Ask about this productRelated genes to: Dgat2 antibody
- Gene:
- DGAT2 NIH gene
- Name:
- diacylglycerol O-acyltransferase 2
- Previous symbol:
- -
- Synonyms:
- -
- Chromosome:
- 11q13.5
- Locus Type:
- gene with protein product
- Date approved:
- 2001-11-19
- Date modifiied:
- 2016-10-05
Related products to: Dgat2 antibody
Related articles to: Dgat2 antibody
- Meat quality characteristics such as juiciness, tenderness, and flavor are greatly enhanced by intramuscular fat (IMF). Improving intramuscular adipose tissue accumulation while maintaining stable lipid levels in fat depots has become a critical priority in the beef sector. Understanding the molecular mechanisms driving intramuscular fat deposition is critical because it can enable the utilization of genetic selection to produce high-quality beef. Emerging research suggests that miRNAs play essential roles in regulating IMF deposition, particularly through regulating preadipocyte dynamics such as proliferation and differentiation. Our study aimed to screen and explore the function of potential miRNA in bovine intramuscular preadipocytes and to delineate the underlying molecular mechanisms governing cell proliferation and differentiation. Through time series cluster analysis following by experimental validation by qPCR and Agarose gel PCR identified that bta-miR-380-3p was potential miRNA that highly related with intramuscular preadipocyes differentiation in beef cattle. Bta-miR-380-3p enhanced the proliferation of intramuscular pre-adipocytes by increasing the cell cycle progression and show dynamic time dependent regulation during proliferation and differentiation. It also boosted the formation of lipid droplets in intramuscular pre-adipocytes. The qPCR results revealed that bta-miR-380-3p mimic boosted the expression of PPARγ, FASN, FABP4, SREBP1, and DGAT2 differentiation marker genes. These results provide new insight on the regulatory processes of intramuscular adipogenesis and identify bta-miR-380-3p as a potential target for increasing beef quality through directional selection and molecular breeding. This finding provides a theoretical framework and molecular targets for increasing IMF content. - Source: PubMed
Publication date: 2026/04/26
Jilo Diba DedachaAbebe Belete KurazGuo JuntaoWang JianfangMa HaobinYang ZhimeiLiu HaibingCheng GongLi AnningZan Linsen - The increasing interest in seaweed as a potential feed and food source has prompted concerns regarding the presence of potentially toxic arsenic (As) species. Seaweed is known to contain the organic As species arsenosugars, but the toxicity of these compounds is not fully known due to a lack of scientific data. Nori (Porphyra spp.), a common red seaweed and potential feed and food candidate, contains arsenosugar-phosphate (As-Sug-PO) as a primary As species. In this study, As-Sug-PO was isolated from nori by solid-phase extraction (SPE) and subsequently evaluated for in vitro toxicity in primary salmon hepatocytes. The As-Sug-PO isolate, alongside As reference standards (arsenite As(III) and dimethylarsinic acid (DMA(V)), and arsenic-containing hydrocarbon (AsHC-360)), were assessed for cytotoxicity (mitochondrial activity) and for transcriptional effects on genes associated with lipid biosynthesis, inflammation and oxidative stress by reverse transcription-quantitative polymerase chain reaction (RT‑qPCR). Impacts on total As and As speciation were quantified in both cell pellets and culture medium using high-performance liquid chromatography (HPLC) coupled to inductively coupled plasma mass spectrometry (ICP-MS). High concentrations of As-Sug-PO caused cytotoxicity and mitochondrial damage, while lower levels influenced triglyceride biosynthesis, indicating impaired lipid deposition. As(III) was the most toxic As species, followed by As-Sug-PO and DMA(V) and unlike As(III), As-Sug-PO and DMA(V) did not alter total As levels and speciation profile in samples after 48 h of exposure. Follow-up in vivo studies with commercial standards are needed to confirm effects on lipid deposition and to support the establishment of regulatory limits for As-Sug-PO in feed and food. - Source: PubMed
Publication date: 2026/04/02
