to arsenic (As) and antimony (Sb) exposure remain overlooked. Here, we conducted a comprehensive metagenomic characterization of the metal resistome, antibiotic resistome, virulome, and virome in poultry feces collected from the world's largest antimony mining area. We found that As and Sb co-contamination was significantly associated with elevated resistance and virulence. The abundance of metal resistance genes (MRGs) was 1.8-fold higher in the high-Sb group than in the low-Sb group (15,022.27 ± 3538.47 vs 8370.24 ± 4502.07 TPM, P = 0.008), with arsR, arsB, and arsC dominating the MRG profiles. Similarly, antibiotic resistance genes (ARGs) abundance was 1.6-fold higher in the high-Sb group than in the low-Sb group (7251.00 ± 1844.34 vs 4478.95 ± 2302.69 TPM, P = 0.026), with multidrug resistance genes being the predominant class (8.09% - 58.48%). Metagenome-assembled genomes (MAGs) analysis and contig analysis suggest co-selection of MRGs, ARGs, and virulence factor genes (VFGs). We identified 100,819 viral contigs clustered into 91,004 viral operational taxonomic units (vOTUs), revealing a highly diverse viral community. Members of Enterobacteriaceae (e.g., Klebsiella) and Enterococcaceae (i.e., Enterococcus) were identified as key drivers mediating resistance and virulence dynamics, acting as resistome supercarriers, opportunistic pathogens, and viral hosts. These findings suggest that As-Sb co-contamination is an overlooked but potentially important driver of poultry antimicrobial resistance and pathogenicity, and highlight potential ecological and public health risks in mining-impacted poultry-associated environments. - Source: PubMed
Publication date: 2026/07/01
Yin ZhipengZhang YuzhuSong ShanjunLi ChengShi JianboYin YongguangCai Yong - In this report, experiments assessed how Arylsulfatase B (ARSB; N-acetylgalactosamine-4-sulfatase) treatment might interact with Pembrolizumab and improve therapeutic responses in melanoma. ARSB acts to remove 4-sulfate groups from chondroitin 4-sulfate (C4S; chondroitin sulfate A; CSA). ARSB is required for the degradation of C4S, identified as an oncofetal, tumor-agnostic antigen. Previous reports showed that in syngeneic B16F10 mouse melanomas, ARSB inhibited progression of subcutaneous and metastatic pulmonary melanomas and improved survival by direct effects on melanoma cells. ARSB enhanced apoptosis, which was mediated by increased expression of Constitutive Photomorphogenic (COP)1, an E3 ubiquitin ligase. Combined treatment by recombinant human ARSB, directed at melanoma cells, and Pembrolizumab, directed at infiltrating cytotoxic lymphocytes, can lead to increased apoptosis by different mechanisms, to declines in metalloproteinases and invasiveness, and to altered expression of cytokines. The apparent synergism between effects of ARSB and Pembrolizumab may further inhibit the progression of melanoma and improve treatment outcomes. - Source: PubMed
Publication date: 2026/06/01
Bhattacharyya SumitO-Sullivan InsugTobacman Joanne K - Human activities have transformed inland waters into reservoirs of co-contamination by heavy metals and persistent organic pollutants, driving microbial adaptation through metal-resistance genes (MRGs) and POP-transformation genes (POPTGs). However, the global biogeography and ecological drivers of these co-occurring functional genes and their hosts remain unresolved. Here, leveraging 1593 metagenomes, we investigate the global distribution, microbial hosts, co-occurrence patterns, and drivers of MRGs and POPTGs in inland waters. Key MRG subtypes (e.g., ruvB, pstB, arsB) and POPTGs (e.g., hdt, linJ, bphA) co-occurred in phylogenetically constrained hosts-predominantly Proteobacteria (e.g., Pseudomonas, Acidovorax)-exhibiting dual resistance to Cr/Cu and transformation of aromatic/chlorinated POPs. The positive correlations linked MRG-POPTG to mobile genetic elements, suggesting horizontal gene transfer accelerates multi-pollutant resistance. Our findings highlight known POPTGs and MRGs occur together, which is ubiquitous in the environment but restricted to a limited number of taxa (approximately 3.8% ratio of the total 4129 non-redundant MAGs). Finally, a global map of MRG-POPTG-carrying MAGs (MPCMs) abundance is generated, where climatic and anthropogenic factors explained MPCMs hot spots in South Asia, Southeast Asia, South America. - Source: PubMed
Publication date: 2026/06/08
Zhu KangyunSun WenweiWang ZheZha YutongQu XueTongWang BinhaoZhang Hangjun