Ask about this productRelated genes to: Ppp2r5e antibody
- Gene:
- PPP2R5E NIH gene
- Name:
- protein phosphatase 2 regulatory subunit B'epsilon
- Previous symbol:
- -
- Synonyms:
- B56E, B56epsilon
- Chromosome:
- 14q23.2
- Locus Type:
- gene with protein product
- Date approved:
- 1996-05-08
- Date modifiied:
- 2017-04-05
Related products to: Ppp2r5e antibody
Related articles to: Ppp2r5e antibody
- Phosphorylation of alpha-synuclein (αsyn) at serine 129 (PS129) marks aggregates in synucleinopathies but also occurs physiologically, potentially signaling protein interactions during neuronal activity. Technical barriers, including postmortem dephosphorylation, have hindered the study of physiological PS129 in the human brain. Using biotinylation by antibody recognition (BAR) on surgically resected temporal lobectomy tissues (without post-mortem interval), we mapped physiological PS129 and total αsyn interactomes. BAR identified 1,095 interactions with 513 αsyn-specific, 524 shared, and 58 PS129-specific, mostly associated with vesicles at presynaptic nerve terminals. PS129-specific interactions were uniquely associated with postsynaptic density proteins SHANK1/3, DLGAP1-4, DLGAP1-3, and DLG2-4, as well as nuclear-associated proteins HUWE1, HNRNPM, RBM14, ITCH, OGT, PHF24, and PPP2R5E. Fluorescent staining confirmed physiological PS129 proximal to dendrites and within the nucleus. Confirmation in healthy cynomolgus macaques (62% αsyn and 41% PS129 overlap) demonstrated that the interactomes were physiological rather than disease- or aggregate-associated. We conclude that physiological PS129 plays a unique and underappreciated role in postsynaptic neurons extending from the postsynaptic active zone to the nucleus. These interactomes benchmark normal αsyn biology, illuminating the transition to synucleinopathy pathology. - Source: PubMed
Publication date: 2025/11/27
Choi Solji GDuvernay Jayda BBahrami AtousaTittle TylerSani SepehrKordower Jeffrey HMuller ScottKillinger Bryan A - Head and neck cancer is among the top ten cancers worldwide, with most lesions in the oral cavity. Oral squamous cell carcinoma accounts for more than 90% of all oral malignancies and is a significant public health concern. Circular RNA and micro RNA, as non-coding RNA, plays an important role in the development of tumor transmission. - Source: PubMed
Publication date: 2025/09/08
Liu XueXiong HuiWang XuefengLi LinaLiu ChangHuo FengTao Yadong - Dynamics of protein phosphorylation are regulated by the interplay of kinases and phosphatases. Current mass spectrometry-based phosphoproteomic approaches are extremely powerful in identifying and quantifying tens of thousands of phosphosites in single biological samples. However, whereas the mapping of phosphosites is successfully automated supporting high sample throughput, the characterization of responsible kinases and phosphatases still largely depends on laborious protein biochemical assays. To show direct (de)phosphorylation events, in vitro kinase or phosphatase assays using single substrates or peptide arrays are often used. Here, we describe the development of an in vitro phosphatase assay using whole proteome under native conditions as input. We employ this approach to study the PP1 and PP2A target repertoire, characterizing thousands of potential target sites. Focusing on PPP2R5E/B56ε-containing complexes, we combine in vitro with in vivo phosphoproteomics to characterize bona fide target sites, which highlight the role of PP2A in regulating stress granule assembly. - Source: PubMed
Publication date: 2025/06/19
Brunner MelanieHu ZehanElkhaligy HeidyLampo GloriaRoubaty CaroleVionnet ChristineSankar Devanarayanan SivaMcIlwain Sean JKaeser-Pebernard StéphanieXing YongnaDengjel Jörn - Reprogramming of aged donor tissue cells into induced pluripotent stem cells (A-iPSC) preserved the epigenetic memory of aged-donor tissue, defined as genomic instability and poor tissue differentiation in our previous study. The unbalanced expression of RNA exosome subunits affects the RNA degradation complex function and is associated with geriatric diseases including premature aging and cancer progression. We hypothesized that the age-dependent progressive subtle dysregulation of EXOSC2 (exosome component 2) causes the aging traits (abnormal cell cycle and poor tissue differentiation). We used embryonic stem cells as a tool to study EXOSC2 function as the aging trait epigenetic memory determined in A-iPSC because these aging traits could not be studied in senesced aged cells or immortalized cancer cells. We found that the regulatory subunit of PP2A phosphatase, PPP2R5E, is a key target of EXOSC2 and this regulation is preserved in stem cells and cancer. - Source: PubMed
Publication date: 2025/04/05
Skamagki MariaZhang ChengHacisuleyman EzgiGalleti GiuseppeWu ChaoVinagolu Rajasekhar KCha HyoKyeongAta DenizKim JongjinWeiskittel TaylorDiop MameAung ThiriDel Latto MichaelKim Amanda SLi ZhuoningMiele MatthewZhao RuiTang Laura HHendrickson Ronald CRomesser Paul BSmith Joshua JGiannakakou ParaskeviDarnell Robert BBott Matthew JLi HuKim Kitai - Kinectin 1 () is a membrane protein involved in intracellular organelle motility. However, the role of in human pan-cancer lacks systematic analysis and evaluation. The aim of this study is to evaluate the expression profile and clinical value in human cancers by performing a pan-cancer analysis of . - Source: PubMed
Publication date: 2024/11/27
Ouyang YanShen YuLai ShengmingHuang HaiyanHuang YongshengRen Shuwei