Ghazali MohammadSele VeronikaSahuquillo AngelesMorales-Rodríguez AlbaSilva Marta SofiaLópez-Sánchez Jose FerminDonald CareyBerntssen Marc H GSøfteland Liv - Triacylglycerol (TAG) serves as the primary storage lipid in plants, essential for seed germination and early seedling development. Acyl-CoA:diacylglycerol acyltransferase (DGAT) enzymes catalyze the last step in TAG synthesis by converting diacylglycerol (DAG) to TAG. Although functionally conserved across species, DGATs exhibit variations in substrate preference, among their other properties, highlighting their influence on fatty acid (FA) composition in TAG. In this study, we investigated the biochemical properties of Arabidopsis DGATs expressed in yeast with a primary emphasis on understanding the specificity and selectivity of these enzymes on different acyl acceptors and donors. One aim of the study was to investigate if the FA composition of TAG is due to DGAT selectivity in addition to being influenced by the distinct DAG pools (spatially separated de novo synthesized and phosphatidylcholine (PC)-derived DAG). Our findings showed that Arabidopsis (Arabidopsis thaliana) DGAT1 preferentially selects PC-derived DAGs. Further, we also report a new method for synthesizing sn-2,3-DAG that we used to study DGAT enantiomeric specificity. The results revealed that DGAT1 is specific towards the sn-1,2-DAG enantiomer whereas DGAT2 only utilizes sn-2,3-DAG, a substrate that is not directly involved in the Kennedy pathway. DGAT2 has so far not shown significant involvement in de novo TAG synthesis and thus our findings indicate a possible function for DGAT2 in Arabidopsis in TAG remodeling. - Source: PubMed
Publication date: 2026/04/21
Jaison Chinnu AnnBjörklund JoachimStymne StenDemski KamilHofvander PerLager Ida - Bone formation requires a substantial energy supply to sustain extracellular matrix production and mineralization, yet the temporal contribution of lipid metabolism during osteoblast maturation remains incompletely characterized. This study investigated the molecular and transcriptional remodeling of lipid metabolism. Intracellular lipid distribution was analyzed by confocal microscopy using Nile Red staining. Transcriptional modulation of lipid synthesis, storage, lipolysis, genes associated with mitochondrial fatty acid oxidation, and osteogenic markers were assessed by quantitative real-time PCR, and the biochemical composition was evaluated by Raman spectroscopy. Early stages of spheroid development showed higher expression of genes involved in lipid synthesis and storage (FASN, DGAT2, and PLIN2) together with intracellular lipid accumulation, whereas later stages displayed increased expression of lipolytic and β-oxidation markers (PNPLA2/ATGL, CPT1A, and HADHA), accompanied by the redistribution of lipid droplets. The Raman analysis revealed a time-dependent variation of lipid-associated CH/CH bands and modulation of protein-related Amide I-III signals, consistent with biochemical remodeling during maturation. Overall, the data indicate a coordinated transcriptional shift from lipid accumulation-associated pathways toward lipid mobilization during osteogenic progression in a 3D culture. This model provides a controlled experimental platform for investigating metabolic regulation during bone formation and for studying metabolic alterations associated with skeletal disorders. - Source: PubMed
Publication date: 2026/04/07
Rizzo Maria GiovannaMorganti DarioSciuto Emanuele LuigiSmeriglio AntonellaCannatà GiorgiaFazio BarbaraGuglielmino Salvatore P PTrombetta DomenicoFaggio CaterinaConoci Sabrina - Pulmonary tuberculosis (TB) remains a major global health challenge. The molecular and metabolic responses of monocyte-derived macrophages (MDMs), which are critical for host defense against (Mtb), are not fully characterized. A murine pulmonary TB model was established by intravenous injection of BALB/c mice with the attenuated Mtb strain H37Ra; controls received saline. After 8 weeks, lung MDMs were isolated for integrated transcriptomic and untargeted metabolomic profiling. Transcriptomic analysis identified 3970 differentially expressed genes (DEGs) in infected MDMs, including upregulated , , and and downregulated , , and . Metabolomic profiling revealed 113 differentially accumulated metabolites (DAMs). Taurocholic acid and linoleic acid were identified as potential diagnostic biomarkers, both achieving an area under the curve (AUC) of 1.0 in ROC analysis. Integrated omics analysis showed a positive correlation between linoleic acid levels and the expression of , , and , implicating lipid metabolic pathways in the host response to TB. This multi-omics study delineates key molecular and metabolic alterations in lung MDMs during TB infection. The identified metabolites, taurocholic acid and linoleic acid, show promise as biomarkers, while dysregulated linoleic acid metabolism represents a potential target for novel diagnostic and therapeutic strategies against TB. - Source: PubMed
Publication date: 2026/02/27
Sha YuxiaZhao XiaomanZhu HongyingLi YeShao MeilinDing ShenggangZhou Haoquan