Ask about this productRelated genes to: Lig3 antibody
- Gene:
- LIG3 NIH gene
- Name:
- DNA ligase 3
- Previous symbol:
- LIG2
- Synonyms:
- LIG3alpha
- Chromosome:
- 17q12
- Locus Type:
- gene with protein product
- Date approved:
- 1991-05-09
- Date modifiied:
- 2018-08-14
Related products to: Lig3 antibody
Related articles to: Lig3 antibody
- Precise regulation of enzyme recruitment during Okazaki fragment maturation (OFM) is essential for faithful and efficient lagging-strand DNA synthesis. Emerging evidence suggests that PARP1 contributes to OFM yet its specific functions remain unclear. Here, we define context-dependent functions of PARP1 during OFM. Under physiological conditions, PARP1 co-localizes with PCNA in early S phase and restrains Pol δ-PCNA- mediated strand-displacement DNA synthesis, thereby preventing the formation of long 5' flaps, which is refractory to FEN1 cleavage. On the other hand, in LIG1-deficient cells, in which DNA nicks and unexpectedly long 5' flaps accumulate, PARP1 promotes the recruitment of LIG3 to catalyze OF ligation and DNA2 to facilitate long 5' flap processing. Collectively, our findings uncover previously unrecognized roles of PARP1 in regulating 5' flap dynamics to ensure efficient OFM and cell viability. - Source: PubMed
Publication date: 2026/04/30
Shi GuojunWang YixingYan YaoLi KejiaoMa LingzhiLei YiWang YingyingManriquez NancyZhou MianZha ShanZheng LiShen Binghui - This work investigates how the concepts of solvate ionic liquids (SILs) and water-in-salt (WIS) electrolytes can be combined to create hybrid electrolyte systems. We examine the neat SIL [Li(G3)][NTf], composed of a solvate cation and an anion, as well as its water-modified analog containing an equimolar amount of added water, using both molecular dynamics simulations and experiments. Introducing water markedly reduces the high viscosity of the neat SIL while substantially enhancing ionic conductivity. Structurally, each cationic complex incorporates on average a single water molecule, resulting in highly dispersed water and the absence of extended water networks. We refer to such systems as water-in-solvate-ionic-liquid (WISIL) electrolytes. Owing to the strongly coordination-dominated lithium environment, the WISIL retains a wide electrochemical stability window, decreasing only slightly from over 5 V in the neat SIL to 4.9 V at ambient conditions. - Source: PubMed
Philipp Jule KristinPaschek DietmarKruse LennartSurkus Annette-EnricaAustrup BennetSchönhoff MonikaLudwig Ralf - Ultraviolet exposure is the main environmental risk factor for non-melanoma skin cancers (NMSCs), but genetic susceptibility also contributes to variability in disease burden among individuals. - Source: PubMed
Publication date: 2026/04/11
Campana IreneMinafò Ylenia AuraScaglione Giovanni LucaGiovanardi GiuliaCosio TerenzioLanna CaterinaArtosi FabioLambiase SaraValente CarolaBartolocci ValeriaMorelli MartinaAlbanesi CristinaPalese EnzoMinieri MarilenaGiovannelli AlfredoAvitabile DanieleSalcedo Nidia MargotMastroeni SimonaMazzanti CinziaAbeni DamianoCampione ElenaFania LucaDellambra Elena - Radiation therapy (RT) is one of the most important strategies for killing cancer cells and shrinking tumors. However, in colorectal cancer, the application of radiotherapy is restricted due to radioresistance. Therefore, exploring the detailed mechanisms of radioresistance may improve patient responses to irradiation and enhance survival rates. Here, we selected SW480 and SW620 cells for X-ray radiotherapy. To determine the D10 (the dose that reduces cell survival to 10%) of colorectal cancer cells to X-ray, we used a colony formation assay, and 6 Gy was chosen for further experiments. Through TCGA, we found that miR-142-3p was upregulated in colorectal cancer cells. We then transfected miR-142-3p into colorectal cancer cells, constructing stable lines. We discovered that miR-142-3p promoted the radioresistance of colorectal cancer cells, and in vivo assays also demonstrated the same effect. Mechanistically, we identified FOXO4 as the direct target of miR-142-3p by Dual Luciferase Reporter Assay. RNA sequencing revealed that miR-142-3p enhanced DNA damage repair. Western blot analysis determined that the NHEJ pathway was involved in regulating this process, including DNA-PKcs, Ku80, Rad50, NBS1, MRE11, XRCC1, LIG3, et al. Together, our findings suggest that miR-142-3p plays a radioresistant role in colorectal cancer and reveals a potential therapeutic target to enhance the effectiveness of radiotherapy. - Source: PubMed
Publication date: 2026/04/03
Wang XiHua Wei-XiLiu YingLi Hong-Dan - - Source: PubMed
Publication date: 2026/03/31
Ravens UrsulaCote AnthonyPeyronnet Rémi