Ask about this productRelated genes to: HYAL1 antibody
- Gene:
- HYAL1 NIH gene
- Name:
- hyaluronidase 1
- Previous symbol:
- -
- Synonyms:
- LUCA1, HYAL-1
- Chromosome:
- 3p21.31
- Locus Type:
- gene with protein product
- Date approved:
- 1997-10-09
- Date modifiied:
- 2018-08-02
Related products to: HYAL1 antibody
Related articles to: HYAL1 antibody
- Hyaluronan (HA), a polysaccharide present in tissues throughout the body, exhibits various physiological functions depending on its molecular weight. Although high-molecular-weight HA (HM-HA) is produced in large amounts in the epidermal basal layer, low-molecular-weight HA (LM-HA) exists and acts as a moisturizing factor in the epidermal stratum corneum. However, the mechanism and physiological role of the HA metabolism in the epidermis remains unknown. The present study investigated the effects of various molecular weights of HA treatments and HA metabolism-related factor knockdown on the epidermal barrier function mediated by intercellular tight junctions (TJs) using human epidermal-derived HaCaT cells. The HA-metabolizing enzymes responsible for HA production and degradation in epidermal cells were also examined. Treatment with HM-HA (1400 kDa) decreased mRNA and protein expression of claudin-1 (CLDN1), which is an essential protein in the epidermal TJ component. In contrast, no change was observed in LM- HA (3 kDa). Knockdown of the HA receptor CD44 by siRNA suppressed the HM-HA-induced CLDN1 down-regulation. Among the HA-degrading enzymes, the knockdown of hyaluronidase (HYAL) 1, decreased HA-degrading activity and increased HA content in the lysates using a competition assay with an HA-binding protein. CLDN1 expression was decreased upon HYAL1 knockdown. These results suggest that HYAL1 is the main enzyme responsible for HA degradation in human epidermal keratinocytes. HYAL1 degrades HM-HA under acidic conditions, suggesting that HYAL1 expression is involved in TJ barrier regulation. The HYAL-mediated epidermal HA metabolism regulates TJ function. In the future, it is anticipated that HA metabolism-targeting novel therapeutics will be developed for skin diseases associated with TJ barrier dysfunction. - Source: PubMed
Publication date: 2026/03/23
Yoshino YutaGenkawa NatsukiHirose MaiNakamura SotaTanaka ToshihiroMiyamoto ChikaEndo SatoshiIkari Akira - To investigate the expression levels of enzymes and receptors of the hyaluronan (HA) pathway, including HA synthase (HAS)-2, hyaluronidase (Hyal)-1, Hyal-2, CD44 and receptor for HA-mediated motility (RHAMM) in the ocular microenvironment of patients with proliferative diabetic retinopathy (PDR) and the role of HA pathway in inflammation and angiogenesis that drive PDR initiation and progression. - Source: PubMed
Publication date: 2026/02/18
Abu El-Asrar Ahmed MNawaz Mohd IAhmad AjmalSiddiquei MairajAllegaert EefGikandi Priscilla WDe Hertogh GertOpdenakker Ghislain - : Fructans are fructose-based polysaccharides with diverse biological activities; however, their direct activity on skin cells remains unresolved. This study investigated the biological activity of fructan extracted from rakkyo () (RF) and examined its effects on extracellular matrix (ECM) metabolism, particularly collagen and hyaluronan synthesis, in human dermal fibroblasts. RF was prepared from fresh rakkyo bulbs by aqueous extraction, alkaline clarification, and membrane filtration. The average molecular weight and structural characteristics of RF were analyzed using size-exclusion chromatography and C NMR spectroscopy. Normal human dermal fibroblasts (NHDFs) were treated with RF by culturing cells in RF-supplemented medium (0.1-1.0 mg/mL). Cell viability and viable cell number were evaluated using the thiazolyl blue tetrazolium bromide and trypan blue exclusion assays, respectively. Expression of ECM-related genes was analyzed by qRT-PCR, and collagen and hyaluronan production were quantified by Sirius Red staining and ELISA. RF had an average molecular weight of approximately 11,500 