Ask about this productRelated genes to: Katna1 antibody
- Gene:
- KATNA1 NIH gene
- Name:
- katanin catalytic subunit A1
- Previous symbol:
- -
- Synonyms:
- -
- Chromosome:
- 6q25.1
- Locus Type:
- gene with protein product
- Date approved:
- 1999-08-25
- Date modifiied:
- 2016-10-05
Related products to: Katna1 antibody
Related articles to: Katna1 antibody
- Cytoskeletal remodeling, including microtubule severing, is essential for neurite growth and structural plasticity. However, the regulatory mechanisms that control the microtubule-severing activity of katanin A1 (KATNA1) remain incompletely understood. Here, we identify a novel interaction between eukaryotic translation elongation factor 1 beta (EEF1B2) and KATNA1 and show that this protein pair regulates microtubule dynamics and neurite outgrowth. Proteomic screening and biochemical validation (GST pull-down and co-immunoprecipitation) demonstrated that EEF1B2 interacted with KATNA1, with strongest binding mapped to the KATNA1 AAA + ATPase domain. In COS7 cells, EEF1B2 potentiated KATNA1-dependent microtubule severing, whereas EEF1B2 knockdown attenuated KATNA1-driven microtubule loss. In primary cortical neurons, EEF1B2 enhanced KATNA1-induced neurite outgrowth and branching, and these effects were KATNA1-dependent. Together, these findings identify EEF1B2 as a modulator of KATNA1 activity and provide new insight into the molecular mechanisms governing microtubule remodeling and neurite growth. - Source: PubMed
Publication date: 2026/04/17
Wu ZengzhiYan JiajieDeng TingtingDong JiaoMeng ZhichaoZhu JiehaoChen MuZhang GuoweiTan WeiTan Minghui - Understanding how genomic variants contribute to lung cancer (LC) risk is key to better understanding the molecular mechanisms underlying that risk. While genome-wide association studies (GWAS) have identified numerous LC risk loci, most single nucleotide polymorphisms (SNPs) reside in non-coding regions, making the interpretation of their function challenging. We accounted for lung-specific chromatin interactions and allele-specific gene expression levels in lung tissue to identify novel interactions between LC GWAS SNPs and distal genes. Pathway enrichment analysis implicated eight target genes (CYP2A6, ADCY2, CHRNA3, CHRNA5, LATS1, RAD52, RIF1, TP53BP1) in functional networks involving caffeine metabolism, DNA ionizing radiation (IR)-double strand breaks and cellular response, and nicotine effect on dopaminergic neurons. Novel findings include a role for rs2853677 in ADCY2 dysregulation (previous attribution to TERT) and rs9322193 in targeting tumour suppressor gene LATS1 (previous attribution to RPS18P9/KATNA1). By linking germline variants to more biologically relevant gene targets and somatic processes, our results align more closely with established epidemiological and environmental risk factors for lung cancer, including a potential genetic explanation for the environmental interaction of caffeine and smoking in LC risk. This highlights the value of integrating 3D genome architecture and tissue-specific expression to refine our understanding of cancer susceptibility. - Source: PubMed
Publication date: 2025/11/13
Khoo AldricPudjihartono MichaelO'Sullivan Justin MSchierding William - Katanins are microtubule severing enzymes that play roles in diverse cell functions including meiotic and mitotic spindle formation. To address the role of Katanin p60 isozymes in mammalian oocytes, we have used the ZP3-CreLox approach to specifically delete Katanin A1 (KATNA1) and Katanin A-like 1 (KATNAL1) from the start of oocyte growth. Here, we show that KATNAL1 is not required for normal fertility, but deletion of KATNA1 causes a 50% decrease in fertility. Further investigation in Katna1-/- oocytes revealed no effect on MI spindle morphology but a modest effect on the morphology of MII spindles. This was accompanied by a decreased rate of fertilization, but Katna1-/+ heterozygous embryos that reached the 2-cell stage developed at normal rates to the blastocyst stage. Parthenogenetic activation of Katna1-/- oocytes to generate diploid homozygous embryos revealed a reduced rate of blastocyst formation. Further, the Katna1-/- parthenogenetic blastocysts had a reduced diameter, decreased cell number, and increased nuclear size. Taken together, our data indicate KATNA1, but not KATNAL1, plays a role in MII spindle function and mitotic cell divisions of the preimplantation embryo. The ability of the paternal allele to rescue preimplantation development suggests the origin of the decrease in the fertility of conditional Katna1-/- mice lies in abnormalities arising in the egg to embryo transition prior to embryonic genome activation. - Source: PubMed
Yuen Wai ShanZhang Qing-HuaDunstan MoniqueAdhikari DeepakO'Connor Anne EDunleavy Jessica E MO'Bryan Moira KCarroll John - The polymerization and severing of microtubules are fundamental to the growth and branching of neurites in hippocampal neurons. The catalytic ATPase-containing A-subunit of katanin p60 (p60, KATNA1) promotes growth and development of hippocampal neurites by severing microtubules, while collapsing response mediator protein 3 (CRMP3) assembles microtubules to regulate neurite outgrowth. However, whether microtubule severing and assembling proteins would work together to regulate neurite outgrowth, especially for KATNA1 and CRMP3 remains to be elucidated. In this study, we revealed the interaction between KATNA1 and CRMP3 through GST-pulldown and co-immunoprecipitation assays and identified the binding domains between KATNA1 and CRMP3 as the MIT of KATNA1 (residues 1-77) and the D region of CRMP3 (residues 64-413). Furthermore, we demonstrated that CRMP3 enhances the microtubule-severing efficiency of KATNA1. In cultured hippocampal neurons, overexpression of KATNA1 and CRMP3 increased neurite length and branch number, and co-expression of both proteins further enhanced the promoting effect. Moreover, genetic knockout of KATNA1 or/and CRMP3 significantly inhibited neurite outgrowth. Overall, our data suggest that the CRMP3 interaction enhances the severing activity of KATNA1, thereby promoting hippocampal neurite outgrowth. - Source: PubMed
Publication date: 2025/01/30
Xie ZhiyaoSong WeiMeng ZhichaoMa AoZhu JiehaoLiang YaozhongLin HongshengLei ChangbinTan Minghui - Nor1/NR4A3 is a member of the NR4A subfamily of nuclear receptors that play essential roles in regulating gene expression related to development, cell homeostasis and neurological functions. However, Nor1 is still considered an orphan receptor, as its natural ligand remains unclear for mediating transcriptional activation. Yet other activation signals may modulate Nor1 activity, although their precise role in the development and maintenance of the nervous system remains elusive. - Source: PubMed
Publication date: 2024/07/13
Gagnon JonathanCaron VéroniqueTremblay André