Ask about this productRelated genes to: MGC29891 antibody
- Gene:
- GABPB2 NIH gene
- Name:
- GA binding protein transcription factor subunit beta 2
- Previous symbol:
- -
- Synonyms:
- MGC29891
- Chromosome:
- 1q21.3
- Locus Type:
- gene with protein product
- Date approved:
- 2008-02-25
- Date modifiied:
- 2018-04-23
Related products to: MGC29891 antibody
Related articles to: MGC29891 antibody
- Meat quality is a critical factor influencing the sales of chicken. Most plant extracts have been shown to improve meat quality in poultry. This study selected 120 similar-weight (1.5 ± 0.2 kg) 135-day-old Jingyuan chickens, divided them into four groups, and fed them different doses (CON, 0.3 % FCE, 0.6 % FCE, and 0.9 % FCE) of fresh corn extract (FCE) until they were 180-day-old chickens. Fifteen chickens were randomly selected from each group for slaughter performance and meat quality assessment. Combining the transcriptome and metabolome sequencing data of the breast (CON and 0.6 % FCE), the weighted co-expression network analysis (WGCNA) method was used to identify hub genes and key metabolites significantly related to meat quality. The results showed that the 0.6 % FCE group was significantly better than the other groups in terms of slaughter performance and meat quality. Transcriptomic analysis identified differentially expressed genes (DEGs) significantly enriched in mineral absorption, amino sugar and nucleotide sugar metabolism, and phosphonate and phosphinate metabolism. Based on WGCNA, six key DEGs significantly associated with meat quality were selected. Metabolomics analysis identified differentially expressed metabolites (DEMs) significantly enriched in the pathways of secondary bile acid biosynthesis, autophagy, pantothenate and CoA biosynthesis, and beta-alanine metabolism Pearson correlation analysis further revealed correlations between the six key DEGs (YKT6, ENSGALG00010016848, GALK2, COMMD9, EIF2D, and GABPB2) and five key DEMs (1H-Indole-4-carboxaldehyde, Leucylproline, Trimethoprim, Ursodeoxycholic acid, and N.epsilon.-Acetyl-L-lysine). Furthermore, the expression levels and content of these genes and metabolites in the breast muscle of Jingyuan chickens were also assessed. - Source: PubMed
Publication date: 2025/10/30
Zhao WeiTian JinliYang LijuanXue LinChen SiyuMa RinminGu YalingWei DaweiZhang Juan - Alternative splicing (AS) contributes to transcript and protein diversity, affecting their structure and function. However, the specific transcriptional regulatory mechanisms underlying AS in the context of hepatic ischemia reperfusion (IR) injury in mice have not been extensively characterized. In this study, we investigated differentially alternatively spliced (DAS) genes and differentially expressed transcripts (DETs) in a mouse model of hepatic IR injury using the high throughput RNA sequencing (RNA-seq) analysis and replicate multivariate analysis of transcript splicing (rMATS) analysis. We further conducted Gene ontology (GO) term enrichment, the Kyoto Encyclopedia of Genes and Genomes (KEGG) database and the protein-protein interaction (PPI) network. A total of 898 DAS genes (p ≤ 0.05) were screened out in the hepatic IR group compared to the sham group, while functional enrichment analysis revealed that DETs and DAS genes were significantly associated with the ATP-dependent chromain, splicesome and metabolic pathways. The expression level of the DAS genes: Gabpb2, Smg1, Tnrc6c, Mettl17, Smpd4, Kcnt2, D16Ertd472e, Rab3gap2, Echdc2 and Ssx2ip were verified by RT-PCR and qRT-PCR. Our findings provide a comprehensive genome-wide view of AS events in hepatic IR injury in mice, enhancing our understanding of AS dynamics and the molecular mechanisms governing alternative pre-mRNA splicing. - Source: PubMed
Publication date: 2024/12/28
Hua YongliangLi XinglongYin BingLu ShounanQian BaolinZhou YongzhiLi ZhongyuMeng ZhanzhiMa Yong - Recent studies have shown that epithelial-mesenchymal transition (EMT) plays an important role in paraquat (PQ)-induced tissue fibrosis, which is the main cause of death in patients with PQ poisoning. However, no effective treatment for pulmonary interstitial fibrosis caused by PQ poisoning exists. It is of great significance for us to find new therapeutic targets through bioinformatics in PQ-induced EMT. We conducted transcriptome sequencing to determine the expression profiles of 1210 messenger RNAs (mRNAs), 558 long noncoding RNAs, 28 microRNAs (miRNAs), including 18 known-miRNAs, 10 novel-miRNAs and 154 circular RNAs in the PQ-exposed EMT group mice. Using gene ontology and Kyoto Encyclopaedia of Genes and Genomes analyses, we identified the pathways associated with signal transduction, cancers, endocrine systems and immune systems were involved in PQ-induced EMT. Furthermore, we constructed long noncoding RNA-miRNA-mRNA interrelated networks and found that upregulated genes included Il22ra2, Mdm4, Slc35e2 and Angptl4, and downregulated genes included RGS2, Gabpb2, Acvr1, Prkd3, Sp100, Tlr12, Syt15 and Camk2d. Thirteen new potential competitive endogenous RNA targets were also identified for further treatment of PQ-induced pulmonary tissue fibrosis. Through further study of the pathway and networks, we may identify new molecular targets in PQ-induced pulmonary EMT. - Source: PubMed
