Ask about this productRelated genes to: Plk3 antibody
- Gene:
- PLK3 NIH gene
- Name:
- polo like kinase 3
- Previous symbol:
- CNK
- Synonyms:
- FNK, PRK
- Chromosome:
- 1p34.1
- Locus Type:
- gene with protein product
- Date approved:
- 1997-06-12
- Date modifiied:
- 2016-01-22
Related products to: Plk3 antibody
Related articles to: Plk3 antibody
- Vitamin D is one of the most popular supplements worldwide, yet its appropriate dosage and full impact on health of humans and animals are still debatable. In this study, 30 pigs were divided into three groups, differing in the amount of vitamin D in the diet (Group A - no supplementation, group B-5000 IU/Kg of vitamin D, and group C 10000 IU/Kg). After 3 months of fattening, animals were slaughtered, and samples of jejunum (the longest part of the small intestine in pigs) and colon were collected for transcriptome analysis. Comparison of the transcriptomes between jejunum and colon identified 3872 Differentially Expressed Genes (DEGs). In contrast, transcriptomic changes under the influence of vitamin D were subtle in both parts of the intestine. RNA-seq results showed that vitamin D supplementation with 5000 IU/Kg enhanced the expression of 7 genes in the jejunum and one gene (MEP1B) in the colon (FDR < 0.05, base mean > 10, and log2fold change>0.6), while supplementation with 10,000 IU/Kg increased the expression of one gene (OASL) in the jejunum. No DEGs with FDR < 0.05 were identified after supplementation with 10,000 IU/kg of vitamin D in the colon, however qPCR analysis showed that genes connected to cell cycle control (PLK1, PLK3, KIF4A, KIFC1, AURKB) are upregulated in this group. Gene Set enrichment analysis of the whole RNA-seq dataset revealed that among the most affected by vitamin D processes are that connected to immunity, especially antiviral response in the jejunum, and that connected to cell cycle control in the colon. Despite the use of very high dietary vitamin D doses, no evidence of overt intestinal toxicity was observed at the transcriptomic level. Nevertheless, the activation of molecular pathways involved in calcium handling and cell cycle regulation suggests that prolonged exposure to supraphysiological vitamin D levels may trigger adaptive responses whose long-term consequences remain unknown. - Source: PubMed
Publication date: 2026/04/19
Oczkowicz MariaWierzbicka AlicjaŚwiątkiewicz MałgorzataSzmatoła TomaszSteg AnnaSmołucha Grzegorz - Polo-like Kinase 1 (PLK1) plays essential roles in the S, G2, and M phases of the cell cycle, and its overexpression is frequently observed in multiple cancers, including breast cancer, where it contributes to genomic instability and dysregulated apoptosis. Unlike conventional ATP-competitive inhibitors that target the kinase domain, selective inhibition of PLK1's polo-box domain (PBD) offers a promising strategy to disrupt protein-ligand interactions critical for mitotic progression, thereby triggering apoptosis in cancer cells. However, the high structural similarity between PLK1 and its homologs (PLK2 and PLK3), which are vital for neurological function and the stress response, respectively, necessitates exceptional selectivity to avoid off-target effects. To address this challenge, the protocol entails a bilingual (American Sign Language and English) computational workflow that integrates virtual screening, structural clustering, protein-ligand docking, binding affinity prediction, ADMET-S profiling, and quantum mechanical (QM) stability analysis. Starting from the SuperNatural 3.0 natural product database, compounds were filtered using breast cancer relevance and drug-likeness criteria, clustered to ensure chemical diversity, and evaluated their interactions with PLK1-, PLK2-, and PLK3-PBD structures. While virtual docking and in silico ADMET-S assessments cannot definitively confirm selectivity or mechanism of action, this study generates testable hypotheses and prioritizes a focused set of natural-product-derived candidates for future molecular dynamics simulations, biochemical validation, or experimental screening. - Source: PubMed
Publication date: 2026/04/03
Mbogo Blessed PFernsebner Samantha RLawal Monsurat MLundberg Daniel JKucukkal Tugba G - Older maternal age at first vaginal delivery, defined in some studies as ≥30 years old, confers a significant increased risk of pelvic organ prolapse. Age-related impairment of postpartum recovery of levator ani muscles may help explain this association; yet, little is known about the biomolecular changes that occur in the levator ani after vaginal delivery, or with aging. - Source: PubMed
Publication date: 2026/04/15
Swenson Carolyn WPouladi NimaZabriskie Hannah ABourrant Paul-EmileLi JianrongWilson Liam SDrummond Micah JLussier Yves A - Polo-like kinase 3 (PLK3) plays major roles in cell cycle regulation, DNA repair, and cellular responses to hypoxia. Our prior studies demonstrated that PLK3 negatively regulates the hypoxic response by directly phosphorylating and destabilizing HIF-1α and by destabilizing the E3 ubiquitin ligase SIAH2. We also find that PLK3 stabilizes PTEN by direct phosphorylation. Plk3 knockout mice exhibit increased spontaneous tumorigenesis in multiple organs, particularly the lung, at an advanced age. Tumors from these mice tend to be highly vascularized, consistent with the function of PLK3 in the hypoxic response. However, another study only observed increased tumorigenesis in female Plk3 knockout mice. The present study further explored the role of PLK3 in lung tumorigenesis. We find that PLK3 can phosphorylate SIAH2 in vitro, confirming our hypothesis that PLK3 regulates SIAH2 by direct phosphorylation. We detected a negative correlation between the levels of PLK3 and SIAH2 and a positive correlation between HIF-1α and SIAH2 in both human lung adenocarcinoma and squamous cell carcinoma. We observed an increase in lung tumorigenesis in Plk3 knockout mice in the A/J strain background. Our RNA-Seq analysis revealed significantly increased expression of genes involved in oncogenic pathways and the immune response in lung tumors from Plk3 knockout mice. Finally, we find that induced systemic SIAH2 expression promotes CD8 T cell infiltration into subcutaneous tumors in a syngeneic mouse model. Our work further supports the tumor suppressive role of PLK3 in lung cancer and discovered a novel involvement of PLK3 in the regulation of the immune microenvironment of lung tumors. - Source: PubMed
Li CenSamad ArkoOstrowski CaseyAlam S M ShafiqulYin ChanghongAyyamperumal SelvarajChen JinjinZhong MinghaoHuang WeihuaWang YinshengIslam Humayun KPhillips John LLee Marietta Y W TXu Dazhong - Small molecule anti-cancer drugs can cause adverse effects; most commonly low blood cell counts. Protein kinase D2 (PKD2) is highly expressed in neutrophils, and this study investigated the role of PKD2 inhibition on the survival of human neutrophils using small molecule compounds. - Source: PubMed
Uchiyama Noriko