Ask about this productRelated genes to: Ppp3cb antibody
- Gene:
- PPP3CB NIH gene
- Name:
- protein phosphatase 3 catalytic subunit beta
- Previous symbol:
- CALNB
- Synonyms:
- CALNA2, CNA2, PP2Bbeta
- Chromosome:
- 10q22.2
- Locus Type:
- gene with protein product
- Date approved:
- 1992-11-09
- Date modifiied:
- 2016-02-10
Related products to: Ppp3cb antibody
Related articles to: Ppp3cb antibody
- Skeletal muscle fiber composition is a key determinant of meat quality and metabolic traits. The early postnatal period constitutes a primary window for muscle fiber transformation. In this study, distinct muscle fiber composition is analyzed by histological and molecular characterization of longissimus dorsi muscle of 2-week-old lean-type and Chinese indigenous pigs. Multi-omics analyses reveal divergence in chromatin states, enhancer landscapes, and promoter activity between breeds. Lean-type pigs show increased chromatin accessibility and enhancer activation at genes involved in oxidative metabolism and myogenesis, whereas Chinese indigenous pigs exhibit enriched regulatory features at glycolytic and biosynthetic loci. Breed-specific SNPs and indels are enriched in a subset of enhancers and potentially influence transcriptional regulation. Higher-order genome architecture further contributes to transcriptional divergence through A/B compartment switching and topologically associating domain boundary remodeling. Promoter-enhancer interaction mapping reveals breed-specific cis-regulatory networks, and transcriptional output correlates with enhancer density. Functional validation identifies a super-enhancer upstream of PPP3CB that recruits MEF2C to activate oxidative fiber programs. In vitro and in vivo perturbation assays confirm that the PPP3CB-MEF2C feedback loop governs muscle fiber-type specification. Collectively, these findings delineate the epigenomic and 3D genomic architecture underlying early muscle fiber characteristics and provide mechanistic insights relevant to improving meat quality. - Source: PubMed
Publication date: 2026/04/17
Zheng ShuailongWu HainanLiu MinWu KunpengHuang ShuntaoAhmed ZulfiqarPius LenoxZhu MengjinXu Dequan - Cardiovascular diseases display strong sex differences. Angiotensin II (AngII) is implicated in this process. The ubiquitously expressed enzymatic beta subunit of calcineurin (PPP3CB), a serine/threonine phosphatase, can mediate pathological effects of AngII in the heart. Our aim was to explore the role of calcineurin in sex-dependent AngII-mediated vascular changes. - Source: PubMed
Nolze AlexanderRabe SindyRuhs StefanieSträtz NicoleQuarch KatjaKöhler ConnyGrossmann Claudia - We previously reported that transcription factor EB (TFEB) plays a crucial role in regulating the ischemic stroke (IS)-mediated dynamic changes of autophagic flux. Protein phosphatase 3 (PPP3) may regulate the transcriptional activity of TFEB. However, the main isoform of the PPP3 catalytic subunit (PPP3C) involved in TFEB activation, the PPP3-binding site in TFEB, and the upstream regulatory mechanism of PPP3 activation after cerebral ischemia are still unknown. Here, we show that the interaction between TFEB and PPP3 catalytic subunit B (PPP3CB), but not PPP3CA, is strengthened after IS. Knockdown of PPP3CB, but not PPP3CA, significantly inhibited the oxygen glucose deprivation (OGD)-induced increase in the transcriptional activity of TFEB, blocked autophagic flux, and exacerbated neuronal death. Furthermore, the YLAVP peptide, which blocks the LxVP motif-binding site of PPP3C, repressed TFEB transcriptional activity and autophagic flux, and exacerbated neuronal death after OGD. Treatment with ML-SI1, which inhibits the lysosomal calcium channel MCOLN1, blocked the OGD-induced enhancement of TFEB transcriptional activity and autophagic flux, and further aggravated neuronal death. These effects were partly reversed by the MCOLN1 agonist ML-SA1. The PPP3 inhibitor cyclosporin A (CsA) abolished the ML-SA1-induced TFEB transcriptional activation and reduced neuronal death. Our findings identify for the first time that MCOLN1-mediated-PPP3CB activation alleviates neuronal damage by promoting TFEB-dependent autophagic flux in permanent cerebral ischemia. The LxVP motif is required for the interaction between PPP3 and TFEB in response to OGD. This study provides an in-depth insight into the mechanisms underlying TFEB-mediated activation of autophagic flux following IS. Schematic diagram showing how MCOLN1-mediated activation of PPP3CB reduces neuronal damage by promoting TFEB-dependent autophagic flux in permanent cerebral ischemia. - Source: PubMed
