Ask about this productRelated genes to: SCRT2 antibody
- Gene:
- SCRT2 NIH gene
- Name:
- scratch family transcriptional repressor 2
- Previous symbol:
- -
- Synonyms:
- ZNF898B
- Chromosome:
- 20p13
- Locus Type:
- gene with protein product
- Date approved:
- 2001-06-21
- Date modifiied:
- 2016-05-04
Related products to: SCRT2 antibody
Related articles to: SCRT2 antibody
- How transcriptional programs coordinate the transition from neural progenitors to lineage-committed neurons in the spinal cord remains poorly understood. While much is known about transcription factors acting in the proliferative and differentiated zones, the role of intermediate zone (IZ) factors during lineage specification is less clear. Here, we investigate the function of SCRATCH2 (SCRT2), expressed in the postmitotic cells of the IZ, during dorsal interneuron differentiation. Overexpression of SCRT2 in vivo reduced the number of ISLET1+ dorsal interneurons. Chromatin profiling revealed that SCRT2 primarily binds to intergenic, transcriptionally inactive regions near neurogenic genes. Among these, we identified a conserved regulatory element, ECR4, located between ISLET1 and PARP8. Functional assays showed that ECR4 drives neural transcription and is composed of two subregions: ECR4B, an enhancer activated by ISLET1 and POU4F1, and ECR4A, which contains SCRT2 binding motifs and mediates transcriptional repression. Mutation of the vCES-box, a predicted SCRT2-binding motif within ECR4A, abolished repression, confirming a repressive regulatory interaction. Together, these data support a model in which SCRT2 represses ISLET1 through ECR4 to modulate dI3 lineage specification. These findings identify a novel regulatory mechanism linking intermediate zone transcriptional repression to dorsal interneuron development in the spinal cord. - Source: PubMed
Publication date: 2025/08/14
Botezelli Vitória SKanno Tatiane YLiau Ee ShanGoes Carolina Pde La Cruz Anticona ShirleyAzambuja Ana PaulaSimoes-Costa MarcosYan C Y Irene - Isocitrate dehydrogenase-mutant gliomas are lethal brain cancers that impair quality of life in young adults. Although less aggressive than glioblastomas, IDH-mutant gliomas invariably progress to incurable disease with unpredictable recurrence. A better classification of patient risk of recurrence is needed. Here, we describe a multimodal analytical pipeline integrating imaging, transcriptomic, and proteomic profiles using machine learning to improve patient stratification with novel signatures of patient risk of recurrence based on gene expression, protein level, and imaging. Additionally, we describe the biological characteristics of IDH-mutant glioma subtypes categorized by positron emission tomography (PET) and histology, and we reinforce the integration of positron emission tomography (PET) metrics in the classification of IDH-mutant gliomas. We identify a gene signature (KRT19, RUNX3, and SCRT2) and a protein signature (ATXN10, EIF4H, ITGAV, and NCAM1) associated with an increased risk of early recurrence. Furthermore, we integrated these markers with imaging-derived features, obtaining a better stratification of IDH-mutant glioma patients in comparison to histomolecular classification alone. - Source: PubMed
Publication date: 2025/04/11
Chouleur TiffanieEtchegaray ChristèleVillain LauraLesur AntoineFerté ThomasRossi MarcoAndrique LaetitiaSimoncini CostanzaGiacobbi Anne-SophieGambaretti MatteoLopci EgestaFernades BethaniaDittmar GunnarBjerkvig RolfHejblum BorisThiébaut RodolpheSaut OlivierBello LorenzoBikfalvi Andreas - During the development of the mouse dentate gyrus (DG), granule neuronal progenitors (GNPs) arise from glial fibrillary acidic protein (GFAP)-expressing neural stem cells in the dentate notch. However, the transcriptional regulators that control their stepwise differentiation remain poorly defined. Since neurogenesis involves epithelial-to-mesenchymal transition (EMT)-like processes, we investigated the spatio-temporal expression profiles of the EMT transcription factors Zeb1, Scratch2 (Scrt2) and Nkx6-2 in relation to known GNP markers. Our results show that Zeb1 and Scrt2 exhibit sequential, but partially overlapping expression across embryonic and postnatal stages of GNP differentiation. Zeb1 is highly enriched in -GFP+/Sox2+ neural stem/progenitor pools and subsets of Tbr2+/Prox1+/NeuroD+ intermediate GNPs, whereas Scrt2 predominates in Tbr2+/Prox1+/NeuroD+ GNPs. Strikingly, the neuronal EMT regulator Nkx6-2 shows selective expression in postnatal Tbr2+/Prox1+ GNPs, but it is excluded from embryonic counterparts. This temporally coordinated yet distinct expression of Zeb1, Scrt2 and Nkx6-2 reveals discrete transcriptional programs orchestrating GNP differentiation and neurogenic progression at embryonic versus postnatal stages of DG neurogenesis. - Source: PubMed
Publication date: 2024/08/29
Ohyama KyojiShinohara Hiroshi MTakayama NatsumiOgawa RinaOmura ShoichiroHayashida MioTakahashi Tokiharu - ASCL1 is a transcription factor that directs neural progenitors towards lineage differentiation. Although many of the molecular mechanisms underlying its action have been described, several of its targets remain unidentified. We identified in the chick genome a putative enhancer (cE1) upstream of the transcription factor () locus with a predicted heterodimerization motif for ASCL1 and POU3F2. In this study, we investigated the role of ASCL1 and this enhancer in regulating the expression of the in the embryonic spinal cord. We confirmed that cE1 region interacted with the promoter. cE1 was sufficient to mediate ASCL1-driven expression in the neural tube through the heterodimerization sites. Moreover, expression was inhibited when we removed cE1 from the genome. These findings strongly indicate that ASCL1 regulates transcription in the neural tube through cE1. - Source: PubMed
Publication date: 2024/04/05
Goes Carolina PurcellBotezelli Vitória SamartinDe La Cruz Shirley MirnaCruz Mário CostaAzambuja Ana PaulaSimoes-Costa MarcosYan Chao Yun Irene - Chlorhexidine gluconate (CHG) is a topical antiseptic solution recommended for skin preparation before central venous catheter placement and maintenance in adults and children. Although CHG is not recommended for use in children aged <2 months owing to limited safety data, it is commonly used in neonatal intensive care units worldwide. We used zebrafish model to verify the effects of early-life exposure to CHG on the developing nervous system, highlighting its impact on oligodendrocyte development and myelination. - Source: PubMed
Publication date: 2022/07/19
Choi Eui KyungChoi Byung MinCho YujiKim Suhyun