Ask about this productRelated genes to: Twf1 antibody
- Gene:
- TWF1 NIH gene
- Name:
- twinfilin actin binding protein 1
- Previous symbol:
- PTK9
- Synonyms:
- A6
- Chromosome:
- 12q12
- Locus Type:
- gene with protein product
- Date approved:
- 1997-12-17
- Date modifiied:
- 2016-04-25
Related products to: Twf1 antibody
Related articles to: Twf1 antibody
- Forty-five fungal strains from decomposing wood, representing eight genera, were isolated. Among them, 27 cellulolytic strains were identified. The genera Trichoderma, Penicillium, and Phanerochaete (6, 2, and 4 isolates, respectively) demonstrated the highest cellulase production. The isolates TW-25, TW-28, and TW-33 exhibited superior enzyme activities, CMCase (5.4-6.5 U/mL), FPase (3.2-3.8 U/mL), pNPCase (2.6-3.0 U/mL), and pNPGase (3.5-4.0 U/mL) and were selected for further study. Internal transcribed spacer (ITS) region sequencing, combined with phenotypic characteristics, identified these strains as Trichoderma sp. TW-25, Trichoderma sp. TW-28, and Penicillium sp. TW-33. Maximum protoplast release was observed in Trichoderma sp. TW-25 (3.6 × 10⁶ protoplasts/mL), followed by Trichoderma sp. TW-28 (3.0 × 10⁶ protoplasts/mL) and Penicillium sp. TW-33 (2.8 × 10⁶ protoplasts/mL). Fusion frequencies were 2.8 × 10⁻³ for TW-25 × TW-28, 2.0 × 10⁻³ for TW-25 × TW-33, and 1.8 × 10⁻³ for TW-28 × TW-33. A total of 13 colonies obtained from TW-25 × TW-28, and 18 from intergeneric fusions (10 from TW-25 × TW-33 and 8 from TW-28 × TW-33). The cellulase activity of the fusants TWF1/1, TWF1/6, and TWF2/5 was the same as TW-25 and the fusants TWF1/3 and TWF3/2 the same as TW-28 while none of the fusants had the cellulase activity of TW-33. Fusants differed from their parental strains in their DNA content (3.25-3.65 µg/mg dry weight) and showed high cellulase activities in general. Among them, TWF1/10 demonstrated the highest enzymatic activity, producing CMCase, FPase, pNPCase, and pNPGase (10.5, 6.5, 5.8, and 7.5 U/mL), respectively, followed by TWF1/13, TWF2/8, TWF2/10, and TWF3/8. DNA banding patterns of TWF1/10, TWF1/13, TWF2/8, TWF2/10, and TWF3/8, analyzed using four RAPD and three ISSR primers, differed from their parental strains, except for ISSR-3 with fusants TWF1/10 and TWF1/13. These variations underscore the effectiveness of interspecific and intergeneric protoplast fusion. The supernatant of the hybrid strain TWF1/10 was concentrated and purified via ultrafiltration, and SDS-PAGE and zymogram assays confirmed its cellulase activity using CMC as the substrate. - Source: PubMed
Publication date: 2025/10/24
Alsarraf Mohammad JAl-Wahaibi Aisha S MStephenson Steven LAlshaikh Najla AAmeen Fuad - To investigate the molecular mechanisms underlying how Twinfilin actin-binding protein 1 (TWF1) drives the malignant progression of head and neck squamous cell carcinoma (HNSCC). - Source: PubMed
Publication date: 2025/10/14
Yang YingshunYang KaichengPeng ShixiongMan ShashaChen He - Reelin is an extracellular glycoprotein essential for neuronal migration, spine development, and synaptic plasticity. Impaired reelin signaling is linked to neurological disorders, including schizophrenia and autism. While reelin mutant (reeler) mice exhibit behavioral deficits associated with impaired spine formation, the underlying molecular mechanisms remain unclear. We identified Twinfilin-1 (Twf1) as a downstream effector of reelin signaling via phosphoproteomic analysis, based on its reduced tyrosine phosphorylation in reeler mice. We found that Src regulated Twf1 phosphorylation at tyrosine 309, and reelin stimulation increased Twf1 phosphorylation in neurons, an effect blocked by the Src inhibitor PP2. A phospho-resistant Twf1 mutant (Twf1 Y309F) showed reduced capping protein binding and a lower F/G-actin ratio. Twf1 mice exhibited cognitive deficits, reduced spine density, smaller spine head size, and a decreased F/G-actin ratio in synaptosomes. These findings highlight Twf1 phosphorylation as a key component of reelin signaling involved in actin remodeling and spine development. - Source: PubMed