Publication date: 2026/03/24
Liu Shi-QiLiu Yue-YangFu Xiao-XiaoCui Pei-RuiWang XinWang Sai-QianWu Chun-FuWang Li-HuiLuo JingYang Jing-Yu - Lysosomal adaptation through the endo-lysosomal damage response (ELDR) enables cancer cells to evade lysosome-targeted therapies. Here, we identified the flavonoid compound V8 as a first-in-class ELDR disruptor that eliminated cancer cells by sabotaging lysosomal resilience. Mechanistically, V8 bound lysosomal HSP70 via hydrogen bonding, destabilizing its interaction with bis(monoacylglycero)phosphate (BMP) and triggering pathological sphingomyelin (SM) accumulation. SM overload allosterically inhibited TRPML1, blocking calcineurin PPP3CB activation and subsequent TFEB dephosphorylation. This dual perturbation: enhanced lysophagy and failed TFEB-driven biogenesis drove catastrophic lysosomal bankruptcy. Crucially, V8 bypassed canonical ELDR activation: unlike lysosomotropic agent LLOMe, it induced global membrane remodeling rather than focal perforations, avoiding Ca²⁺-dependent endosomal sorting complexes required for transport (ESCRT) repair. Genetic validation using HSP70-knockout and point mutation models confirmed target specificity, while SM synthase inhibition rescued TRPML1 activity and mitigated apoptosis. Tumor-selective efficacy arose from malignant cells' heightened SM dependency and lysosomal HSP70 reliance, sparing normal counterparts. Our work established HSP70-BMP-ASM axis disruption as a strategy to subvert lysosomal homeostasis, providing a blueprint for next-generation lysosome-targeting agents that exploit lipid-mediated channelopathy to sensitize cancer cells to lysosomal damage. - Source: PubMed
Publication date: 2025/12/05
Chen HongyuZhu MengyuanZhao ZiyingJiang YuexinWang HaidiLiu YuChen YanLi HuiXun ChenHui Hui - Pishan Red Sheep and Hu Sheep are sheep breeds with exceptional reproductive characteristics. To investigate the similarities and differences in the expression of reproduction-related genes between these two breeds, this study utilized transcriptome sequencing to identify differentially expressed lncRNAs and mRNAs in ovarian tissues during estrus in Hu Sheep and Pishan Red Sheep carrying and genotypes. Furthermore, we explored their potential impacts on fertility. Transcriptome sequencing of ovarian tissues generated 204.58 Gb of clean data. Bioinformatics analysis identified 34,651 lncRNAs, with differential expression analysis revealing 1,481 differentially expressed mRNAs and 698 differentially expressed lncRNAs. Differentially expressed RNAs associated with reproductive performance trends were screened through expression trend analysis. Functional enrichment analysis of target genes for these mRNAs and lncRNAs revealed significant enrichment in KEGG pathways such as "Cytokine-cytokine receptor interaction," "Hippo signaling pathway" and "MAPK signaling pathway" Key candidate mRNAs were identified, including , , , , , , , , , , and . Additionally, critical regulatory relationships between lncRNAs and mRNAs were uncovered. For example, exhibited high expression in genotype Pishan Red Sheep and may act as a hub regulator in follicular selection and hormonal responses by cis-regulating and trans-regulating , , may regulate genes such as and , potentially participating in the modulation of the ovarian tissue remodeling microenvironment. In contrast, cis-regulates to modulate the granulosa cell proliferation and differentiation process. The specifically highly expressed in Hu Sheep may be involved in maintaining ovarian stromal cell homeostasis through trans-regulation of and , and target and respectively, suggesting their potential roles in cell cycle regulation and oocyte maturation. These findings provide important molecular mechanisms and potential regulatory targets for improving reproductive performance in sheep. - Source: PubMed
Publication date: 2025/09/24
Muhetaer AisimaGong GaoYe YaoyangKuxitaer AyipareZhu MengtingLi QifaDu XingSulaiman Yiming