Ask about this productRelated genes to: GCLC antibody
- Gene:
- GCLC NIH gene
- Name:
- glutamate-cysteine ligase catalytic subunit
- Previous symbol:
- GLCLC, GLCL
- Synonyms:
- GCS
- Chromosome:
- 6p12.1
- Locus Type:
- gene with protein product
- Date approved:
- 1993-11-24
- Date modifiied:
- 2019-04-23
Related products to: GCLC antibody
Related articles to: GCLC antibody
- Osteoporosis is an age-related metabolic bone disorder characterized by an imbalance between bone resorption and formation. Natural polyphenols have gained attention as potential complementary strategies for its prevention. In this study, we investigated the effects of a sustainable, polyphenol-rich extract from red grape pomace (GPE) on human mesenchymal stem cell (MSC) fate and its underlying mechanisms of action. We found that GPE significantly promoted osteogenic differentiation while suppressing adipogenic differentiation in canonical bone marrow-derived MSCs (BMSCs). This biological effect was preserved in adipose tissue-derived MSCs (AdMSCs) obtained from elderly patients (>65 years) at high cardiovascular risk. Mechanistically, GPE downregulated adipogenic markers (PPARγ, CD36 and FABP4) and enhanced osteogenic markers (RUNX2, ALP, OSX, BMP-2, OPN, COL1A1 and OCN). Moreover, GPE activated NRF2-dependent redox signaling, as evidenced by increased NRF2 nuclear translocation and transcriptional activity. Accordingly, GPE treatment significantly upregulated, or consistently increased, the expression of multiple NRF2 target genes, including HO-1, GPX, CAT, GCLC, and NQO1. Importantly, pharmacological inhibition of NRF2 attenuated GPE-induced ALP activity, confirming NRF2 as a key mediator of its osteogenic effects. Overall, grape pomace-derived polyphenols act as upstream modulators of redox-sensitive and osteogenic transcription factors, rebalancing MSC differentiation toward osteogenesis and mitigating age-related bone fragility. - Source: PubMed
Publication date: 2026/05/01
Calabriso NadiaMassaro MarikaQuarta StefanoSiculella LuisaSantarpino GiuseppeVerri TizianoGerardi CarmelaGiovinazzo GiovannaCarluccio Maria Annunziata - Rosmarinic acid (RA), a polyphenolic ester abundant in Melissa officinalis, exhibits potent antioxidant properties. While the traditional use of M. officinalis for detoxification is well documented, the molecular mechanisms by which RA protects against drug-induced acute kidney injury (AKI), particularly whether it actively induces endogenous cytoprotective pathways, remain incompletely defined. - Source: PubMed
Publication date: 2026/05/11
Salako Olatunji NozeemDesimone MartinEze Vincent ChukwuemekaAkingbade Modupe Adebanji - Acute liver injury (ALI) is a severe public health problem closely associated with oxidative stress, inflammation, and hepatocyte injury, leading to high mortality. Fucoxanthin (Fx), a marine carotenoid found in brown seaweeds, has various beneficial effects against multiple diseases. However, the potential role of Fx on ALI remains unclear. This study aims to explore the pharmacological potential of Fx in lipopolysaccharide (LPS)/D-galactosamine (D-Gal)-induced ALI. The therapeutic effect of Fx on ALI was primarily evaluated using a mouse model induced by LPS/D-Gal, focusing on pathological changes, oxidative stress, inflammation, and pyroptosis. Additionally, the effects of Fx on cell pyroptosis and its molecular mechanisms were explored in an in vitro pyroptosis model established by inducing macrophages with LPS/Nigericin. Fx significantly alleviated the LPS/D-Gal-induced histopathological progression and hepatocyte apoptosis, reducing plasma levels of ALT, AST, and LDH. It also obviously decreased hepatic MDA levels while increasing antioxidant enzyme activities and GSH concentration compared to LPS/D-Gal-treated mice. These antioxidant effects were linked to the upregulation of hepatic Nrf-2, HO-1, and GCLC expression. Furthermore, Fx treatment alleviated macrophage accumulation and downregulated the expression of pro-inflammatory factors in the liver. Importantly, Fx administration suppressed NLRP3 inflammasome-dependent canonical pyroptosis both in LPS/D-Gal-treated mice and LPS/Nigericin-stimulated macrophages, potentially mediated by the suppression of MAPKs and NF-κB pathways. These findings suggest that Fx could be an effective strategy to prevent ALI, particularly in cases associated with NLRP3 inflammasome-mediated pyroptosis. - Source: PubMed
Publication date: 2026/05/11
Zheng YigangYing HangluShi JiayiYang HuiYang LongheWang NingZhao YufenMa HuabinLi Long - Moringa oleifera leaf extract (MOLE) is a carbohydrate-rich plant extract that may support intestinal homeostasis. The colon, as the principal site of the fermentation of indigestible carbohydrate, is susceptible to barrier disruption. However, the effects of MOLE on colonic health in piglets and the underlying mechanisms remain unclear. This study systematically characterized the composition and in vitro antioxidant activity of MOLE, evaluated its effects on colonic health in piglets, and explored the potential mechanisms using the fermentation of colonic digesta and an intestinal porcine epithelial cell line J2 (IPEC-J2) oxidative stress model. - Source: PubMed
Publication date: 2026/05/10
Liu ZiwenDing LirenDaniel SindayeHou MeixinWu JianminMiao JingnaSui ZhiyuanDeng WenzhuoHang SuqinXiang Xiaoe - Understanding the molecular mechanisms underlying normal hair follicle development is important for improving wool traits in sheep and skin traits in mammals. This study aims to investigate the genetic determinants influencing hair follicle (HF) development by conducting an integrated analysis of transcriptomic and proteomic datasets from skin tissues of adult Dorper ewes at different groups of shedding and non-shedding. We employed DIA quantitative proteomics technology to identify 2176 differentially abundant proteins (DAPs) across three stages in both the shedding and non-shedding groups. Six DAPs were validated using parallel reaction monitoring (PRM) to confirm the reliability of the discovery proteomics data. Functional enrichment analysis revealed that a series of biological processes and signaling pathways associated with HF development, such as glutathione metabolism, ferroptosis, Wnt, JAK-STAT, and PI3K-Akt pathways, were strongly enriched by these DAPs. Association analysis and protein interaction network analysis further indicated that ACTG1, ANPEP, CTNNB1, GCLC, GCLM, CSNK2A2, COL1A1, GLUL, VTN, CDK6, IKBKG, STAM2, ITGA3, and members of the keratin (KRT) family may be key factors contributing to the developmental differences in the hair follicle cycle. Our study contributes to the understanding of the genetic and proteomic mechanisms underlying the normal growth and development of hair follicles and mammalian skin-related traits. SIGNIFICANCE: The morphology of secondary hair follicles in Dorper sheep plays a crucial role in determining the hair follicle cycle. The hair follicle cycle, as well as hair follicle growth and morphogenesis, are coordinated and complex processes. This study constructed a protein regulatory network within the signaling pathways associated with the cyclic growth and development of Dorper sheep hair follicles, and investigated key genes whose expression levels were positively correlated between mRNA and protein. Understanding the genetic mechanisms underlying the hair-shedding ability of Dorper sheep is crucial for enhancing the economic value of meat sheep and advancing the breeding of automatically hair-shedding sheep. - Source: PubMed
Publication date: 2026/05/08
Wang SiyuZhang NingyueWang ChunguangJing BinYang FanLi Xinhai