Ask about this productRelated genes to: Chodl antibody
- Gene:
- CHODL NIH gene
- Name:
- chondrolectin
- Previous symbol:
- C21orf68
- Synonyms:
- FLJ12627, PRED12, MT75
- Chromosome:
- 21q21.1
- Locus Type:
- gene with protein product
- Date approved:
- 2001-12-14
- Date modifiied:
- 2016-10-05
Related products to: Chodl antibody
Related articles to: Chodl antibody
- Population stratification based on gut microbiota composition has revealed several enterotypes in humans and animals, providing valuable tools for studying the gut microbiota landscape, which is crucial for animal health and production. However, knowledge about rumen enterotype identification in sheep, its influencing factors, and its association with growth performance and host genetics remains limited. Here, we investigated host genetic effects and phenotypic landscapes of rumen bacterial enterotypes in a large sheep population. Ruminal contents from 1150 healthy sheep were analyzed using 16S rRNA gene sequencing and genus-level clustering, complemented by extensive phenotypic data covering 47 traits spanning growth, feed efficiency, meat yield, and ruminal fermentation, along with whole-genome resequencing data. We identified two distinct enterotypes: Enterotype 1 (E1), a mixture of multiple genera, and Enterotype 2 (E2), dominated by . E2 sheep exhibit superior growth and meat production performance, but lower feed efficiency and increased fat deposition. Two-part beta-regression models and co-occurrence network analyses revealed the extensive impact of enterotypes on microbial community structure, with E1 displaying a higher frequency of unique bacterial interactions. The estimated heritability of the enterotype was 0.47, and a GWAS identified five key genetic markers associated with rumen enterotype, localized to two candidate genes: and . These markers significantly influence 58 ruminal bacterial genera, including key taxa and driving genus. Overall, our data provide new insights into sheep rumen-enterotype characteristics, contributing to a better understanding of microbial interactions that are crucial for improving ruminant growth performance. - Source: PubMed
Publication date: 2025/09/17
Zhang YukunLi FadiZhang XiaoxueZhang DeyinWang Weimin - Neuropsychiatric disorders have a strong genetic component and are linked to developmental risk factors, yet it is unclear why symptoms appear only later in life and which neuronal types contribute to brain dysfunction. We addressed these questions using a robust mouse model of a neuropsychiatric syndrome-the 15q13.3 microdeletion. Single-nucleus transcriptomics revealed the largest gene expression alterations in the somatostatin (Sst) Sst_Chodl subtype, the long-range γ-aminobutyric acid (GABAergic) projecting neurons. Despite the developmental onset of perturbations, impairments in Sst_Chodl neurons manifested only at late maturation. Calcium imaging and patch-clamp recordings unraveled impaired responsivity overall in deep-layer Sst neurons, with only the Sst_Chodl subtype exhibiting increased activity. Patch-seq analysis connected molecular changes to cellular dysfunction of Sst_Chodl neurons. Finally, microdeletion mice displayed sleep disturbances associated with impaired activity of deep-layer Sst neurons, which were rescued by chemogenetic inhibition of Sst_Chodl neurons. Our findings spotlight GABAergic projection neurons as potential vulnerable targets in neuropsychiatric disorders. - Source: PubMed
Publication date: 2025/09/24
Asenjo-Martinez AndreaDragicevic KatarinaHou Wen-HsienOzsvar AttilaWinther Hansen NikolajPfisterer UlrichRydbirk RasmusDemharter SamuelMøller Bente EmmaGasthaus JaninaKorshunova IrinaPerrier Jean-FrançoisCapogna MarcoVasistha Navneet AKhodosevich Konstantin - Skeletal muscle satellite cells (SCs) reside between the myofiber sarcolemma and basal lamina, where extracellular matrix (ECM) interactions are essential for their maintenance and regenerative function. Here, we identify chondrolectin (CHODL), a type I transmembrane protein with a C-type lectin domain, as a critical regulator of SC biology. Single-cell RNA-seq analysis reveals that is highly enriched in quiescent SCs but downregulated in proliferating myoblasts. Using conditional knockout models, we show that deletion of in embryonic myoblasts ( ) or adult SCs ( ) does not affect muscle development but markedly impairs regeneration in both young and aged mice. -deficient SCs exhibit reduced self-renewal, diminish proliferation, and impair differentiation, leading to defective myofiber repair. In silico network perturbation further predicts disruption of ECM-ligand interactions and Notch signaling, consistent with our observation that a significant fraction of SCs in mice localize outside the basal lamina and undergo precocious activation. Together, these findings establish CHODL as a key determinant of SC niche localization and regenerative function, uncovering a previously unrecognized mechanism linking ECM interactions to muscle stem cell maintenance and repair. - Source: PubMed
Publication date: 2025/09/13
Gu LijieKim Kun HoChen XiyueOprescu Stephanie NLi YufenRen JunxiaoKuang ShihuanYue Feng - The intricate relationship between gut microbiota and various physiological functions in animals has emerged as a focal point in understanding host adaptability. Unlike the native birds of the Qinghai-Tibet Plateau (QTP), the tree sparrow () is believed to have colonized the plateau within the last few thousand years. Given the vast expanse and harsh conditions of the plateau, the role of gut microbiota in facilitating the tree sparrow's adaptation to this high-altitude habitat remains largely unexplored and holds significant scientific interest. Therefore, we employed a multidisciplinary approach combining amplicon sequencing, transcriptome analysis, and fecal microbiota transplantation (FMT) to investigate the functional role of gut microbiota in high-altitude tree sparrows across different seasons. Results indicate that the gut microbiota of tree sparrows exhibits seasonal and altitude-dependent changes, with an increase in in winter, which may promote heat production to cope with the cold. FMT experiments confirmed that "high-altitude gut microbiota" enhances the expression of heat-related proteins (avUCP) and upregulates heat-related genes and . These findings suggest an adaptive strategy whereby tree sparrows utilize their gut microbiota to modulate energy metabolism, ultimately conserving energy in the resource-limited high-altitude environment. - Source: PubMed
Publication date: 2025/07/31
Bo TingbeiSong GangZhang MengruXu XiaomingDuan JundongShe HuishangFang YunLi WentingWen JingLiu JingsongWang DehuaLei Fumin - We present an enhancer-AAV toolbox for accessing and perturbing striatal cell types and circuits. Best-in-class vectors were curated for accessing major striatal neuron populations including medium spiny neurons (MSNs), direct- and indirect-pathway MSNs, Sst-Chodl, Pvalb-Pthlh, and cholinergic interneurons. Specificity was evaluated by multiple modes of molecular validation, by three different routes of virus delivery, and with diverse transgene cargos. Importantly, we provide detailed information necessary to achieve reliable cell-type-specific labeling under different experimental contexts. We demonstrate direct pathway circuit-selective optogenetic perturbation of behavior and multiplex labeling of striatal interneuron types for targeted analysis of cellular features. Lastly, we show conserved in vivo activity for exemplary MSN enhancers in rats and macaques. This collection of striatal enhancer AAVs offers greater versatility compared to available transgenic lines and can readily be applied for cell type and circuit studies in diverse mammalian species beyond the mouse model. - Source: PubMed
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