Ask about this productRelated genes to: XPO5 antibody
- Gene:
- XPO5 NIH gene
- Name:
- exportin 5
- Previous symbol:
- -
- Synonyms:
- KIAA1291
- Chromosome:
- 6p21.1
- Locus Type:
- gene with protein product
- Date approved:
- 2003-03-10
- Date modifiied:
- 2014-11-19
Related products to: XPO5 antibody
Related articles to: XPO5 antibody
- : MicroRNAs (miRNAs) are key regulators of skeletal muscle adaptation; however, the extent to which exercise modulates the miRNA biogenesis pathway remains poorly understood. To investigate the impact of acute and chronic high-intensity exercise on components of miRNA biogenesis, and whether such changes are reflected in miRNA expression across stages of their biogenesis, we performed secondary analyses of muscle biopsy samples from two previously published studies. : Muscle biopsies were analyzed from the following protocols: nine men and eight women pre- and 3 h post- a bout of high-intensity interval cycling exercise (HIIE), and eleven men and eight women pre- and post- a 6-week period of high-intensity interval training (HIIT) or non-exercise control. mRNA expression of components of miRNA biogenesis including , , , and were assessed following HIIE using RT-qPCR and their protein abundance was measured following HIIT using Western blotting. Primary (, , ) and mature (, , ) miRNA expression were quantified following HIIT. : An acute bout of HIIE significantly decreased mRNA ( < 0.05) and resulted in a reduction in mRNA that approached significance ( < 0.10). Following 6 weeks of HIIT, no significant changes were detected in the protein abundance of Drosha, Exportin-5, Dicer, or Ago2. HIIT did not alter expression at either the primary or mature transcript level across all isoforms. : This study highlights the complexity of miRNA regulation in skeletal muscle and underscores the need for further research examining the temporal and mechanistic control of miRNA biogenesis in response to exercise. - Source: PubMed
Publication date: 2026/05/29
Wu ZeyuMenezes Eveline SSilva Natalia de M Lyra EArhen Benjamin BBeaupre Lucas P RSimpson Craig AMcGlory ChrisDe Felice Fernanda GGurd Brendon J - The small GTPase RAN plays a role in the biogenesis of mature miR-126, which is supplied by the bone marrow arterioles to leukemic stem cells (LSCs). MiR-126 supports the homeostasis of LSCs that initiate and maintain acute myeloid leukemia (AML). While therapeutic targeting of RAN has been difficult due to its structural features, through molecular dynamics simulations and docking studies, we have identified MAR-3.6.2 as a novel allosteric inhibitor that binds in a cryptic pocket in the C-terminal domain of RAN. We showed that MAR-3.6.2 disrupted RAN interaction with its guanine nucleotide exchange factor RCC1 and prevented the nuclear switch of RAN-GDP to RAN-GTP. This in turn led to RAN nuclear retention and reduced the RAN/XPO5-mediated export of pre-miR-126, thereby limiting mature miR-126 biogenesis in endothelial cells and their exogenous supply of mature miR-126 to LSCs. In a Mll/Flt3 AML murine model, MAR-3.6.2 reduced leukemia burden, prolonged survival, and decreased LSC frequency in secondary transplants. These findings highlight MAR-3.6.2 and future, potential derivatives as a promising small molecule-based approach to eradicate AML LSCs via inhibition of RAN/XPO5 trafficking and block of miR-126 biogenesis. - Source: PubMed
Publication date: 2026/04/14
Valerio MelissaWei WenyuanKang HyunjunMa NingBhattacharya SupriyoBaker GenevieveHu WeidongZhang LianjunHoang Dinh HoaFeng JiaZhang HongyuZhang BinPerry J Jefferson PVaidehi NagarajanNguyen Le Xuan TruongMarcucci Guido - While METTL1 is a well-established m7G writer protein, its protein-protein interaction network remains largely unexplored. To map the METTL1 interactome in HEK293T cells, we employed APEX2-mediated proximity labeling coupled with LC-MS/MS analysis. This approach allowed for the identification of 60 and 18 unique proteins significantly enriched in the METTL1 proximity proteome compared to enhanced green fluorescent protein (EGFP) and nuclear localization signal (NLS) controls, respectively. Among these proteins, we found exportin-5 (XPO5), a nuclear export factor critical for pre-miRNA transport. We validated the METTL1-XPO5 interaction by co-immunoprecipitation and western blot analysis. Strikingly, genetic ablation of METTL1 caused XPO5 to redistribute to the cytosol, which in turn accelerated pre-miRNA export and enhanced miRNA maturation. This function of METTL1 was independent of its canonical m7G methyltransferase activity. Mechanistically, we found that METTL1 facilitates ERK-mediated phosphorylation of XPO5, thereby promoting its nuclear retention. Accordingly, constitutive activation of ERK was sufficient to restore nuclear XPO5 localization in METTL1-deficient cells. In summary, our study uncovers a non-canonical role for METTL1 in regulating the subcellular distribution of XPO5 and pre-miRNA export, revealing a novel mechanism of miRNA maturation that extends METTL1's function beyond m7G methylation. - Source: PubMed
Cao ZhongwenChen XingyuanSun YuxiangWang Yinsheng - (1) This cross-sectional study aims to elucidate the association between the gene polymorphism (rs11544382) and colon cancer (CC). (2) Genotyping of (rs11544382) was performed in 120 individuals (60 CC patients and 60 controls) using real-time PCR (qPCR). Logistic regression and Chi-square (χ) tests were used for statistical analysis. (3) Evaluation of the gene polymorphism in CC and control groups revealed no statistically significant association between the mutant (GG) genotype and either the wild-type (AA) or heterozygous (AG) genotypes (χ = 2.07, = 0.151). The AG genotype was predominant in both patients (86.7%) and controls (91.7%). Smoking and alcohol consumption showed significant associations with CC ( < 0.05). Although the rs11544382 polymorphism was not associated with CC risk, this is a cross-sectional study. In light of these findings, larger and more comprehensive studies with increased sample size are required to clarify the relationship between the gene polymorphism (rs11544382) and CC. - Source: PubMed
Publication date: 2025/12/29
Agbektas TugbaGenc Husnu CagrıZontul CemileTas Ayca - Preterm infants are at risk for bilirubin-induced brain injury. Phototherapy is effective for lowering serum bilirubin but has potential adverse effects. The independent effects of hyperbilirubinemia and phototherapy on the hippocampal gene expression profile were determined using a preterm-equivalent Gunn rat model. - Source: PubMed
Publication date: 2025/12/22
Satrom Katherine MLock Eric FLund Troy CTran Phu VRao Raghavendra B