Ask about this productRelated genes to: ARHGAP20 antibody
- Gene:
- ARHGAP20 NIH gene
- Name:
- Rho GTPase activating protein 20
- Previous symbol:
- -
- Synonyms:
- KIAA1391
- Chromosome:
- 11q22.3-q23.1
- Locus Type:
- gene with protein product
- Date approved:
- 2003-12-05
- Date modifiied:
- 2018-02-13
Related products to: ARHGAP20 antibody
Related articles to: ARHGAP20 antibody
- Cell migration requires the precise coordination of signaling pathways that regulate cytoskeletal dynamics and adhesion turnover. Rho GTPase-activating proteins (RhoGAPs) play critical roles in shaping these processes by controlling the spatial and temporal activity of small GTPases. ARHGAP20 is a RhoA-specific GAP, a downstream target of the Ras-related GTPase Rap1, and has been implicated in cancer cell motility, yet its functional role in coordinating migration-associated signaling remains poorly understood. Here, we investigated the role of ARHGAP20 in cell migration and its impact on the coordination between adhesion- and contractility-associated signaling pathways regulated by Rap1A and RhoA respectively. Using loss-of-function approaches in MTLn3 cells, we show that depletion of ARHGAP20 impairs both directed and random migration, leading to reduced cell velocity and displacement, and increased cell adhesion. To explore the underlying signaling mechanisms, we developed a genetically encoded FRET biosensor to monitor Rap1A activity and combined it with a near-infrared RhoA biosensor to simultaneously analyze their spatiotemporal dynamics in living cells. We found that Rap1A and RhoA activities are negatively coordinated during leading-edge dynamics and that ARHGAP20 depletion enhances this local anticorrelation. To further define where ARHGAP20 regulates Rap1A-RhoA signaling, we applied a microdomain-based analytical framework to quantify local signaling clusters. This analysis revealed that ARHGAP20 selectively modulates Rap1A-RhoA coordination outside focal adhesion regions, while leaving signaling correlations and overlap within focal adhesions unchanged. Subcellular localization analyses further reveal that ARHGAP20 is largely excluded from focal adhesions but associates with microtubules, the endoplasmic reticulum, the Golgi apparatus, and multiple Rab-positive vesicular compartments, supporting a trafficking-dependent mechanism for its spatial targeting. Together, our results identify ARHGAP20 as a regulator of cell migration that modulates the coordination between Rap1A and RhoA signaling through intracellular vesicular trafficking, highlighting the GAP's role in organizing the spatial coupling between signaling pathways in adhesion-associated regions required for efficient cell migration. - Source: PubMed
Publication date: 2026/07/20
Pagano SandraSanchez CollineCox-Cammer NatashaBhalla Ravi MRavishankar RoshanSegura-Villalobos DeisyMuesch AnneAguirre-Ghiso Julio ADanuser GaudenzHodgson Louis - To investigate the biological behavior, differentiation ability, and differential gene expression of lymph node mesenchymal stem cells (MSCs) in patients with diffuse large B-cell lymphoma (DLBCL) and reactive lymphoid hyperplasia (RLH), providing a theoretical basis for clinical chemotherapy resistance. - Source: PubMed
Ma Yu-ShuoLiu Zhi-HeSun YangZhang Yu-HangWang Wen-QiuWang Li-ShengZhao Xia - To investigate the targets and mechanisms of 7-hydroxyethyl chrysin (7-HEC) in prevention and treatment of high-altitude cerebral edema (HACE) in rats. - Source: PubMed
Zhang DongmeiLi XiaolinYang ChenyuJing LinlinHe LeiMa Huiping - Circular RNAs (circRNAs) have emerged as critical regulators of biological processes, but their roles in avian muscle development remain less explored. Here we characterize circMKNK2, a novel circRNA derived from the MKNK2 gene, which is highly expressed in slow-growing Silky chickens compared to fast-growing broilers. Functional studies demonstrate that circMKNK2 acts as a sponge for miR-15a, with overexpression inhibiting myoblast proliferation, differentiation, apoptosis, and glucose metabolism, while miR-15a knockdown produces similar effects except for enhanced glucose uptake. RNA-seq analysis identified 2189 differentially expressed genes regulated by circMKNK2 in chicken primary myoblasts, including key targets of the circMKNK2/miR-15a axis such as PIK3R1 (a core node regulating PI3K-Akt signaling), BHLHE41, KANK1, and ARHGAP20. Pathway analysis revealed modulation of myogenesis through Calcium signaling pathway, ECM-receptor interaction, Neuroactive ligand-receptor interaction and immune-related pathways (Toll-like receptor, cytokine-cytokine receptor interactions). Further analysis highlighted the circMKNK2/miR-15a axis's role in suppressing myogenesis through transcriptional regulation of key factors (e.g., SOX7, MAF) and metabolic reprogramming. Unlike pro-myogenic circRNAs, circMKNK2 uniquely inhibited muscle development and glucose metabolism, suggesting its involvement in breed-specific phenotypic differences. This study provides insights into circRNA-mediated regulation of muscle biology and offers potential targets for improving poultry production through genetic and metabolic modulation. - Source: PubMed
Publication date: 2025/05/14
Li HuihongZhang LinJiao JingyaZhang HuaiyongSi XuemengHuang YanqunChen Wen - To investigate the role of Rho GTPase-activating protein 21 (ARHGAP21) in regulating the migration and metastasis of non-small cell lung cancer (NSCLC) cells. - Source: PubMed
Xie ZLiu LFang JZhong XLin JChen F