Da and consisted of nearly equal proportions of inulin- and levan-type fructans. RF (≤1 mg/mL) increased the number of viable cells and markedly upregulated collagen, type I, alpha 1 () and hyaluronic acid synthase 2 () expression while downregulating expression. After 9 days of treatment, the cumulative production of type I collagen and hyaluronic acid increased by 3.8- and 1.3-fold, respectively, as compared with controls. Upregulation of lysyl oxidase () mRNA suggested enhanced collagen cross-linking, whereas showed only modest induction. Rakkyo-derived fructan directly stimulates collagen and hyaluronan synthesis in dermal fibroblasts, likely through regulation of ECM-related genes. These results suggest that rakkyo-derived fructan modulates ECM-related readouts in NHDFs under controlled in vitro conditions. Further validation in more complex skin models and in vivo studies is necessary. - Source: PubMed
Publication date: 2026/02/16
Tsukui KeiSano AikoKamioki KazumiDohgomori KiwamuKawaguchi Shin-IchiTokudome Yoshihiro - Idiopathic nephrotic syndrome (INS) is viewed as a podocyte-specific disease. Recent reports indicate endothelial involvement, but its significance is unclear. Here, we investigated the relationship between the glomerular expression of selected genes relevant to endothelial health and clinical markers of disease severity. - Source: PubMed
Publication date: 2025/12/26
Nelson-Taylor Sarah KTroost JonathanGiannini CourtneyBauer ColinSrivastava TarakZee JarcyBitzer MarkusBarisoni LauraDaehn LlseDougherty Julie ASmoyer William EKerlin Bryce AFetsko AudreyIslam ImtiazulWang XinSethna ChristineJohnson Richard Jde Lucas Collantes CarmenKaneko KazunariCara-Fuentes Gabriel - Hyaluronidases are widely distributed in nature being ubiquitous in snake species (svHyal). They catalyze the hydrolysis of β-1,4-glycosidic bonds in hyaluronic acid, a critical constituent of the extracellular matrix. This facilitates the spread of venom toxins into the bloodstream, exacerbating tissue damage and systemic toxicity─a rationale for their common designation as "spreading factors". While svHyals are not directly toxic, they substantially contribute to the morbidity and mortality associated with snakebite envenomation, the world's most lethal neglected tropical disease. Despite their important role in tissue penetration, the atomic-level reaction mechanism of these enzymes remains poorly understood. To bridge this knowledge gap, we studied the chemical mechanism of the Hyal-1 enzyme isolated from the Puff Adder viper (), likely the major contributor to snakebite mortality in sub-Saharan Africa. We evaluated two alternative mechanistic scenarios, based on different protonation states for the active site "assisting residue" (Asp110), and conducted umbrella sampling QM/MM MD simulations (PBE/DZVP-GTH-PBE: AMBER). Our findings indicate that the pathway starting from a neutral Asp110 yields an activation free energy barrier of 20.34 kcal·mol─nearly half that of the alternative pathway that considers an ionised Asp110. The deglycosylation step of the most favorable pathway yielded a free energy barrier of 13.94 kcal·mol. Our simulations also support an induced-fit mechanism for the svHyal/hyaluronic acid complex, with substrate distortion (chair → boat/skew-boat) favoring a conformation that closely mimics the transition state. This distortion, along with a prealignment of Glu112, lowers the activation free energy, enhancing the susceptibility of the glycosidic bond to nucleophilic attack. The results are likely transferable to all svHyal given their high degree of interspecific similarity (>90% sequence identity). This study highlights the importance of understanding mechanistics, including detailed stereoelectronic conformations and subsite-specific interactions, for the design of novel and effective inhibitors with broad clinical and biotechnological applications. - Source: PubMed
Publication date: 2026/01/26
Castro-Amorim JulianaRamos Maria JFernandes Pedro A