Publication date: 2025/10/10
Dong GeyaoMori DaisukeMatsuzaki TetsuoTanaka RinakoItoh NorimichiMatsui TakaakiSato AyatoArioka YukoOkumura HirokiFukaya RyotaKuba HiroshiNagai TakuNabeshima ToshitakaIkesue HiroakiKohno TakaoHattori MitsuharuKaibuchi KozoOzaki NorioMizoguchi HiroyukiYamada Kiyofumi - The Tswana goat, a key component of Botswana's livestock, is renowned for its resilience and adaptability to low-resource environments. The objective of this study was to use homozygosity (ROH) and supplementary methods (F, iHS, xp-EHH, Rsb) to identify selection signatures and inbreeding pattern of the indigenous Tswana goat. A total of 216 goats were used, that is, Tswana (n = 114) from three agroecological regions in Botswana and Boer (n = 102), a reference population from South Africa. After quality control, 216 animals and 49 732 single-nucleotide polymorphisms were available for analysis. Tswana goats exhibited an average ROH length of 2.20 Mb and 85.71 ROH per goat, while Boer goats had longer, fewer ROH, averaging 155.96 Mb and 3.14 ROH per goat. Tswana goats had a lower inbreeding coefficient (F = 0.08) compared to Boer goats (F = 0.13). Significant ROH hotspots were found on chromosomes 12, 6, and 5 in Tswana goats and on chromosomes 24, 14, 9, 8, and 6 in Boer goats, with 27 annotated genes identified. Multiple selection signature detection methods detected genes such as PUS7L, ADAMTS20, TWF1, PRICKLE1 YAF2 and GXYLTI. Key genes associated with reproductive fitness (ATP12A, RNF17), immune response (IL17D, PARP4), coat colour variation (ADAMTS20), and milk synthesis (TWF1) were highlighted in Tswana goats, reflecting adaptive responses to environmental pressures. The study provides insights into the genetic adaptations and historical breeding of Tswana goat. This knowledge is crucial for implementing effective conservation strategies and enhancing the resilience of indigenous goat populations. By prioritising the genetic study of these goats, Botswana can ensure the sustainability of its unique livestock resources, promoting both food security and rural development in the region. - Source: PubMed
Publication date: 2025/07/02
Chalebgwa A BMonau P IRaphaka KHadebe KKgwatalala P MNsoso S J - Lung adenocarcinoma (LUAD), the most prevalent subtype of non-small cell lung cancer, is a significant cause of cancer-related mortality due to late-stage diagnoses and limited treatment options. Identifying effective biomarkers is crucial for improving prognosis and therapeutic strategies. miR-30a-5p has emerged as a potential tumor suppressor in LUAD, yet its exact role remains unclear. In this study, we analyzed three miRNA expression datasets from the GEO database and confirmed miR-30a downregulation in LUAD through TCGA and qRT-PCR validation. We identified MYBL2, TWF1, GALNT7, and PFN2 as oncogenic targets of miR-30a by integrating miRNA target predictions with gene expression data. Expression and survival analyses revealed an inverse relationship between miR-30a and these targets, with high expression levels correlating with poor patient outcomes. Functional enrichment analysis highlighted pathways in cell cycle regulation and actin cytoskeleton organization. Our findings underscore the significance of the miR-30a regulatory network in LUAD progression, positioning miR-30a and its targets as promising prognostic biomarkers and therapeutic targets. - Source: PubMed
Publication date: 2025/07/08
Sahin YunusAltan ZekiyeSaadat Khandakar A S MIkeda Masa-AkiArslan Ahmet