Ma ZhiyuWang NanaMeng TingtingZhang RuoyingHuang YangLi Tiegang - Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disease characterized by the progressive degeneration of motor neurons, leading to muscle atrophy, paralysis and even death. Immune disorder, redox imbalance, autophagy disorder, and iron homeostasis disorder have been shown to play critical roles in the pathogenesis of ALS. However, the exact pathogenic genes and the underlying mechanism of ALS remain unclear. The purpose of this study was to screen for pathogenic regulatory genes and prognostic markers in ALS using bioinformatics methods. We used Gene Ontology (GO) analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis, gene set enrichment analysis (GSEA), and expression regulation network analysis to investigate the function of differentially expressed genes in the nerve tissue, lymphoid tissue, and whole blood of patients with ALS. Our results showed that the up-regulated genes were mainly involved in immune regulation and inflammation, and the down-regulated genes were mainly involved in energy metabolism and redox processes. Eleven up-regulated transcription factors (CEBPB, CEBPD, STAT5A, STAT6, RUNX1, REL, SMAD3, GABPB2, FOXO1, PAX6, and FOXJ1) and one down-regulated transcription factor (NOG) in the nerve tissue of patients with ALS likely play important regulatory roles in the pathogenesis of ALS. Based on construction and evaluation of the ALS biomarker screening model, cluster analysis of the identified characteristic genes, univariate Cox proportional hazards regression analysis, and the random survival forest algorithm, we found that MAEA, TPST1, IFNGR2, and ALAS2 may be prognostic markers regarding the survival of ALS patients. High expression of MAEA, TPST1, and IFNGR2 and low expression of ALAS2 in ALS patients may be closely related to short survival of ALS patients. Taken together, our results indicate that immune disorders, inflammation, energy metabolism, and redox imbalance may be the important pathogenic factors of ALS. CEBPB, CEBPD, STAT5A, STAT6, RUNX1, REL, SMAD3, GABPB2, FOXO1, PAX6, FOXJ1, and NOG may be important regulatory factors linked to the pathogenesis of ALS. MAEA, TPST1, IFNGR2, and ALAS2 are potential important ALS prognostic markers. Our findings provide evidence on the pathogenesis of ALS, potential targets for the development of new drugs for ALS, and important markers for predicting ALS prognosis. - Source: PubMed
Publication date: 2022/02/01
Sun HualinLi MingJi YananZhu JianweiChen ZehaoZhang LileiDeng ChunyanCheng QiongWang WeiShen YuntianShen Dingding - Enhanced expression of TERT in gliomas is a result of two hotspot mutations, C228T and C250T, at the promoter region. GA-binding proteins selectively bind at these positions, respectively, causing an activation of the promoter and overexpression of TERT. GABP is a multimeric protein consisting of GABPA and GABPB with its isoforms GABPB1, GABPB1-L, GABPB1-S, GABPB2. In this study, we investigated the mRNA expression and association between TERT and GABPA/B isoforms in tumor samples of different glioma grades. The expression was determined by quantitative real-time PCR and the results were statistically analyzed. We present that TERT is mainly expressed in primary glioblastomas. All GA-binding proteins progress through the glioma grades and have the highest expression levels in secondary glioblastomas. In secondary glioblastomas after chemotherapy, GABPB1 and GABPB1-L are expressed on a lower level than without treatment. In high grades, TERT and GABPA, GAPB1, GABPB1-L, GABPB1-S are upregulated compared to low grades. Between primary and secondary glioblastomas with and without chemotherapy, TERT is elevated in the former while GABPB1 is increased in the secondary glioblastomas. GABPA and GABPB1, GABPB1-L and GABPB1-S positive correlate in primary glioblastomas. The present study confirms the upregulation of TERT in primary glioblastomas while all GABP proteins rise with the malignancy of the gliomas. Further investigations must be made to elucidate the relation between TERT and all GABP proteins as it may play a key role in the gliomagenesis. - Source: PubMed
Publication date: 2021/06/22
Papazacharias EfthymiosKuhl SaskiaRöhn GabrieleGörtz LukasGoldbrunner RolandTimmer Marco