Ask about this productRelated genes to: Arsb antibody
- Gene:
- ARSB NIH gene
- Name:
- arylsulfatase B
- Previous symbol:
- -
- Synonyms:
- -
- Chromosome:
- 5q14.1
- Locus Type:
- gene with protein product
- Date approved:
- 1986-01-01
- Date modifiied:
- 2015-08-28
Related products to: Arsb antibody
Related articles to: Arsb antibody
- Bacteria resist toxic arsenite (As) in their environments by actively pumping the metalloid out of the cell via efflux pumps such as ArsB. However, the mechanism of extrusion remains poorly understood, which hinders the development of engineered bioremediation strategies. We report high-resolution cryo-EM structures of ArsB from the arsenic-tolerant bacterium Leptospirillum ferriphilum. ArsB adopts an inverted two-fold repeat architecture resembling that of other ion transporter (IT) superfamily proteins. Structures determined in the presence of arsenite and antimonite reveal that the metalloid substrates interact with polar residues at the core of the transmembrane domain primarily via hydrogen bonding. Mutagenesis and in vivo functional assays support these interactions. Our ArsB structures represent an 'inward-facing' conformation, where the metalloid-binding site is exposed to the cytoplasm, suitable for metalloid capture. Furthermore, we demonstrate that arsenite resistance conferred by ArsB varies with external pH, supporting that ArsB is a proton (H)-coupled secondary transporter. Mutagenesis, in vivo functional assays, and pK estimation imply that conserved aspartate residues near the metalloid-binding site likely mediate the H-coupling mechanism. Our findings provide structural insights into metalloid recognition and H/metalloid antiport in ArsB, laying a foundation for further elucidation of the molecular basis of toxic metalloid detoxification in bacteria. - Source: PubMed
Publication date: 2026/05/18
Mahajan ShivanshDemirer KemalClemons William MRees Douglas C - Mucopolysaccharidosis type VI (MPS VI) is a rare autosomal recessive lysosomal storage disorder caused by pathogenic variants in ARSB, leading to deficiency of N-acetylgalactosamine 4-sulfatase and accumulation of glycosaminoglycans. Although enzyme replacement therapy (ERT) alleviates systemic symptoms, its efficacy for ocular complications is limited. Because ocular manifestations may require distinct therapeutic approaches, a precise understanding of the underlying retinal pathology is essential. This study aimed to characterize ocular and retinal involvement in MPS VI through clinical and experimental analyses. - Source: PubMed
Sho JunkiNakamura NatsukoOnishi AkishiWatanabe MikiyaSakai DaikiHirami YasuhikoMandai MichikoKurimoto YasuoTakahashi MasayoMaeda Akiko - A river confluence is an important ecosystem to investigate the microbial community and functional profile. Even after the enormous applications of trace elements and antibiotics, their release into the environment causes pollution and selective pressure that facilitate the proliferation and dissemination of resistance genes against antibiotics, metals and biocides among bacterial communities. Metagenomic exploration plays a pivotal role in deciphering riverine ecosystems and offers valuable insights for the mitigation of pollution and the dissemination of resistance genes. Monitoring microbial diversity could aid in identifying various prokaryotes, pathogens, and pollutants, including dyes and their associated resistance genes. Therefore, we aimed to elucidate the occurrence of resistance genes and virulence factors in the microbial community of Saryu River water using high-throughput metagenomics coupled with bioinformatic analyses. The highly dominant antibiotic resistance gene (ARG) types identified were rifampin, tetracycline, macrolide, polymyxin and rifampicin multidrug/efflux. ARGs such as rpoB2, Txr, adeF, tetB(P), and acrB were found to be abundant in Saryu River water. Among the detected MRG subtypes, namely, ruvB and arsB, the most abundant are in water. Further, the biocides against which the resistance was identified were ethidium bromide, triclosan, sodium dodecyl sulfate, etc. Among the virulence factors, tufa, htpB (adherence), Gmd (immune-modulation), cheD (motility), and clpV1 (effector-delivery-system) were found to be highly prevalent. Taxonomic classification revealed that Cyanobateriota, followed by Pseudomonadota (Proteobacteria) and Bacteroidota were the dominant phyla in the river water. Microcystis was the most dominant genus, followed by Desulfomicrobium and Dechloromonas. The present study shows that antibiotics and metals are the major sources of resistance genes development and dissemination in the environment.. Further, this is a preliminary study based on a single composite sample, representing a "snapshot" at a specific time and location. The present study highlights the persistence of ARGs, MRGs, biocides, and virulence factors in Saryu River water and provides valuable baseline data for risk assessment. - Source: PubMed
Publication date: 2026/05/05
Maurya SadanandShukla Awadhesh KumarReddy BhaskarSingh Amit KishoreSingh Vipin KumarTripathi Manikant - Individuals with excessive appearance concerns commonly engage in appearance-related safety behaviors (ARSBs), or behaviors aimed at managing or avoiding negative evaluation of one's physical appearance. Targeting these behaviors can lead to reductions in appearance concerns and related symptoms. However, prior studies have only examined these effects among women scoring above a specific cutoff, excluding men and individuals with lower levels of these concerns. The present study further evaluated the efficacy of this brief ARSB intervention and examined who benefits from this intervention. - Source: PubMed
Patel Tapan ACougle Jesse R - Arsenic contamination in water poses a serious threat to public health, potentially causing various health issues. Therefore, effective remediation technologies are needed to remove arsenic contamination from water. In this study, we developed a whole-cell bioremediation system for simultaneously and efficiently removing As(III) and As(V) from water. By knocking out the arsenic efflux pump gene (arsB) and optimizing the expression of arsenic-binding protein ArsR and arsenate reductase ArsC, we engineered bacterial strains capable of sequestering arsenic within the cells. We systematically compared the remediation performance of the arsenic-inducible (MT022) and constitutive expression (MT047) systems in both free-cell and biofilm states. Both strains achieved over 96% removal of 10 μg/L As(III) and As(V) within 5 h. Even at a higher concentration of 100 μg/L, the constitutive strain MT047 maintained 76.2% removal for As(III) and 55.9% for As(V). While slower than chemical adsorbents, this demonstrates significantly faster kinetics than previous biological systems which typically require 20-24 h to achieve similar removal efficiencies. While both systems performed equally well in the free-cell state, the constitutive expression system MT047 demonstrated superior adsorption capacity in the application of biofilms achieving a 71.8% removal of 100 µg/L As(III) within 24 h. Contrary to conventional favoring surface display systems, our findings revealed that intracellular expression of ArsR and ArsC proved more effective for arsenic bioremediation. To address the application challenges, we have developed a "plug-and-play" biofilm platform that simplifies the operational procedures and provides a safe, efficient solution for treating arsenic-contaminated water. This study offers a promising strategy for comprehensive arsenic pollution control with potential for real-world applications. - Source: PubMed
Publication date: 2026/04/28
Li JiajiaChen ZhiqiangXiong YixiaoZhai JunfengSu YunpengBian YuyingWang ErkangFang Xiaona