Ask about this productRelated genes to: Harbi1 antibody
- Gene:
- HARBI1 NIH gene
- Name:
- harbinger transposase derived 1
- Previous symbol:
- C11orf77
- Synonyms:
- FLJ32675
- Chromosome:
- 11p11.2
- Locus Type:
- gene with protein product
- Date approved:
- 2006-05-10
- Date modifiied:
- 2014-11-19
Related products to: Harbi1 antibody
Related articles to: Harbi1 antibody
- Prothrombin gene mutations can be associated with either a thrombotic or a bleeding risk. Genomic studies and coagulation workup can provide valuable information to better understand their clinical importance. - Source: PubMed
Publication date: 2026/02/02
Siegemund AnnelieSiegemund ThomasBönigk HagenSchlosser KristinaKonn KatjaKeil SabinePetros Sirak - Pugionium cornutum is a unique herbaceous plant endemic to the deserts of northwest China. Known for its extreme resilience to drought and wind, it is valued for both culinary and medicinal uses, earning it the epithet "desert treasure." HARBI1 is a protein involved in abiotic stress response and plant growth regulation; however, its role in the stress adaptation of P. cornutum remains unclear. In this study, we analyzed transcriptome data from drought-stressed P. cornutum and identified 13 members of the HARBI gene family. Among these, PcHARBI1-5 was selected as a target due to its significant downregulation in both roots and leaves. Using overexpression constructs (OE) and a knockout mutant (KO-1) of its closest homolog in Arabidopsis thaliana, we demonstrated that PcHARBI1-5 is primarily localized in the nucleus. Overexpression of PcHARBI1-5 in Arabidopsis resulted in reduced germination rates and stronger inhibition of root growth under drought, salt, and heavy metal stresses. Measurements of key physiological indicators revealed that transgenic lines exhibited significantly lower activities of antioxidant enzymes (SOD, POD, CAT), reduced proline content, increased membrane damage, and elevated HO accumulation. In contrast, mutant plants displayed enhanced stress tolerance. These results indicate that PcHARBI1-5 acts as a negative regulator of abiotic stress responses in Arabidopsis, and that its knockout improves tolerance under adverse conditions. - Source: PubMed
Publication date: 2025/10/10
Zhao YunweiShang KaifanWang Ping - Harbinger transposase-derived 1 proteins (HARBI1s) play important roles in plant growth, development, and response to abiotic stress. OsHARBI1-1 has been identified as a gene encoding HARBI1-1 protein in rice and has been shown to be responsive to Cadmium (Cd) stress. However, the function of OsHARBI1-1 protein under heavy metal stress remains unclear. In this study, the function of a novel rice Cd-responsive gene, OsHARBI1-1, under Cd stress was characterized by heterologous expression in yeast. The heterologous expression OsHARBI1-1 conferred yeast with increased tolerance to Cd. In addition, the yeast cells expressing OsHARBI1-1 exhibited enhanced tolerance to Congo red and exhibited an increase in cell wall thickness under Cd stress, suggesting a potential correlation between increased Cd tolerance and cell wall thickness in the transgenic yeast. When OsHARBI1-1 was expressed in ∆yap1 or ∆yap1∆ycf1 yeast mutants, there was no significant difference in the tolerance of transgenic yeast to Cd and Congo red, as well as in cell wall thickness compared to the control. Meanwhile, the expression of cell wall integrity (CWI) genes and catalase genes in transgenic yeast was up-regulated in a YAP1-dependent manner under Cd or Congo red stress. The above facts supported the inference that OsHARBI1-1 may counteract Cd toxicity by enhancing the expression of YAP1, thereby increasing the thickness of the cell wall and activating the expression of catalase genes. - Source: PubMed
Shi YangJiang NanJiang WenjunChen JiWu LijuanJiao YuanZhou YingxuWu LongyingHuang YanyanLi DeqiangKumar VinodHu BinhuaHuang Jin - As one of the major food crops in the world, rice is vulnerable to cadmium (Cd) pollution. Understanding of the molecular mechanisms of Cd uptake, transport and detoxification in rice is essential for the breeding of low-Cd rice. However, the molecular mechanisms underlying the response of rice to Cd stress remains to be further clarified. - Source: PubMed
Publication date: 2023/11/11
Jiang NanShi YangLi MingyuDu ZhiyeChen JiJiang WenjunHuang YanyanZhong MinYang JuHu BinhuaHuang Jin - pGhERF105 and pGhNc-HARBI1 promoters are highly responsive to CBW infestation and exhibit strong activity in vegetative and reproductive tissues, increasing their potential application in GM crop plants for pest control. The main challenge to cotton (Gossypium hirsutum) crop productivity is the constant attack of several pests, including the cotton boll weevil (CBW, Anthonomus grandis), which uses cotton floral buds for feeding and egg-laying. The endophytic nature of the early developmental stages of CBW makes conventional pesticide-based control poorly efficient. Most biotechnological assets used for pest control are based on Bacillus thurigiensis insecticidal Cry toxins or the silencing of insect-pest essential genes using RNA-interference technology. However, suitable plant promoter sequences are required to efficiently drive insecticidal molecules to the target plant tissue. This study selected the Ethylene Responsive Factor 105 (GhERF105) and Harbinger transposase-derived nuclease (GhNc-HARBI1) genes based on available transcriptome-wide data from cotton plants infested by CBW larvae. The GhERF105 and GhNc-HARBI1 genes showed induction kinetics from 2 to 96 h under CBW's infestation in cotton floral buds, uncovering the potential application of their promoters. Therefore, the promoter regions (1,500 base pairs) were assessed and characterized using Arabidopsis thaliana transgenic plants. The pGhERF105 and pGhNc-HARBI1 promoters showed strong activity in plant vegetative (leaves and roots) and reproductive (flowers and fruits) tissues, encompassing higher GUS transcriptional activity than the viral-constitutive Cauliflower Mosaic Virus 35S promoter (pCaMV35S). Notably, pGhERF105 and pGhNc-HARBI1 promoters demonstrated more efficiency in driving reporter genes in flowers than other previously characterized cotton flower-specific promoters. Overall, the present study provides a new set of cotton promoters suitable for biotechnological application in cotton plants for pest resistance. - Source: PubMed
Publication date: 2022/06/04
de Moura Stéfanie MenezesFreitas Elinea OliveiraRibeiro Thuanne PiresPaes-de-Melo BrunoArraes Fabrício B MMacedo Leonardo Lima PepinoPaixão Joaquin F RLourenço-Tessutti Isabela TArtico Sinarada Cunha Valença DavidSilva Maria Cristina Mattarde Oliveira Antonio CAlves-Ferreira MarcioGrossi-de-Sa Maria Fatima